Antigen Retrieval Buffer, Universal

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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HIS003BPdatasheet pdfdatasheet pdf250 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Intended Use
      Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation is suitable for use with most antibodies and gives excellent preservation of tissue morphology.
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Bio-Rad's Antigen unmasking fluid is supplied as 3x concentrated fluid. Crystals in the fluid will dissolve during heating before use.
    • Storage
      Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Deparaffinize sections and re-hydrate.

      2. Dilute concentrated Antigen unmasking fluid 1:3 with de-ionised water (1 part Antigen unmasking fluid:2 parts water).

      3. Pre-heat retrieval buffer to 92oC *.

      4. Place slides in buffer and incubate 10 minutes at 92oC *.

      5. Allow to cool for 5-10 minutes.

      6. Rinse in distilled water or PBS, proceed with staining.

      * N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.

    Additional Antigen Retrieval Buffer, Universal Formats

    Formats Applications Sizes available
    Antigen Retrieval Buffer, Universal : 3 X Concentrate P 250 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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