MHC Class I RT1A antibody | F16-4-4
Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 is routinely tested in flow cytometry on rat splenocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Partially purified rat MHC antigens.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I RT1A antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/100 | 1/200 | |
Immunohistology - Frozen |
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Product Specific References
References for MHC Class I RT1A antibody
-
Hart, D.N.J. and Fabre, J.W. (1981) Major histocompatibility complex antigens in rat kidney, ureter, and bladder. Localization with monoclonal antibodies and demonstration of Ia-positive dendritic cells
Transplantation. 31: 318-325. -
Hart, D.N.J. and Fabre, J.W. (1981) Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains.
Transplantation Proceedings. 13: 1329-1322. -
Mclellan, A. D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation
Immunology. 98:171-180. -
Tuncel J et al. (2014) Natural polymorphisms in Tap2 influence negative selection and CD4∶CD8 lineage commitment in the rat.
PLoS Genet. 10 (2): e1004151. -
Hart, D.N. & Fabre, J.W. (1981) Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain.
J Exp Med. 154 (2): 347-61. -
Carayon K et al. (2011) Proteolipidic composition of exosomes changes during reticulocyte maturation.
J Biol Chem. 286 (39): 34426-39.
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