CD43 antibody | W3/13
Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) used to identify Lymphocytes (a + b) in rat brain following traumatic brain injury using immunohistochemistry. (c + d) Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar = 100 μm for (a, c) and d; 50 μm for (b)
From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.
Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmebrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)
Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 0.1 mg, 1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P13838 Related reagents
- Entrez Gene
- Spn Related reagents
- GO Terms
- GO:0002544 chronic inflammatory response
- GO:0016021 integral to membrane
- GO:0005902 microvillus
- GO:0009986 cell surface
- GO:0019904 protein domain specific binding
- GO:0032154 cleavage furrow
- GO:0042117 monocyte activation
- GO:0071594 thymocyte aggregation
- Regulatory
- For research purposes only
Applications of CD43 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 Pack Size: 0.1 mg, 1 mg 1/25 Pack Size: 0.25 mg |
1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Flow Cytometry | Neat |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:RPE | MCA1209PE | 100 Tests | F |
Product Specific References
References for CD43 antibody
-
Brown, W.R.A. et al. (1981) Identification of a glycophorin-like molecule at the cell surface of rat thymocytes.
Nature. 289: 456-460. -
Barclay, A. N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42: 593-600 -
Jung, S. et al. (1994) Therapeutic effect of transforming growth factor-beta 2 on actively induced EAN but not adoptive transfer EAN
Immunology. 83: 545-551. -
Bataller, R. et al. (2003) Prolonged infusion of angiotensin II into normal rats induces stellate cell activation and proinflammatory events in liver.
Am J Physiol Gastrointest Liver Physiol. 285: G642-651 -
Yamanaka, Y. et al. (2011) Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers.
Int Endod J. 44: 669-75. -
Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
J Neuroimmunol. 163: 185-9. -
Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
J Comp Neurol. 487: 166-75. -
Zhang, Z. et al. (2008) FTY720 ameliorates experimental autoimmune neuritis by inhibition of lymphocyte and monocyte infiltration into peripheral nerves.
Exp Neurol. 210: 681-90. -
Xu, K. et al. (2016) Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 11 (1): 72. -
Dort, J. et al. (2016) Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
Biomed Res Int. 2016: 5214561. -
Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
Neurol Sci. 37 (2): 199-203. -
Rice, E.K. et al. (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II.
Kidney Int. 63 (4): 1265-75.
