Glucose Transporter 4 antibody | 1F8


Mouse anti Glucose Transporter 4:FITC
Mouse anti Glucose Transporter 4
- Product Type
- Monoclonal Antibody
- Clone
- 1F8
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
4670-1725F | IF | 0.1 mg |
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4670-1725 | C IF IP P* WB | 50 µg |
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4670-1725GA | C IF IP P WB | 0.1 mg |
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Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetik et al. 2014).
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Monkey Dog Rabbit Human Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- Pack Size: 50 µg
0.09% Sodium Azide (NaN3) Pack Size: 0.1 mg0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Partially purified vesicles containing insulin-responsive glucose transporter 4.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- Pack Size: 50 µgIgG concentration 1 mg/mlPack Size: 0.1 mgIgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19357
- P14672
- P14142
- Entrez Gene
- Slc2a4
- SLC2A4
- Slc2a4
- GO Terms
- GO:0000299 integral to membrane of membrane fraction
- GO:0005887 integral to plasma membrane
- GO:0005360 insulin-responsive hydrogen:glucose symporter activity
- GO:0005792 microsome
- GO:0030140 trans-Golgi network transport vesicle
- GO:0030659 cytoplasmic vesicle membrane
- GO:0048471 perinuclear region of cytoplasm
- GO:0050873 brown fat cell differentiation
- GO:0070062 extracellular vesicular exosome
- GO:0005515 protein binding
- GO:0005975 carbohydrate metabolic process
- GO:0009897 external side of plasma membrane
- GO:0012506 vesicle membrane
- GO:0042593 glucose homeostasis
- GO:0046323 glucose import
- GO:0055056 D-glucose transmembrane transporter activity
- GO:0005625 soluble fraction
- GO:0005771 multivesicular body
- GO:0005905 coated pit
- GO:0032593 insulin-responsive compartment
- GO:0042383 sarcolemma
- GO:0045471 response to ethanol
- Regulatory
- For research purposes only
Applications of Glucose Transporter 4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | Neat | 1/25 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunoprecipitation | |||
Western Blotting |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Secondary Antibodies Available
Product Specific References
References for Glucose Transporter 4 antibody
-
James, D.E. et al. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.
Nature. 333 (6169): 183-5. -
James, D.E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.
Nature. 338 (6210): 83-7. -
Cleasby, M.E. et al. (2003) Programming of rat muscle and fat metabolism by in utero overexposure to glucocorticoids.
Endocrinology. 144 (3): 999-1007. -
Huang, J., et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.
Proc Natl Acad Sci U S A. 98:13084-13089. -
Farese, R.V. et al. (2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
J Clin Invest. 117: 2289-301. -
Grainger, D.L. et al. (2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.
Pflugers Arch. 462: 723-32. -
Minakawa, M. et al. (2011) Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
J Clin Biochem Nutr. 48: 237-44. -
Gillies, R.M. et al. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium.
J Mol Histol. 42: 341-54. -
Aksentijević, D. et al. (2009) Insulin resistance and altered glucose transporter 4 expression in experimental uremia.
Kidney Int. 75: 711-8. -
Imamura, T. et al. (2001) beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.
J Biol Chem. 276 (47): 43663-7. -
Allard, M.F. et al. (2000) Hypertrophied rat hearts are less responsive to the metabolic and functional effects of insulin.
Am J Physiol Endocrinol Metab. 279 (3): E487-93. -
Lalioti, V.S. et al. (2002) The insulin-sensitive glucose transporter, GLUT4, interacts physically with Daxx. Two proteins with capacity to bind Ubc9 and conjugated to SUMO1.
J Biol Chem. 277: 19783-91. -
Spargo, F.J. et al. (2007) Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity.
Am J Physiol Endocrinol Metab. 292: E1631-6. -
Cetik, S. et al. (2014) Expression and Localization of Glucose Transporters in Rodent Submandibular Salivary Glands.
Cell Physiol Biochem. 33: 1149-1161. -
de Laat, M.A. et al. (2015) AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle.
Vet J. 205 (3): 381-6. -
Lee, Y-S. et al. (2015) Honokiol, magnolol, and a combination of both compounds improve glucose metabolism in high-fat diet-induced obese mice
Food Sci Biotech. 24 (4): 1467-74.
Further Reading
-
Berger, J. et al. (1989) Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting.
Nature. 340 (6228): 70-2.
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