CD49f antibody | Mab-5A
Mouse anti Rat CD49f antibody, clone Mab-5A (MCA2034G) used for immunomagnetic separation of rat epididymal cells and AlexaFluor647 conjugated Mab-5A used for assessment of CD49f expression on epididymal cells by flow cytometry.
Image caption:
Selection of CD49f+ cells from epididymides of 7-day old rats. (A) Immunouorescent staining of CD49f (red) in caput (a) and cauda (b) epididymides of 7-day old rats. CD49f was present at the basal and basolateral regions of the epididymal epithelium. The negative control, in which sections were incubated in absence of primary antibody, is shown in (c). Scale bar = 10 μm. (B) Magnetic separation of CD49f+ and CD49f- cells from epididymides of 7-day old rats. Organoids were present only in CD49f+ cells. Scale bar = 200μm. III. Analysis of CD49f+high and CD49f+low cells in the epididymis of young rats. Cells from epididymides of 10-day old rats were incubated on ice for 30 min with a mouse monoclonal anti-rat Cd49f antibody conjugated with Alexa Fluor®647 and subjected to flow cytometric analyses. (B) Histogram showing the fluorescence of the cells in the absence of the CD49f-Alexa Fluor 647 conjugated antibody. (C) Histogram showing the uorescence of the cells incubated with the CD49f-Alexa Fluor 647 conjugated antibody.
From: Dufresne J, Gregory M, Pinel L, et al.
Differential Gene Expression and Hallmarks of Stemness in Epithelial Cells of the Developing Rat Epididymis.
Preprint, Res Sq. 09 Feb 2022, [Epub ahead of print].
doi: 10.21203/rs.3.rs-1287069/v1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IM ResetMouse anti Rat CD49f
- Product Type
- Monoclonal Antibody
- Clone
- Mab-5A
- Isotype
- IgG1
- Specificity
- CD49f
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD49f antibody, clone Mab-5A recognizes rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively. Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rat oesophageal carcinoma cell line B2T.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Jamasbi, R.J. et al. (1992) A monoclonal antibody produced against a rat esophageal carcinoma cell line reacts with an integrin-like molecule expressed by rat epithelial cells.
Hybridoma. 11 (5): 581-94.
References for CD49f antibody
-
Patrick, J.C. & Wu, X. (2003) Integrin-mediated preadipocyte adhesion and migration on laminin-1.
Ann Biomed Eng. 31 (5): 505-14. -
Paku, S. et al. (2004) 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies.
Hepatology. 39: 1353-61. -
Koenig, S. et al. (2006) Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration.
Histochem Cell Biol.126: 723-34. -
Sanchez-Esteban, J. et al. (2006) Integrins beta1, alpha6, and alpha3 contribute to mechanical strain-induced differentiation of fetal lung type II epithelial cells via distinct mechanisms.
Am J Physiol Lung Cell Mol Physiol. 290: L343-50. -
Kurzrock, E.A. et al. (2008) Label-retaining cells of the bladder: candidate urothelial stem cells.
Am J Physiol Renal Physiol. 294 (6): F1415-21. -
Chen, J. et al. (2009) Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
Connect Tissue Res. 50: 294-306. -
Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 42: 129-36. -
D'Souza, R. et el (2009) Disruption of tubulobulbar complex by high intratesticular estrogens leading to failed spermiation.
Endocrinology. 150: 1861-9.
View The Latest Product References
-
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18: 1389-98. -
Krause, P. et al. (2010) Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
World J Gastroenterol. 16: 3928-35. -
Kudo, K.I. et al. (2020) Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses.
Radiat Res. 194 (1): 22-37. -
Dufresne, J. et al. (2022) Differential gene expression and hallmarks of stemness in epithelial cells of the developing rat epididymis.
Cell Tissue Res. 389 (2): 327-49.
- Synonyms
- Integrin Alpha 6 Chain
- VLA-6
- RRID
- AB_10975483
MCA2034G
0610
153353
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