CD45 antibody | OX-1

Product Code Applications Pack Size List Price Your Price Qty
MCA43G
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C * F IF 1 mg loader
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loader
MCA43GA
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C * F IF 0.1 mg loader
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MCA43R
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C * F IF 0.25 mg loader
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Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.

Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.

CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

This product is routinely tested in flow cytometry on rat splenocytes

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD45 antibody

  1. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42: 170-6.
  2. Kurozumi, K. et al. (2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.
    J Natl Cancer Inst. 99: 1768-81.
  3. Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
    Eur J Immunol. 9 (2): 155-9.
  4. Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 15 (2): 168-73.
  5. Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
    Clin Exp Immunol. 99 (2): 283-8.
  6. Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
    J Immunol. 166 (6): 4185-94.
  7. Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
    Int Immunol. 12 (2): 169-76.
  8. Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
    Eur J Immunol. 8 (12): 832-9.
  9. View The Latest Product References
  10. Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
    J Immunol. 163 (1): 71-6.
  11. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209 (1-2): 16-25.
  12. Schupp, N. et al. (2011) Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
    FASEB J. 25 (3): 968-78.
  13. Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
    Am J Pathol. 158: 407-17.
  14. Jeong, H.K. et al (2010) Inflammatory responses are not sufficient to cause delayed neuronal death in ATP-induced acute brain injury.
    PLoS One. 5: e13756.
  15. Leonardo, C.C. et al. (2009) Inhibition of gelatinase activity reduces neural injury in an ex vivo model of hypoxia-ischemia.
    Neuroscience. 160: 755-66.
  16. Markusic, D.M. et al. (2010) Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.
    PLoS One. 5: e9974.
  17. Vaschetto, R. et al. (2010) Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
    Crit Care. 14: R45.
  18. Ladhoff, J. et al. (2010) Immune privilege of endothelial cells differentiated from endothelial progenitor cells.
    Cardiovasc Res. 88: 121-9.
  19. Yao, Y. et al. (2016) Alendronate Attenuates Spinal Microglial Activation and Neuropathic Pain.
    J Pain. 17 (8): 889-903.
  20. Wang, C. et al. (2015) Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD.
    Int Braz J Urol. 41 (4): 764-72.
  21. Tanner, D.C. et al. (2015) cFLIP is critical for oligodendrocyte protection from inflammation.
    Cell Death Differ. 22 (9): 1489-501.
  22. Runesson, E. et al. (2015) Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
    BMC Musculoskelet Disord. 16: 212.
  23. Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
    J Neuroinflammation. 16 (1): 93.
  24. Pilipović, I. et al. (2019) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
    Neurobiol Dis. Nov 2 [Epub ahead of print].
  25. Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
    Stem Cells Dev. 27 (16): 1109-24.
  26. Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
    Toxicol Sci. kfz172.
  27. Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
    Exp Neurol. 336: 113531.
  28. Elabi, O.F. et al. (2021) L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
    Int J Mol Sci. 22(22):12346.
  29. Hou, Y. et al. (2021) Pseudoginsenoside-F11 promotes functional recovery after transient cerebral ischemia by regulating the microglia/macrophage polarization in rats.
    Int Immunopharmacol. 99: 107896.
  30. Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
    PLoS One. 17 (8): e0272697.

Immunofluorescence

Synonyms
LCA
RRID
AB_321738
UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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