CD49f antibody | Mab-5A

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Anti Rat CD49f Antibody, clone Mab-5A thumbnail image 1

Anti Rat CD49f Antibody, clone Mab-5A thumbnail image 2

Anti Rat CD49f Antibody, clone Mab-5A gallery image 1 — Normal rat kidney epithelial (NRK) cells stained with Alexa Fluor^®647 conjugated Mouse anti Rat CD49f (**MCA2034A647**).

Anti Rat CD49f Antibody, clone Mab-5A gallery image 2 — Normal rat kidney epithelial (NRK) cells stained with FITC conjugated Mouse anti Rat CD49f (**MCA2034F**).

Mouse anti Rat CD49f:Alexa Fluor® 647

Mouse anti Rat CD49f:FITC

Mouse anti Rat CD49f

Mouse anti Rat CD49f:RPE

Product Type: Monoclonal Antibody
Clone: Mab-5A
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2034A647	F	100 Tests/1ml
MCA2034F	F	0.1 mg
MCA2034G	C E F IF	0.25 mg
MCA2034PE	F	100 Tests

Mouse anti Rat CD49f antibody, clone Mab-5A recognizes rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively.

Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts.

Product Details

Target Species

Rat

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Immunogen

Rat oesophageal carcinoma cell line B2T.

Approx. Protein Concentrations

IgG concentration 0.05mg/ml

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of despatch.

More Information

Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD49f antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry	Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA1209A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA1209F	0.1 mg	F
Mouse IgG1 Negative Control	MCA1209	0.1 mg	F
Mouse IgG1 Negative Control:RPE	MCA1209PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Histar Detection System	STAR3000B	150 Tests	C P

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD49f antibody

Jamasbi, R.J. et al. (1992) A monoclonal antibody produced against a rat esophageal carcinoma cell line reacts with an integrin-like molecule expressed by rat epithelial cells.
Hybridoma. 11 (5): 581-94.
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18: 1389-98.
D'Souza, R. et el (2009) Disruption of tubulobulbar complex by high intratesticular estrogens leading to failed spermiation.
Endocrinology. 150: 1861-9.
Krause, P. et al. (2010) Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
World J Gastroenterol. 16: 3928-35.
Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 42: 129-36.
Sanchez-Esteban, J. et al. (2006) Integrins beta1, alpha6, and alpha3 contribute to mechanical strain-induced differentiation of fetal lung type II epithelial cells via distinct mechanisms.
Am J Physiol Lung Cell Mol Physiol. 290: L343-50.
Paku, S. et al. (2004) 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies.
Hepatology. 39: 1353-61.
Chen, J. et al. (2009) Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Connect Tissue Res. 50: 294-306.
Koenig, S. et al. (2006) Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration.
Histochem Cell Biol.126: 723-34.

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