Glucose Transporter 4 antibody | 1F8

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Anti Glucose Transporter 4 Antibody, clone 1F8 thumbnail image 1

Anti Glucose Transporter 4 Antibody, clone 1F8 thumbnail image 2

Anti Glucose Transporter 4 Antibody, clone 1F8 thumbnail image 3

Anti Glucose Transporter 4 Antibody, clone 1F8 gallery image 1 — 3T3-L1 lysate probed with Mouse anti Rat GLUT4:HRP (**4670-1725P**).

Anti Glucose Transporter 4 Antibody, clone 1F8 gallery image 2 — HeLa cells stained with Mouse anti Glucose Transporter 4:FITC (**4670-1725F**) and counterstained with DAPI.

Anti Glucose Transporter 4 Antibody, clone 1F8 gallery image 3 — **Published customer image:**
Mouse anti Rat glucose transporter-4 antibody, clone 1F8 (**4670-1725**) used for the evaluation of GLUT-4 expression in myotube lysates by western blotting.
**Image caption:**
Effect of resveratrol on translocation of GLUT4 to plasma membrane and phosphorylation of AMPK and Akt. A: Translocation of GLUT4 to plasma membrane of L6 myotubes which were exposed to 100 μM resveratrol for 15 min. GLUT4 and plasma membrane were detected by western blotting analyses with anti-GLUT4 and anti-Na+/K+ ATPase antibodies. Post-PM and PM indicate post plasma membrane and plasma membrane fractions, respectively. B, C: Effect of resveratrol on phosphorylation of AMPK (B) and Akt (C). L6 myotubes were exposed to resveratrol (10 μM) for indicated time intervals. Western blotting analyses were carried out with anti-AMPK, anti-phospho-AMPK, anti-Akt and anti-phospho-Akt antibodies. D, E: Time-dependent changes of GLUT4 translocation in L6 myotubes at 10 μM (D) or 100 μM (E) resveratrol. L6 myotubes were exposed to resveratrol for indicated time intervals. Plasma membrane fractions of L6 myotubes were subjected to western blotting analyses.

From: Minakawa M, Kawano A, Miura Y, Yagasaki K.
Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
From: J Clin Biochem Nutr. 2011 May;48(3):237-44.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Glucose Transporter 4:FITC

Mouse anti Glucose Transporter 4:HRP

Mouse anti Glucose Transporter 4

Product Type: Monoclonal Antibody
Clone: 1F8
Isotype: IgG1

Product Code	Applications	Pack Size
4670-1725F	IF	0.1 mg
4670-1725P	C WB	0.1 mg
4670-1725	C IF IP P* WB	50 µg
4670-1725GA	C IF IP P WB	0.1 mg

Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al. 1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (James et al. 1988).

Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetik et al. 2014).

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Monkey
Dog
Rabbit
Human
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.01% Thiomersal

HRP Stabiliser (BUF052A)

Preservative Stabilisers

Pack Size: 50 µg

0.09%

Sodium Azide (NaN₃)

Pack Size: 0.1 mg

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Partially purified vesicles containing insulin-responsive glucose transporter 4.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Approx. Protein Concentrations

Pack Size: 50 µg

IgG concentration 1 mg/ml

Pack Size: 0.1 mg

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P19357 Related reagents; P14672 Related reagents; P14142 Related reagents
Entrez Gene: Slc2a4 Related reagents; SLC2A4 Related reagents; Slc2a4 Related reagents
GO Terms: GO:0000299 integral to membrane of membrane fraction; GO:0005887 integral to plasma membrane; GO:0005360 insulin-responsive hydrogen:glucose symporter activity; GO:0005792 microsome; GO:0030140 trans-Golgi network transport vesicle; GO:0030659 cytoplasmic vesicle membrane; GO:0048471 perinuclear region of cytoplasm; GO:0050873 brown fat cell differentiation; GO:0070062 extracellular vesicular exosome; GO:0005515 protein binding; GO:0005975 carbohydrate metabolic process; GO:0009897 external side of plasma membrane; GO:0012506 vesicle membrane; GO:0042593 glucose homeostasis; GO:0046323 glucose import; GO:0055056 D-glucose transmembrane transporter activity; GO:0005625 soluble fraction; GO:0005771 multivesicular body; GO:0005905 coated pit; GO:0032593 insulin-responsive compartment; GO:0042383 sarcolemma; GO:0045471 response to ethanol
Regulatory: For research purposes only

Applications of Glucose Transporter 4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence	Neat	1/25
Immunohistology - Frozen
Western Blotting	1/100	1/1000
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹
Immunoprecipitation
Western Blotting

¹ This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Glucose Transporter 4 antibody

James, D.E. et al. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.
Nature. 333 (6169): 183-5.
James, D.E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.
Nature. 338 (6210): 83-7.
Cleasby, M.E. et al. (2003) Programming of rat muscle and fat metabolism by in utero overexposure to glucocorticoids.
Endocrinology. 144 (3): 999-1007.
Huang, J., et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.
Proc Natl Acad Sci U S A. 98:13084-13089.
Farese, R.V. et al. (2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
J Clin Invest. 117: 2289-301.
Grainger, D.L. et al. (2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.
Pflugers Arch. 462: 723-32.
Minakawa, M. et al. (2011) Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells.
J Clin Biochem Nutr. 48: 237-44.
Gillies, R.M. et al. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium.
J Mol Histol. 42: 341-54.
Aksentijević, D. et al. (2009) Insulin resistance and altered glucose transporter 4 expression in experimental uremia.
Kidney Int. 75: 711-8.
Imamura, T. et al. (2001) beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.
J Biol Chem. 276 (47): 43663-7.
Allard, M.F. et al. (2000) Hypertrophied rat hearts are less responsive to the metabolic and functional effects of insulin.
Am J Physiol Endocrinol Metab. 279 (3): E487-93.
Lalioti, V.S. et al. (2002) The insulin-sensitive glucose transporter, GLUT4, interacts physically with Daxx. Two proteins with capacity to bind Ubc9 and conjugated to SUMO1.
J Biol Chem. 277: 19783-91.
Spargo, F.J. et al. (2007) Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity.
Am J Physiol Endocrinol Metab. 292: E1631-6.
Cetik, S. et al. (2014) Expression and Localization of Glucose Transporters in Rodent Submandibular Salivary Glands.
Cell Physiol Biochem. 33: 1149-1161.
de Laat, M.A. et al. (2015) AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle.
Vet J. 205 (3): 381-6.
Lee, Y-S. et al. (2015) Honokiol, magnolol, and a combination of both compounds improve glucose metabolism in high-fat diet-induced obese mice
Food Sci Biotech. 24 (4): 1467-74.

Glucose Transporter 4 antibody | 1F8

Product Details

Storage Information

More Information

Applications of Glucose Transporter 4 antibody

Secondary Antibodies Available

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Glucose Transporter 4 antibody

Further Reading