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Glucose Transporter 4 antibody | 1F8

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Anti Glucose Transporter 4 Antibody, clone 1F8 thumbnail image 1 Anti Glucose Transporter 4 Antibody, clone 1F8 thumbnail image 2
Anti Glucose Transporter 4 Antibody, clone 1F8 gallery image 1
HeLa cells stained with Mouse anti Glucose Transporter 4:FITC (4670-1725F) and counterstained with DAPI.
Anti Glucose Transporter 4 Antibody, clone 1F8 gallery image 2
Published customer image:
Mouse anti Rat glucose transporter-4 antibody, clone 1F8 (4670-1725) used for the evaluation of GLUT-4 expression in myotube lysates by western blotting.
Image caption:
Effect of resveratrol on translocation of GLUT4 to plasma membrane and phosphorylation of AMPK and Akt. A: Translocation of GLUT4 to plasma membrane of L6 myotubes which were exposed to 100 μM resveratrol for 15 min. GLUT4 and plasma membrane were detected by western blotting analyses with anti-GLUT4 and anti-Na+/K+ ATPase antibodies. Post-PM and PM indicate post plasma membrane and plasma membrane fractions, respectively. B, C: Effect of resveratrol on phosphorylation of AMPK (B) and Akt (C). L6 myotubes were exposed to resveratrol (10 μM) for indicated time intervals. Western blotting analyses were carried out with anti-AMPK, anti-phospho-AMPK, anti-Akt and anti-phospho-Akt antibodies. D, E: Time-dependent changes of GLUT4 translocation in L6 myotubes at 10 μM (D) or 100 μM (E) resveratrol. L6 myotubes were exposed to resveratrol for indicated time intervals. Plasma membrane fractions of L6 myotubes were subjected to western blotting analyses.

From: Minakawa M, Kawano A, Miura Y, Yagasaki K.
Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
From: J Clin Biochem Nutr. 2011 May;48(3):237-44.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Glucose Transporter 4:FITC

Mouse anti Glucose Transporter 4

Product Type
Monoclonal Antibody
Clone
1F8
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
4670-1725F
Datasheet
IF
SDS
0.1 mg loader

4670-1725
Datasheet
C IF IP P* WB
SDS
50 µg loader

4670-1725GA
Datasheet
C IF IP P WB
SDS
0.1 mg loader

Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al. 1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (James et al. 1988).

Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetik et al. 2014).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Monkey
Dog
Rabbit
Human
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 50 µg
0.09%Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Partially purified vesicles containing insulin-responsive glucose transporter 4.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
Pack Size: 50 µg
IgG concentration 1 mg/ml
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P19357
P14672
P14142
Entrez Gene
Slc2a4
SLC2A4
Slc2a4
GO Terms
GO:0000299 integral to membrane of membrane fraction
GO:0005887 integral to plasma membrane
GO:0005360 insulin-responsive hydrogen:glucose symporter activity
GO:0005792 microsome
GO:0030140 trans-Golgi network transport vesicle
GO:0030659 cytoplasmic vesicle membrane
GO:0048471 perinuclear region of cytoplasm
GO:0050873 brown fat cell differentiation
GO:0070062 extracellular vesicular exosome
GO:0005515 protein binding
GO:0005975 carbohydrate metabolic process
GO:0009897 external side of plasma membrane
GO:0012506 vesicle membrane
GO:0042593 glucose homeostasis
GO:0046323 glucose import
GO:0055056 D-glucose transmembrane transporter activity
GO:0005625 soluble fraction
GO:0005771 multivesicular body
GO:0005905 coated pit
GO:0032593 insulin-responsive compartment
GO:0042383 sarcolemma
GO:0045471 response to ethanol
Regulatory
For research purposes only

Applications of Glucose Transporter 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence Neat 1/25
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Glucose Transporter 4 antibody

  1. James, D.E. et al. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.
    Nature. 333 (6169): 183-5.
  2. James, D.E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.
    Nature. 338 (6210): 83-7.
  3. Cleasby, M.E. et al. (2003) Programming of rat muscle and fat metabolism by in utero overexposure to glucocorticoids.
    Endocrinology. 144 (3): 999-1007.
  4. Huang, J., et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.
    Proc Natl Acad Sci U S A. 98:13084-13089.
  5. Farese, R.V. et al. (2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
    J Clin Invest. 117: 2289-301.
  6. Grainger, D.L. et al. (2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.
    Pflugers Arch. 462: 723-32.
  7. Minakawa, M. et al. (2011) Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
    J Clin Biochem Nutr. 48: 237-44.
  8. Gillies, R.M. et al. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium.
    J Mol Histol. 42: 341-54.
  9. Aksentijević, D. et al. (2009) Insulin resistance and altered glucose transporter 4 expression in experimental uremia.
    Kidney Int. 75: 711-8.
  10. Imamura, T. et al. (2001) beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.
    J Biol Chem. 276 (47): 43663-7.
  11. Allard, M.F. et al. (2000) Hypertrophied rat hearts are less responsive to the metabolic and functional effects of insulin.
    Am J Physiol Endocrinol Metab. 279 (3): E487-93.
  12. Lalioti, V.S. et al. (2002) The insulin-sensitive glucose transporter, GLUT4, interacts physically with Daxx. Two proteins with capacity to bind Ubc9 and conjugated to SUMO1.
    J Biol Chem. 277: 19783-91.
  13. Spargo, F.J. et al. (2007) Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity.
    Am J Physiol Endocrinol Metab. 292: E1631-6.
  14. Cetik, S. et al. (2014) Expression and Localization of Glucose Transporters in Rodent Submandibular Salivary Glands.
    Cell Physiol Biochem. 33: 1149-1161.
  15. de Laat, M.A. et al. (2015) AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle.
    Vet J. 205 (3): 381-6.
  16. Lee, Y-S. et al. (2015) Honokiol, magnolol, and a combination of both compounds improve glucose metabolism in high-fat diet-induced obese mice
    Food Sci Biotech. 24 (4): 1467-74.

Further Reading

  1. Berger, J. et al. (1989) Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting.
    Nature. 340 (6228): 70-2.

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