CD43 antibody | W3/13

Mouse anti Rat CD43

Product Type
Monoclonal Antibody
Clone
W3/13
Isotype
IgG1
Specificity
CD43

Product Code Applications Pack Size List Price Your Price Qty
MCA54G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 1 mg loader
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loader
MCA54GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 0.1 mg loader
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loader
MCA54R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)

Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 Pack Size: 1 mg, 0.1 mg
1/25 Pack Size: 0.25 mg
1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
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Description Mouse IgG1 Negative Control

References for CD43 antibody

  1. Brown, W.R.A. et al. (1981) Identification of a glycophorin-like molecule at the cell surface of rat thymocytes.
    Nature. 289: 456-460.
  2. Barclay, A. N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42: 593-600
  3. Jung, S. et al. (1994) Therapeutic effect of transforming growth factor-beta 2 on actively induced EAN but not adoptive transfer EAN
    Immunology. 83: 545-551.
  4. Bataller, R. et al. (2003) Prolonged infusion of angiotensin II into normal rats induces stellate cell activation and proinflammatory events in liver.
    Am J Physiol Gastrointest Liver Physiol. 285: G642-651
  5. Yamanaka, Y. et al. (2011) Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers.
    Int Endod J. 44: 669-75.
  6. Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
    J Neuroimmunol. 163: 185-9.
  7. Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
    J Comp Neurol. 487: 166-75.
  8. Zhang, Z. et al. (2008) FTY720 ameliorates experimental autoimmune neuritis by inhibition of lymphocyte and monocyte infiltration into peripheral nerves.
    Exp Neurol. 210: 681-90.
  9. View The Latest Product References
  10. Xu, K. et al. (2016) Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
    Diagn Pathol. 11 (1): 72.
  11. Dort, J. et al. (2016) Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
    Biomed Res Int. 2016: 5214561.
  12. Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
    Neurol Sci. 37 (2): 199-203.
  13. Rice, E.K. et al. (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II.
    Kidney Int. 63 (4): 1265-75.
  14. Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
    Muscle Nerve. 48 (3): 403-14.
  15. Dort, J. et al. (2012) Beneficial effects of cod protein on skeletal muscle repair following injury.
    Appl Physiol Nutr Metab. 37 (3): 489-98.
  16. Ornellas, F.M. et al. (2019) Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model.
    Sci Rep. 9 (1): 19604.
  17. Grad, E. et al. (2019) Monocyte Modulation by Liposomal Alendronate Improves Cardiac Healing in a Rat Model of Myocardial Infarction
    Regenerative Engineering and Translational Medicine. 5 (3): 280-9.
  18. Kami, K. et al. (2019) Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat.
    J Histochem Cytochem. 67 (11): 791-9.
  19. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.

Further Reading

  1. Forbes, R. D. C. et al. (1983) Leukocyte subsets in first-set rat cardiac allograft rejection. A serial immunohistologic study using monoclonal antibodies
    Transplantation. 36: 681-686

Synonyms
Leukosialin
RRID
AB_2286557
UniProt
P13838
Entrez Gene
Spn
GO Terms
GO:0002544 chronic inflammatory response
GO:0016021 integral to membrane
GO:0005902 microvillus
GO:0009986 cell surface
GO:0019904 protein domain specific binding
GO:0032154 cleavage furrow
GO:0042117 monocyte activation
GO:0071594 thymocyte aggregation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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