MHC Class I RT1A antibody | F16-4-4
Mouse anti Rat MHC class I RT1A antibody, clone F16-4-4 (MCA94R) used for the evaluation of MHC class I expression rat splenocytes by flow cytometry
Image caption:
F16-4-4 recognizes a polymorphic determinant on MHC class Ia. The level of OX18 staining is ~30-fold higher intracellularly than extracellularly, which probably reflects this antibody's affinity for MHC class Ib antigens. (A) DA splenocytes were stained with different concentrations of fluorescent-labelled anti-MHC class Ia and Ib (clone OX18) or anti-MHC class Ia (F16-4-4) antibodies. For detection of intracellular MHC class I, antibody epitopes on the cell surface were first blocked with unlabeled antibody. Cells were then fixed, permeabilized and stained intracellularly with fluorescent labelled antibodies using the same volume as used for extracellular staining. Histograms show samples with comparable mean fluorescent intensity when stained with indicated antibody dilutions (in brackets) extracellularly (red) and intracellularly (blue). (B) F16-4-4 binds a paraformaldehyde (PFA) sensitive epitope on MHC class Ia proteins. Non-permeabilized splenocytes from DA and DA.1IR85 were incubated with saturating concentrations of F16-4-4 (upper panel) or OX18 (lower panel) before (+ PFA) or after (− PFA) incubation of cells in a 4% PFA solution. Note the decreased F16-4-4 staining of PFA- treated cells from DA.1IR85, which suggests that F16-4-4 binds a polymorphic determinant (similar PFA sensitive epitopes were found in DA.1HR83 and DA.1UR83 (data not shown)). Gray histograms represent fluorescence minus one (FMO) controls.
From: Tuncel J, Haag S, Yau ACY, Norin U, Baud A, et al. (2014) Natural Polymorphisms in Tap2 Influence Negative Selection and CD4:CD8 Lineage Commitment in the Rat.
PLoS Genet 10(2): e1004151.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 recognizes the rat RT1A MHC class I antigen binding to a monomorphic epitope expressed by all rat strains, although strains of haplotype c more weakly.
Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 is routinely tested in flow cytometry on rat splenocytes.
Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 is routinely tested in flow cytometry on rat splenocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Partially purified rat MHC antigens.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I RT1A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
References for MHC Class I RT1A antibody
-
Hart, D.N.J. and Fabre, J.W. (1981) Major histocompatibility complex antigens in rat kidney, ureter, and bladder. Localization with monoclonal antibodies and demonstration of Ia-positive dendritic cells
Transplantation. 31: 318-325. -
Hart, D.N.J. and Fabre, J.W. (1981) Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains.
Transplantation Proceedings. 13: 1329-1322. -
Mclellan, A. D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation
Immunology. 98:171-180. -
Tuncel J et al. (2014) Natural polymorphisms in Tap2 influence negative selection and CD4∶CD8 lineage commitment in the rat.
PLoS Genet. 10 (2): e1004151. -
Hart, D.N. & Fabre, J.W. (1981) Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain.
J Exp Med. 154 (2): 347-61. -
Carayon K et al. (2011) Proteolipidic composition of exosomes changes during reticulocyte maturation.
J Biol Chem. 286 (39): 34426-39.
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