CD54 antibody | 1A29
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Mouse anti Rat CD54
- Product Type
- Monoclonal Antibody
|Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a ~90 kDa adhesion molecule belonging to the immunoglobulin superfamily.
CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and plays an important role in various cell-cell interactions in the immune system. Cross-linking of ICAM-1 using clone 1A29 induces calcium signaling (Etienne et al. 1998).
Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of cells including PHA blasts (Tamatani & Miyasaka 1990).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat Ax cells (a HEV derived cell line).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD54 antibody
Etienne, S. et al. (1998) ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells.
J Immunol. 161 (10): 5755-61.
Kawai, T. et al. (1999) Selective diapedesis of Th1 cells induced by endothelial cell RANTES.
J Immunol. 163: 3269-78.
Adamson, P. et al. (1999) Lymphocyte migration through brain endothelial cell monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway.
J Immunol. 162: 2964-73.
Sato, N. et al. (2000) Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury.
Am J Respir Crit Care Med. 161: 1681-8.
Etienne-Manneville, S. et al. (2000) ICAM-1-coupled cytoskeletal rearrangements and transendothelial lymphocyte migration involve intracellular calcium signaling in brain endothelial cell lines.
J Immunol. 165 (6): 3375-83.
Arsenović-Ranin, N. et al. (2000) A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes.
Immunology. 100: 334-44.
Beck-Schimmer, B. et al. (2001) Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells.
Am J Respir Cell Mol Biol. 25: 780-7.
McKechnie, N. M. et al. (2002) Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation.
Invest Ophthalmol Vis Sci. 43:411-8.
View The Latest Product References
Zhu, X. et al. (2003) Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation.
Ann Clin Lab Sci. 33: 216-25.
Deng, H. et al. (2003) Mild hypothermia inhibits inflammation after experimental stroke and brain inflammation.
Stroke. 34: 2495-501.
Ikezumi, Y. et al. (2004) Macrophage-mediated renal injury is dependent on signaling via the JNK pathway.
J Am Soc Nephrol. 15: 1775-84.
Westermann, J. et al. (2005) Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells.
J Immunol. 174: 2517-24.
Westermann, D. et al. (2007) Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.
Diabetes. 56: 1834-41.
Couty, J.P. (2007) PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
J Neurochem. 103: 793-801.
Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 14: 1149-56.
Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
Nephrol Dial Transplant. 25: 2898-908.
Azcutia V et al. (2010) Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo.
PLoS One. 5 (4): e10091.
Choi, J.S. et al. (2011) Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model.
Stroke Res Treat. 2011: 846716.
Li, W. & Klein, S.L. (2012) Seoul virus-infected rat lung endothelial cells and alveolar macrophages differ in their ability to support virus replication and induce regulatory T cell phenotypes.
J Virol. 86 (21): 11845-55.
Gates, D. et al. (2012) Apo J/clusterin expression and secretion: evidence for 15-deoxy-Δ(12,14)-PGJ(2)-dependent mechanism.
Biochim Biophys Acta. 1821 (2): 335-42.
Liu, Y.C. et al. (2013) A biodegradable, sustained-released, prednisolone acetate microfilm drug delivery system effectively prolongs corneal allograft survival in the rat keratoplasty model.
PLoS One. 8 (8): e70419.
Li, Z. et al. (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring.
Mater Sci Eng C Mater Biol Appl. 57: 181-8.
Gautier, S. et al. (2015) PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats.
PPAR Res. 2015: 246329.
Ichihara, Y. et al. (2018) Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure.
Biomaterials. 154: 12-23.
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines. 10 (5): 1021.
- Entrez Gene
- GO Terms
- GO:0001541 ovarian follicle development
- GO:0001975 response to amphetamine
- GO:0016021 integral to membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007569 cell aging
- GO:0008360 regulation of cell shape
- GO:0042493 response to drug
- GO:0010212 response to ionizing radiation
- View More GO Terms
- GO:0010477 response to sulfur dioxide
- GO:0014070 response to organic cyclic compound
- GO:0031669 cellular response to nutrient levels
- GO:0034698 response to gonadotropin stimulus
- GO:0043200 response to amino acid stimulus
- GO:0043498 cell surface binding
- GO:0046688 response to copper ion
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045471 response to ethanol
- GO:0045907 positive regulation of vasoconstriction
- GO:0050900 leukocyte migration
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051926 negative regulation of calcium ion transport
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071312 cellular response to alkaloid
- GO:0071333 cellular response to glucose stimulus
- GO:0071347 cellular response to interleukin-1
- GO:0071356 cellular response to tumor necrosis factor
- GO:0071456 cellular response to hypoxia
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