MHC Class I RT1A antibody | OX-18
Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 (MCA51GA) used for the detection of RT1A expressing cells in mouse brain by immunofluorescence on formalin fixed tissue cryosections.
Image caption:
MHCI molecules are expressed in the nucleus accumbens core in WT mice. Staining for MHCI with OX-18 antibody (upper-left), Neurogranin (upper-center) and the merged image (upper-right) are shown. Lower images represent negative controls without OX-18. Scale bar represents 50 μm.
From: Edamura M, Murakami G, Meng H, Itakura M, Shigemoto R, et al.. (2014)
Functional Deficiency of MHC Class I Enhances LTP and Abolishes LTD in the Nucleus Accumbens of Mice.
PLoS ONE 9(9): e107099.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rat MHC class I RT1A antibody, clone OX-18 (MCA51F) used to evaluate MHC class I expression on rat mesenchymal stem cells by flow cytometry.
Image caption:
Immunophenotyping and pro-inflammatory cytokine expression of untransduced and Ad-transduced MSCs. (A) Untransduced and Ad-transduced MSCs were stained for expression of the immunologically relevant markers MHC class I/II, CD80 and CD86 with specific antibodies and analysed by flow cytometry. (B) MSCs were transduced with Ad.GFP and after 24 h cells were collected and subjected to mRNA isolation and cDNA synthesis. RT-PCR analysis showing mRNA expression levels of IL-1β, IFN-γ and IL-6 from untransduced and Ad-transduced MSCs. Data shown are means ±SD of three independent experiments. 2E-ΔCT = 2−ΔCT→ number of copies of gene of interest relative to the number of copes of the internal control gene, β-actin.
From: Treacy O, Ryan AE, Heinzl T, O'Flynn L, Cregg M, et al. (2012)
Adenoviral Transduction of Mesenchymal Stem Cells: In Vitro Responses and In Vivo Immune Responses after Cell Transplantation.
PLoS ONE 7(8): e42662.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I RT1A antibody, clone OX-18 (MCA51R) used for the demonstration of MHC Class I expression and co-localization with PIT-1 protein by immunofluorescence on rat GH3 pituitary gland derived neoplastic cells.
Image caption:
PIT-1 protein was processed in the antigen presentation pathway in GH3 cells.
PIT-1 detected by the anti–PIT-1 antibody [15] demonstrated a co-localization with MHC class I molecules (Left).
PIT-1 detected by the patient serum [2] demonstrated a colocalization with MHC class I molecules (Right). Arrowheads indicate merged signals with each protein. Scale bar, 10 μm
From: Kanie K, et al.
Pathogenesis of Anti-PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells.
J Endocr Soc. 2019 Aug 26;3(11):1969-78.
doi: 10.1210/js.2019-00243.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I RT1A antibody, clone OX-18 (MCA51GA) used for the detection of MHC classI expressing cells in rat brain by immunohistochemistry of formalin fixed tissue cryosections.
Image caption:
Expression of major histocompatibility complex (MHC) class I and class II antigens in the right injected striatum. Photomicrographs were prepared from coronal sections through the striatum and processed for MHC class I (A–D) and MHC class II (F–I) immunocytochemistry in representative rats of the M PBS-Str hEPO (A and F), M hEPO-Str hEPO (B and G), Str PBS-Str hEPO (C and H) and Str hEPO-Str hEPO (D and I) groups 3 weeks after the second injections. The scale bar = 100 μm in I also for A–D and F–H. High magnification photomicrographs in the left bottom were prepared from the box areas in (A) and (H). (E) and (J) Arbitrary rating scores of MHC class I (E) and MHC class II (J) immunostaining in the right injected striatum of rats from the M PBS-Str hEPO, M hEPO-Str hEPO, Str PBS-Str hEPO and Str hEPO-Str hEPO groups. The bars represent the median values of rating scores, and the circles depict the individual values. Filled circles indicate the rats from which the photomicrographs were taken. A Kruskal-Wallis test followed by Mann-Whitney U tests was used to compare the rating scores between groups. * p<0.05 versus indicated groups.
From: Yang C, Yang W-H, Chen S-S, Ma B-F, Li B, et al. (2013)
Pre-immunization with an Intramuscular Injection of AAV9-Human Erythropoietin Vectors Reduces the Vector-Mediated Transduction following Re-Administration in Rat Brain.
PLoS ONE 8(5): e63876.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class I RT1A
- Product Type
- Monoclonal Antibody
- Clone
- OX-18
- Isotype
- IgG1
- Specificity
- MHC Class I RT1A
Antibody Quality and Performance Guarantee
| Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (Fukumoto et al. 1982). Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA51R, MCA51G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA51GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat spleen cell glycoproteins
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/100 |
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Immunohistology
- Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for MHC Class I RT1A antibody
-
Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
Eur J Immunol. 12 (3): 237-43. -
Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52: 631-40 -
Osawa, H. et al. (1985) Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens.
J Immunol. 134 (6): 3901-6. -
Chacon, M.A. & Boulanger, L.M. (2013) MHC class I protein is expressed by neurons and neural progenitors in mid-gestation mouse brain.
Mol Cell Neurosci. 52: 117-27. -
Fujikawa, L.S. et al. (1989) Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat.
Invest Ophthalmol Vis Sci. 30 (1): 66-73. -
Zhai, Y. and Knechtle, S. et al. (1998) Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro
Human Immunol. 59: 404-14 -
Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90. -
Edamura, M. et al. (2014) Functional deficiency of MHC class I enhances LTP and abolishes LTD in the nucleus accumbens of mice.
PLoS One. 9 (9): e107099.
View The Latest Product References
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Cunningham, T.L. et al. (2014) Correlations between blood-brain barrier disruption and neuroinflammation in an experimental model of penetrating ballistic-like brain injury.
J Neurotrauma. 31 (5): 505-14. -
Skwirba, M. et al. (2014) Expression of nestin after renal transplantation in the rat.
APMIS. 122 (10): 1020-31. -
Schu, S. et al. (2012) Immunogenicity of allogeneic mesenchymal stem cells.
J Cell Mol Med. 16 (9): 2094-103. -
Dixon-Salazar, T.J. et al. (2014) MHC class I limits hippocampal synapse density by inhibiting neuronal insulin receptor signaling.
J Neurosci. 34 (35): 11844-56. -
Yang, C. et al. (2013) Pre-immunization with an intramuscular injection of AAV9-human erythropoietin vectors reduces the vector-mediated transduction following re-administration in rat brain.
PLoS One. 8 (5): e63876. -
Elmer BM et al. (2013) MHCI requires MEF2 transcription factors to negatively regulate synapse density during development and in disease.
J Neurosci. 33 (34): 13791-804. -
Picarda E et al. (2014) MHC-derived allopeptide activates TCR-biased CD8+ Tregs and suppresses organ rejection.
J Clin Invest. 124 (6): 2497-512. -
Ma, R. et al. (2013) Structural integrity, ECM components and immunogenicity of decellularized laryngeal scaffold with preserved cartilage.
Biomaterials. 34 (7): 1790-8. -
Lu, X.C. et al. (2015) Dual Therapeutic Effects of C-10068, a Dextromethorphan Derivative, Against Post-Traumatic Nonconvulsive Seizures and Neuroinflammation in a Rat Model of Penetrating Ballistic-Like Brain Injury.
J Neurotrauma. 32 (20): 1621-32. -
Treacy, O. et al. (2012) Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
PLoS One. 7 (8): e42662. -
Inácio, R.F. et al. (2012) Interferon beta modulates major histocompatibility complex class I (MHC I) and CD3-zeta expression in PC12 cells.
Neurosci Lett. 513 (2): 223-8. -
Yang, Y.M. et al. (2013) Microglial TNF-α-dependent elevation of MHC class I expression on brain endothelium induced by amyloid-beta promotes T cell transendothelial migration.
Neurochem Res. 38 (11): 2295-304. -
Zhang, J.et al. (2017) Changes in Expressions of Major Histocompatibility Complex Class I, Paired-Immunoglobulin-Like Receptor B, and Cluster of Differentiation 3ζ in Motor Cortical Representations of the Brachial Plexus After Avulsion in Rats.
World Neurosurg. 106: 211-8. -
Otto, C. et al. (2012) Immunisation with an allogeneic peptide promotes the induction of antigen-specific MHC II(pos) CD4+ rat T cells demonstrating immunostimulatory properties.
Transpl Immunol. 26 (4): 220-9. -
Coiro, P. et al. (2015) Impaired synaptic development in a maternal immune activation mouse model of neurodevelopmental disorders.
Brain Behav Immun. pii: S0889-1591(15)00417-1. -
Bombeiro, A.L. et al. (2017) Importance of major histocompatibility complex of class I (MHC-I) expression for astroglial reactivity and stability of neural circuits in vitro.
Neurosci Lett. 647: 97-103. -
Iinuma, C. et al. (2015) Establishment of a vascular endothelial cell-reactive type II NKT cell clone from a rat model of autoimmune vasculitis.
Int Immunol. 27 (2): 105-14. -
Kanie, K. et al. (2019) Pathogenesis of Anti-PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells.
J Endocr Soc. 3 (11): 1969-78. -
Li, P. et al. (2020) Neuronal NLRC5 regulates MHC class I expression in Neuro-2a cells and also during hippocampal development.
J Neurochem. 152 (2): 182-94.
- RRID
- AB_322393
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