Glucose Transporter 4 antibody | 1F8
Mouse anti Rat glucose transporter-4 antibody, clone 1F8 (4670-1725) used for the evaluation of GLUT-4 expression in myotube lysates by western blotting.
Image caption:
Effect of resveratrol on translocation of GLUT4 to plasma membrane and phosphorylation of AMPK and Akt. A: Translocation of GLUT4 to plasma membrane of L6 myotubes which were exposed to 100 μM resveratrol for 15 min. GLUT4 and plasma membrane were detected by western blotting analyses with anti-GLUT4 and anti-Na+/K+ ATPase antibodies. Post-PM and PM indicate post plasma membrane and plasma membrane fractions, respectively. B, C: Effect of resveratrol on phosphorylation of AMPK (B) and Akt (C). L6 myotubes were exposed to resveratrol (10 μM) for indicated time intervals. Western blotting analyses were carried out with anti-AMPK, anti-phospho-AMPK, anti-Akt and anti-phospho-Akt antibodies. D, E: Time-dependent changes of GLUT4 translocation in L6 myotubes at 10 μM (D) or 100 μM (E) resveratrol. L6 myotubes were exposed to resveratrol for indicated time intervals. Plasma membrane fractions of L6 myotubes were subjected to western blotting analyses.
From: Minakawa M, Kawano A, Miura Y, Yagasaki K.
Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
From: J Clin Biochem Nutr. 2011 May;48(3):237-44.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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IF WB ResetMouse anti Glucose Transporter 4
- Product Type
- Monoclonal Antibody
- Clone
- 1F8
- Isotype
- IgG1
- Specificity
- Glucose Transporter 4
Antibody Quality and Performance Guarantee
| Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al. 1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (James et al. 1988). Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetik et al. 2014). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Monkey Dog Rabbit Human Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- 4670-1725GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 4670-1725: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)Pack Size: 50 µg
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Partially purified vesicles containing insulin-responsive glucose transporter 4.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 1.0mg/mlPack Size: 50 µgIgG concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
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| Immunoprecipitation | |
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| Western Blotting | |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Glucose Transporter 4 antibody
-
James, D.E. et al. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.
Nature. 333 (6169): 183-5. -
James, D.E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.
Nature. 338 (6210): 83-7. -
Cleasby, M.E. et al. (2003) Programming of rat muscle and fat metabolism by in utero overexposure to glucocorticoids.
Endocrinology. 144 (3): 999-1007. -
Huang, J., et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.
Proc Natl Acad Sci U S A. 98:13084-13089. -
Farese, R.V. et al. (2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
J Clin Invest. 117: 2289-301. -
Grainger, D.L. et al. (2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.
Pflugers Arch. 462: 723-32. -
Minakawa, M. et al. (2011) Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
J Clin Biochem Nutr. 48: 237-44. -
Gillies, R.M. et al. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium.
J Mol Histol. 42: 341-54.
View The Latest Product References
-
Aksentijević, D. et al. (2009) Insulin resistance and altered glucose transporter 4 expression in experimental uremia.
Kidney Int. 75: 711-8. -
Imamura, T. et al. (2001) beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.
J Biol Chem. 276 (47): 43663-7. -
Allard, M.F. et al. (2000) Hypertrophied rat hearts are less responsive to the metabolic and functional effects of insulin.
Am J Physiol Endocrinol Metab. 279 (3): E487-93. -
Lalioti, V.S. et al. (2002) The insulin-sensitive glucose transporter, GLUT4, interacts physically with Daxx. Two proteins with capacity to bind Ubc9 and conjugated to SUMO1.
J Biol Chem. 277: 19783-91. -
Spargo, F.J. et al. (2007) Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity.
Am J Physiol Endocrinol Metab. 292: E1631-6. -
Cetik, S. et al. (2014) Expression and Localization of Glucose Transporters in Rodent Submandibular Salivary Glands.
Cell Physiol Biochem. 33: 1149-1161. -
de Laat, M.A. et al. (2015) AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle.
Vet J. 205 (3): 381-6. -
Lee, Y-S. et al. (2015) Honokiol, magnolol, and a combination of both compounds improve glucose metabolism in high-fat diet-induced obese mice
Food Sci Biotech. 24 (4): 1467-74. -
Lee, Y. et al. (2012) Fargesin improves lipid and glucose metabolism in 3T3-L1 adipocytes and high-fat diet-induced obese mice
BioFactors. 38 (4): 300-8.
Further Reading
-
Berger, J. et al. (1989) Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting.
Nature. 340 (6228): 70-2.
- Synonyms
- GLUT4
- RRID
- AB_2191547
- UniProt
- P19357
- P14672
- P14142
- Entrez Gene
- Slc2a4
- SLC2A4
- Slc2a4
- GO Terms
- GO:0000299 integral to membrane of membrane fraction
- GO:0005887 integral to plasma membrane
- GO:0005360 insulin-responsive hydrogen:glucose symporter activity
- GO:0005792 microsome
- GO:0030140 trans-Golgi network transport vesicle
- GO:0030659 cytoplasmic vesicle membrane
- GO:0048471 perinuclear region of cytoplasm
- GO:0050873 brown fat cell differentiation
- GO:0070062 extracellular vesicular exosome
- View More GO Terms
- GO:0005515 protein binding
- GO:0005975 carbohydrate metabolic process
- GO:0009897 external side of plasma membrane
- GO:0012506 vesicle membrane
- GO:0042593 glucose homeostasis
- GO:0046323 glucose import
- GO:0055056 D-glucose transmembrane transporter activity
- GO:0005625 soluble fraction
- GO:0005771 multivesicular body
- GO:0005905 coated pit
- GO:0032593 insulin-responsive compartment
- GO:0042383 sarcolemma
- GO:0045471 response to ethanol
4670-1725GA
161442
1705
4670-1725
151877
158821
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