MHC Class I RT1A antibody | OX-18

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Mouse anti Rat MHC Class I RT1A

Product Type
Monoclonal Antibody
Clone
OX-18
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA51GA C* E F IP 0.1 mg
MCA51R C* E F IP 0.25 mg
MCA51G C* E F IP 1 mg
Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized.

Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (Fukumoto et al. 1982).

Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat spleen cell glycoproteins
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Storage Information

Storage
Pack Size: 0.1 mg, 1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.25 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of MHC Class I RT1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for MHC Class I RT1A antibody

  1. Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.
    Eur J Immunol. 12 (3): 237-43.
  2. Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52: 631-40
  3. Osawa, H. et al. (1985) Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens.
    J Immunol. 134 (6): 3901-6.
  4. Chacon, M.A. & Boulanger, L.M. (2013) MHC class I protein is expressed by neurons and neural progenitors in mid-gestation mouse brain.
    Mol Cell Neurosci. 52: 117-27.
  5. Fujikawa, L.S. et al. (1989) Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat.
    Invest Ophthalmol Vis Sci. 30 (1): 66-73.
  6. Zhai, Y. and Knechtle, S. et al. (1998) Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro
    Human Immunol. 59: 404-14
  7. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
    Virology. 388: 78-90.
  8. Edamura, M. et al. (2014) Functional deficiency of MHC class I enhances LTP and abolishes LTD in the nucleus accumbens of mice.
    PLoS One. 9 (9): e107099.
  9. Cunningham, T.L. et al. (2014) Correlations between blood-brain barrier disruption and neuroinflammation in an experimental model of penetrating ballistic-like brain injury.
    J Neurotrauma. 31 (5): 505-14.
  10. Skwirba, M. et al. (2014) Expression of nestin after renal transplantation in the rat.
    APMIS. 122 (10): 1020-31.
  11. Schu, S. et al. (2012) Immunogenicity of allogeneic mesenchymal stem cells.
    J Cell Mol Med. 16 (9): 2094-103.
  12. Dixon-Salazar, T.J. et al. (2014) MHC class I limits hippocampal synapse density by inhibiting neuronal insulin receptor signaling.
    J Neurosci. 34 (35): 11844-56.
  13. Iinuma, C. et al. (2015) Establishment of a vascular endothelial cell-reactive type II NKT cell clone from a rat model of autoimmune vasculitis.
    Int Immunol. 27 (2): 105-14.
  14. Yang, C. et al. (2013) Pre-immunization with an intramuscular injection of AAV9-human erythropoietin vectors reduces the vector-mediated transduction following re-administration in rat brain.
    PLoS One. 8 (5): e63876.
  15. Elmer BM et al. (2013) MHCI requires MEF2 transcription factors to negatively regulate synapse density during development and in disease.
    J Neurosci. 33 (34): 13791-804.
  16. Picarda E et al. (2014) MHC-derived allopeptide activates TCR-biased CD8+ Tregs and suppresses organ rejection.
    J Clin Invest. 124 (6): 2497-512.
  17. Ma, R. et al. (2013) Structural integrity, ECM components and immunogenicity of decellularized laryngeal scaffold with preserved cartilage.
    Biomaterials. 34 (7): 1790-8.
  18. Lu, X.C. et al. (2015) Dual Therapeutic Effects of C-10068, a Dextromethorphan Derivative, Against Post-Traumatic Nonconvulsive Seizures and Neuroinflammation in a Rat Model of Penetrating Ballistic-Like Brain Injury.
    J Neurotrauma. 32 (20): 1621-32.
  19. Treacy, O. et al. (2012) Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
    PLoS One. 7 (8): e42662.
  20. Inácio, R.F. et al. (2012) Interferon beta modulates major histocompatibility complex class I (MHC I) and CD3-zeta expression in PC12 cells.
    Neurosci Lett. 513 (2): 223-8.
  21. Yang, Y.M. et al. (2013) Microglial TNF-α-dependent elevation of MHC class I expression on brain endothelium induced by amyloid-beta promotes T cell transendothelial migration.
    Neurochem Res. 38 (11): 2295-304.
  22. Zhang, J. et al. (2017) Changes in expressions of MHCI, PirB and CD3ζ in motor cortical representations of the brachial plexus after avulsion in rats.
    World Neurosurg. Jun 29. pii: S1878-8750(17)31028-8. [Epub ahead of print]
  23. Otto, C. et al. (2012) Immunisation with an allogeneic peptide promotes the induction of antigen-specific MHC II(pos) CD4+ rat T cells demonstrating immunostimulatory properties.
    Transpl Immunol. 26 (4): 220-9.
  24. Coiro, P. et al. (2015) Impaired synaptic development in a maternal immune activation mouse model of neurodevelopmental disorders.
    Brain Behav Immun. pii: S0889-1591(15)00417-1.
  25. Bombeiro, A.L. et al. (2017) Importance of major histocompatibility complex of class I (MHC-I) expression for astroglial reactivity and stability of neural circuits in vitro.
    Neurosci Lett. 647: 97-103.