MHC Class I RT1A antibody | OX-18
Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (Fukumoto et al. 1982).
Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat spleen cell glycoproteins
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
- Pack Size: 0.1 mg, 1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
Applications of MHC Class I RT1A antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA1209||F||0.1 mg|
Product Specific References
References for MHC Class I RT1A antibody
Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
Eur J Immunol. 12 (3): 237-43.
Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52: 631-40
Osawa, H. et al. (1985) Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens.
J Immunol. 134 (6): 3901-6.
Chacon, M.A. & Boulanger, L.M. (2013) MHC class I protein is expressed by neurons and neural progenitors in mid-gestation mouse brain.
Mol Cell Neurosci. 52: 117-27.
Fujikawa, L.S. et al. (1989) Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat.
Invest Ophthalmol Vis Sci. 30 (1): 66-73.
Zhai, Y. and Knechtle, S. et al. (1998) Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro
Human Immunol. 59: 404-14
Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90.
Edamura, M. et al. (2014) Functional deficiency of MHC class I enhances LTP and abolishes LTD in the nucleus accumbens of mice.
PLoS One. 9 (9): e107099.
Cunningham, T.L. et al. (2014) Correlations between blood-brain barrier disruption and neuroinflammation in an experimental model of penetrating ballistic-like brain injury.
J Neurotrauma. 31 (5): 505-14.
Skwirba, M. et al. (2014) Expression of nestin after renal transplantation in the rat.
APMIS. 122 (10): 1020-31.
Schu, S. et al. (2012) Immunogenicity of allogeneic mesenchymal stem cells.
J Cell Mol Med. 16 (9): 2094-103.
Dixon-Salazar, T.J. et al. (2014) MHC class I limits hippocampal synapse density by inhibiting neuronal insulin receptor signaling.
J Neurosci. 34 (35): 11844-56.
Yang, C. et al. (2013) Pre-immunization with an intramuscular injection of AAV9-human erythropoietin vectors reduces the vector-mediated transduction following re-administration in rat brain.
PLoS One. 8 (5): e63876.
Elmer BM et al. (2013) MHCI requires MEF2 transcription factors to negatively regulate synapse density during development and in disease.
J Neurosci. 33 (34): 13791-804.
Picarda E et al. (2014) MHC-derived allopeptide activates TCR-biased CD8+ Tregs and suppresses organ rejection.
J Clin Invest. 124 (6): 2497-512.
Ma, R. et al. (2013) Structural integrity, ECM components and immunogenicity of decellularized laryngeal scaffold with preserved cartilage.
Biomaterials. 34 (7): 1790-8.
Lu, X.C. et al. (2015) Dual Therapeutic Effects of C-10068, a Dextromethorphan Derivative, Against Post-Traumatic Nonconvulsive Seizures and Neuroinflammation in a Rat Model of Penetrating Ballistic-Like Brain Injury.
J Neurotrauma. 32 (20): 1621-32.
Treacy, O. et al. (2012) Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
PLoS One. 7 (8): e42662.
Inácio, R.F. et al. (2012) Interferon beta modulates major histocompatibility complex class I (MHC I) and CD3-zeta expression in PC12 cells.
Neurosci Lett. 513 (2): 223-8.
Yang, Y.M. et al. (2013) Microglial TNF-α-dependent elevation of MHC class I expression on brain endothelium induced by amyloid-beta promotes T cell transendothelial migration.
Neurochem Res. 38 (11): 2295-304.
Zhang, J.et al. (2017) Changes in Expressions of Major Histocompatibility Complex Class I, Paired-Immunoglobulin-Like Receptor B, and Cluster of Differentiation 3ζ in Motor Cortical Representations of the Brachial Plexus After Avulsion in Rats.
World Neurosurg. 106: 211-8.
Otto, C. et al. (2012) Immunisation with an allogeneic peptide promotes the induction of antigen-specific MHC II(pos) CD4+ rat T cells demonstrating immunostimulatory properties.
Transpl Immunol. 26 (4): 220-9.
Coiro, P. et al. (2015) Impaired synaptic development in a maternal immune activation mouse model of neurodevelopmental disorders.
Brain Behav Immun. pii: S0889-1591(15)00417-1.
Bombeiro, A.L. et al. (2017) Importance of major histocompatibility complex of class I (MHC-I) expression for astroglial reactivity and stability of neural circuits in vitro.
Neurosci Lett. 647: 97-103.
Iinuma, C. et al. (2015) Establishment of a vascular endothelial cell-reactive type II NKT cell clone from a rat model of autoimmune vasculitis.
Int Immunol. 27 (2): 105-14.
Keitaro, K. et al. (2019) Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells
J Endoc Soc. 3 (11): 1969-78.
Kanie, K. et al. (2019) Pathogenesis of Anti-PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells.
J Endocr Soc. 3 (11): 1969-78.