CD61 antibody | F11

Mouse anti Rat CD61:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price

Mouse anti Rat CD61 antibody, clone F11 recognizes the rat CD61 cell surface antigen, also known as the integrin b3 chain.

CD61 is expressed by platelets, megakaryocytes and osteoclasts.Clone F11 has been shown to inhibit the in vitro attachment of osteoclasts to various proteins including vitronectin, fibrinogen and von Willebrand Factor (Sick et al. 2009).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Bone cell suspension from SD rats.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with the cells of the mouse X63-Ag8 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

References for CD61 antibody

  1. Helfrich, M.H. et al. (1992) Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
    J Bone Miner Res. 7 (3): 345-51.
  2. Shankar, G. et al. (1993) Integrin receptor-mediated mobilisation of intranuclear calcium in rat osteoclasts.
    J Cell Sci. 105 ( Pt 1): 61-8.
  3. Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
    Cell Mol Life Sci. 66: 1271-82.
  4. Wang, Z. et al. (2015) Microparticle levels after arterial injury and NO therapy in diabetes.
    J Surg Res. pii: S0022-4804(15)00867-7

Integrin Beta 3 Chain



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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