MHC Class I RT1A antibody | F16-4-4

Mouse anti Rat MHC Class I RT1A

Product Type
Monoclonal Antibody
Clone
F16-4-4
Isotype
IgG1
Specificity
MHC Class I RT1A

Product Code Applications Pack Size List Price Your Price Qty
MCA94R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 recognizes the rat RT1A MHC class I antigen binding to a monomorphic epitope expressed by all rat strains, although strains of haplotype c more weakly.

Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Partially purified rat MHC antigens.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
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Description Mouse IgG1 Negative Control

References for MHC Class I RT1A antibody

  1. Hart, D.N.J. and Fabre, J.W. (1981) Major histocompatibility complex antigens in rat kidney, ureter, and bladder. Localization with monoclonal antibodies and demonstration of Ia-positive dendritic cells
    Transplantation. 31: 318-325.
  2. Hart, D.N.J. and Fabre, J.W. (1981) Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains.
    Transplantation Proceedings. 13: 1329-1322.
  3. Mclellan, A. D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation
    Immunology. 98:171-180.
  4. Tuncel J et al. (2014) Natural polymorphisms in Tap2 influence negative selection and CD4∶CD8 lineage commitment in the rat.
    PLoS Genet. 10 (2): e1004151.
  5. Hart, D.N. & Fabre, J.W. (1981) Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain.
    J Exp Med. 154 (2): 347-61.
  6. Carayon K et al. (2011) Proteolipidic composition of exosomes changes during reticulocyte maturation.
    J Biol Chem. 286 (39): 34426-39.

RRID
AB_323205
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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