CD45 antibody | OX-1
Mouse anti Rat CD45 antibody, clone OX-1 (MCA43R) used for illustration of leukocytes in rat brain by immunohistofluorescence.
Image caption:
Infiltration of monocytes in ATP-injected SNpc.
Sections were obtained 2 d after ATP injection, and double-labeled with Iba-1 and CD45 antibodies. Round Iba-1+ cells were CD45+ (arrows) whereas ramified Iba-1+ cells were CD45- (arrowheads).Scale Bar =50μm (B).
From: Jeong H-K, Ji K-m, Kim B, Kim J, Jou I, Joe E.H. (2010)
Inflammatory Responses Are Not Sufficient to Cause Delayed Neuronal Death in ATP-Induced Acute Brain Injury.
PLoS ONE 5(10): e13756.
RPE-AlexaFluor®750 conjugated Mouse anti Rat CD45 antibody, clone OX-1 (MCA43P750) used for the identification of macrophage populations by flow cytometry.
Image caption:
Flow cytometry performed seven days post-injury. Activated macrophages were distinguished by their expression of CD11b and CD45 (a). No difference was observed in the number of CD11b+CD45HIGH macrophages between treatment groups (b). *P <0.05 (Tukey's Test); error bars represent ± SEM; n = 3 for Controls and 4 for all other groups.
From: Hellenbrand DJ, Reichl KA, Travis BJ, Filipp ME, Khalil AS, Pulito DJ, Gavigan AV, Maginot ER, Arnold MT, Adler AG, Murphy WL, Hanna AS.
Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
J Neuroinflammation. 2019 Apr 30;16(1):93.
doi: 10.1186/s12974-019-1479-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD45, clone OX-1 (MCA43G) used to label microglia in rat brain by immunohistochemistry on formalin fixed cryostections.
Image caption:
Quantification of CD45+ leukocytes in the striatum of the grafted rats. (C, D) Bright field images showing CD45+ cells density and morphology around the graft and their quantification respectively in the striatum of the rats received either saline, exendin-4 or liraglutide and ± LD. ** p <0.01, *** p <0.001, **** p <0.0001. Scale bar (A,C) = 100 μm.
From:Elabi OF, Davies JS, Lane EL.
L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
Int J Mol Sci. 2021 Nov 16;22(22):12346.
doi: 10.3390/ijms222212346.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-phycoerythrin conjugated Mouse anti Rat CD45 antibody, clone OX-1 (MCA43PE) used to characterise rat tendon stem/progenitor cells (TSPCs) by flow cytometry.
Image caption:
Isolation and Characterization of TSPCs.
(C) Flow cytometry analysis of the expression of cell surface makers related to stem cells and hematopoietic cells.
From: Yang Q, Li J, Su W, Yu L, Li T, Wang Y, Zhang K, Wu Y, Wang L.
Electrospun aligned poly(ε-caprolactone) nanofiber yarns guiding 3D organization of tendon stem/progenitor cells in tenogenic differentiation and tendon repair.
Front Bioeng Biotechnol. 2022 Aug 30;10:960694.
doi: 10.3389/fbioe.2022.960694.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD45
- Product Type
- Monoclonal Antibody
- Clone
- OX-1
- Isotype
- IgG1
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This product is routinely tested in flow cytometry on rat splenocytes |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA43G, MCA43GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA43R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD45 antibody
-
Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
Eur J Immunol. 8 (12): 832-9. -
Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Eur J Immunol. 9 (2): 155-9. -
Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73. -
Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
Clin Exp Immunol. 99 (2): 283-8. -
Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
J Immunol. 163 (1): 71-6. -
Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
Int Immunol. 12 (2): 169-76. -
Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
Am J Pathol. 158: 407-17. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6.
View The Latest Product References
-
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Kurozumi, K. et al. (2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.
J Natl Cancer Inst. 99: 1768-81. -
Leonardo, C.C. et al. (2009) Inhibition of gelatinase activity reduces neural injury in an ex vivo model of hypoxia-ischemia.
Neuroscience. 160: 755-66. -
Vaschetto, R. et al. (2010) Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway
Crit Care. 14: R45. -
Ladhoff, J. et al. (2010) Immune privilege of endothelial cells differentiated from endothelial progenitor cells.
Cardiovasc Res. 88: 121-9. -
Jeong, H.K. et al (2010) Inflammatory responses are not sufficient to cause delayed neuronal death in ATP-induced acute brain injury.
PLoS One. 5: e13756. -
Schupp, N. et al. (2011) Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
FASEB J. 25 (3): 968-78. -
Markusic, D.M. et al. (2010) Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.
PLoS One. 5: e9974. -
Runesson, E. et al. (2015) Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing.
BMC Musculoskelet Disord. 16: 212. -
Tanner, D.C. et al. (2015) cFLIP is critical for oligodendrocyte protection from inflammation.
Cell Death Differ. 22 (9): 1489-501. -
Wang, C. et al. (2015) Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD.
Int Braz J Urol. 41 (4): 764-72. -
Yao, Y. et al. (2016) Alendronate Attenuates Spinal Microglial Activation and Neuropathic Pain.
J Pain. 17 (8): 889-903. -
Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
Stem Cells Dev. 27 (16): 1109-24. -
Porwal, K. et al. (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
Toxicol Sci. kfz172. -
Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
J Neuroinflammation. 16 (1): 93. -
Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
Regen Ther. 14: 1-10. -
Pilipović, I. et al. (2020) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
Neurobiol Dis. 134: 104665. -
Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
Exp Neurol. 336: 113531. -
Elabi, O.F. et al. (2021) L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
Int J Mol Sci. 22(22):12346. -
Hou, Y. et al. (2021) Pseudoginsenoside-F11 promotes functional recovery after transient cerebral ischemia by regulating the microglia/macrophage polarization in rats.
Int Immunopharmacol. 99: 107896. -
Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
PLoS One. 17 (8): e0272697. -
Yang, Q. et al. (2022) Electrospun aligned poly(ε-caprolactone) nanofiber yarns guiding 3D organization of tendon stem/progenitor cells in tenogenic differentiation and tendon repair.
Front Bioeng Biotechnol. 10: 960694. -
Wu, Y. et al. (2018) Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.
J Neurosci Res. 96 (7): 1265-76. -
Midavaine, É. et al. (2024) Discovery of a CCR2-targeting pepducin therapy for chronic pain.
Pharmacol Res. : 107242. -
Rendra, E. et al. (2024) Clinical-grade human skin-derived ABCB5+ mesenchymal stromal cells exert anti-apoptotic and anti-inflammatory effects in vitro and modulate mRNA expression in a cisplatin-induced kidney injury murine model.
Front Immunol. 14: 1228928.
- Synonyms
- LCA
- RRID
- AB_321738
- UniProt
- P04157
- Entrez Gene
- Ptprc
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- View More GO Terms
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
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