CD49f antibody | Mab-5A

Mouse anti Rat CD49f

Product Type
Monoclonal Antibody
Clone
Mab-5A
Isotype
IgG1
Specificity
CD49f

Product Code Applications Pack Size List Price Your Price Qty
MCA2034G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD49f antibody, clone Mab-5A recognizes rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively.

Mouse anti Rat CD49f antibody, clone Mab-5A does not react with rat fibroblasts.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat oesophageal carcinoma cell line B2T.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
List Price Your Price
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Description Mouse IgG1 Negative Control

Source Reference

  1. Jamasbi, R.J. et al. (1992) A monoclonal antibody produced against a rat esophageal carcinoma cell line reacts with an integrin-like molecule expressed by rat epithelial cells.
    Hybridoma. 11 (5): 581-94.

References for CD49f antibody

  1. Paku, S. et al. (2004) 2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: confocal and electron microscopic studies.
    Hepatology. 39: 1353-61.
  2. Sanchez-Esteban, J. et al. (2006) Integrins beta1, alpha6, and alpha3 contribute to mechanical strain-induced differentiation of fetal lung type II epithelial cells via distinct mechanisms.
    Am J Physiol Lung Cell Mol Physiol. 290: L343-50.
  3. D'Souza, R. et el (2009) Disruption of tubulobulbar complex by high intratesticular estrogens leading to failed spermiation.
    Endocrinology. 150: 1861-9.
  4. Krause, P. et al. (2010) Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
    World J Gastroenterol. 16: 3928-35.
  5. Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
    Acta Histochem Cytochem. 42: 129-36.
  6. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18: 1389-98.
  7. Koenig, S. et al. (2006) Zonal hierarchy of differentiation markers and nestin expression during oval cell mediated rat liver regeneration.
    Histochem Cell Biol.126: 723-34.
  8. Chen, J. et al. (2009) Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.
    Connect Tissue Res. 50: 294-306.
  9. View The Latest Product References
  10. Patrick, J.C. & Wu, X. (2003) Integrin-mediated preadipocyte adhesion and migration on laminin-1.
    Ann Biomed Eng. 31 (5): 505-14.
  11. Kurzrock, E.A. et al. (2008) Label-retaining cells of the bladder: candidate urothelial stem cells.
    Am J Physiol Renal Physiol. 294 (6): F1415-21.
  12. Kudo, K.I. et al. (2020) Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses.
    Radiat Res. 194 (1): 22-37.
  13. Dufresne, J. et al. (2022) Differential Gene Expression and Hallmarks of Stemness in Epithelial Cells of the Developing Rat Epididymis
    Res Sq. 09 Feb [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Synonyms
Integrin Alpha 6 Chain
VLA-6
RRID
AB_10975483
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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