CD49f antibody | Mab-5A
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|Mouse anti Rat CD49f antibody, clone Mab-5A recognizes rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively.
Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat oesophageal carcinoma cell line B2T.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Jamasbi, R.J. et al. (1992) A monoclonal antibody produced against a rat esophageal carcinoma cell line reacts with an integrin-like molecule expressed by rat epithelial cells.
Hybridoma. 11 (5): 581-94.
References for CD49f antibody
Paku, S. et al. (2004) 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies.
Hepatology. 39: 1353-61.
Sanchez-Esteban, J. et al. (2006) Integrins beta1, alpha6, and alpha3 contribute to mechanical strain-induced differentiation of fetal lung type II epithelial cells via distinct mechanisms.
Am J Physiol Lung Cell Mol Physiol. 290: L343-50.
D'Souza, R. et el (2009) Disruption of tubulobulbar complex by high intratesticular estrogens leading to failed spermiation.
Endocrinology. 150: 1861-9.
Krause, P. et al. (2010) Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
World J Gastroenterol. 16: 3928-35.
Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 42: 129-36.
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic
adult progenitor cell populations.
Stem Cells Dev. 18: 1389-98.
Koenig, S. et al. (2006) Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration.
Histochem Cell Biol.126: 723-34.
Chen, J. et al. (2009) Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Connect Tissue Res. 50: 294-306.
View The Latest Product References
Patrick, J.C. & Wu, X. (2003) Integrin-mediated preadipocyte adhesion and migration on laminin-1.
Ann Biomed Eng. 31 (5): 505-14.
Kurzrock, E.A. et al. (2008) Label-retaining cells of the bladder: candidate urothelial stem cells.
Am J Physiol Renal Physiol. 294 (6): F1415-21.
Kudo, K.I. et al. (2020) Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses.
Radiat Res. 194 (1): 22-37.
Dufresne, J. et al. (2022) Differential Gene Expression and Hallmarks of Stemness in Epithelial Cells of the Developing Rat Epididymis
Res Sq. 09 Feb [Epub ahead of print].
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