CD45RA antibody | OX-33

Mouse anti Rat CD45RA:FITC

Product Type
Monoclonal Antibody
Clone
OX-33
Isotype
IgG1
Specificity
CD45RA
Region
(B CELLS ONLY)

Product Code Applications Pack Size List Price Your Price Qty
MCA340FT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987).

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Purified Rat spleen L-CA
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
This antibody does not always give a clear separation between positives and negatives in some cell preparations. If users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent MCA340PE which generally exhibits a peak of higher mean fluorescence.

Use 10ul of the suggested working dilutions to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD45RA antibody

  1. Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 15 (2): 168-73.
  2. Barclay, A.N. et al. (1987) Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus.
    EMBO J. 6:1259-1264.
  3. Barclay, A.N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42:593-600.
  4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  5. McCall, M.N. et al. (1992) Expression of soluble isoforms of rat CD45. Analysis by electron microscopy and use in epitope mapping of anti-CD45R monoclonal antibodies.
    Immunology. 76: 310-7.
  6. Cho, W.S. et al. (2012) NiO and Co3O4 nanoparticles induce lung DTH-like responses and alveolar lipoproteinosis.
    Eur Respir J. 39 (3): 546-57.
  7. Williamson Lauren L. et al. (2015) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection
    Brain, Behavior, and Immunity. pii: S0889-1591(15)00240-8.
  8. Pilatz, A. et al. (2015) Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up.
    Andrologia. 47 (2): 160-7.
  9. View The Latest Product References
  10. Stefanski, V. et al. (2013) Differential effect of severe and moderate social stress on blood immune and endocrine measures and susceptibility to collagen type II arthritis in male rats.
    Brain Behav Immun. 29: 156-65.
  11. Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
    Cell Mol Immunol. 9 (2): 163-74.
  12. Okamura, T. et al. (2013) Phenotypic Characterization of LEA Rat: A New Rat Model of Nonobese Type 2 Diabetes.
    J Diabetes Res. 2013: 986462.
  13. Denecke, C. et al. (2013) Synergistic effects of prolonged warm ischemia and donor age on the immune response following donation after cardiac death kidney transplantation.
    Surgery. 153 (2): 249-61.
  14. Marolda, R. et al. (2013) Differential targeting of immune-cells by Pixantrone in experimental myasthenia gravis.
    J Neuroimmunol. 258 (1-2): 41-50.
  15. Han, X. et al. (2013) Porphyromonas gingivalis infection-associated periodontal bone resorption is dependent on receptor activator of NF-κB ligand.
    Infect Immun. 81 (5): 1502-9.
  16. Pongratz, G. et al. (2015) A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
    Immun Ageing. 12: 12.
  17. Lu, J.H. et al. (2015) GABAergic neurons in cerebellar interposed nucleus modulate cellular and humoral immunity via hypothalamic and sympathetic pathways.
    J Neuroimmunol. 283: 30-8.
  18. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  19. Dabrowska, S. et al. (2019) Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
    J Neuroinflammation. 16 (1): 216.
  20. Ogawa, B. et al. (2019) Strain differences in histopathological features of lymphoid tissues of SD and F344 rats in a T cell-dependent antibody response assay of cyclophosphamide.
    J Toxicol Pathol. 32 (3): 143-54.
  21. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
    Biomedicines. 10 (5): 1021.

Flow Cytometry

Immunofluorescence

RRID
AB_322582
UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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