Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 recognizes the rat RT1A MHC class I antigen binding to a monomorphic epitope expressed by all rat strains, although strains of haplotype c more weakly.
Mouse anti Rat MHC Class I RT1A antibody, clone F16-4-4 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Partially purified rat MHC antigens.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunised BALB/c mice were fused with cells from the NS-1 mouse myeloma line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class I RT1A antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for MHC Class I RT1A antibody
Hart, D.N.J. and Fabre, J.W. (1981) Major histocompatibility complex antigens in rat kidney, ureter, and bladder. Localization with monoclonal antibodies and demonstration of Ia-positive dendritic cells
Transplantation. 31: 318-325.
Hart, D.N.J. and Fabre, J.W. (1981) Problems in the use of erythrocytes for RT1.A typing studies, probably due to quantitative differences in RT1.A antigen expression in different strains.
Transplantation Proceedings. 13: 1329-1322.
Mclellan, A. D. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation
Tuncel J et al. (2014) Natural polymorphisms in Tap2 influence negative selection and CD4∶CD8 lineage commitment in the rat.
PLoS Genet. 10 (2): e1004151.
Hart, D.N. & Fabre, J.W. (1981) Demonstration and characterization of Ia-positive dendritic cells in the interstitial connective tissues of rat heart and other tissues, but not brain.
J Exp Med. 154 (2): 347-61.
Carayon K et al. (2011) Proteolipidic composition of exosomes changes during reticulocyte maturation.
J Biol Chem. 286 (39): 34426-39.