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CD43 antibody | W3/13

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Anti Rat CD43 Antibody, clone W3/13 thumbnail image 1 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 2 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 3 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 4 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 5 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 6 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 7 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 8 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 9 Anti Rat CD43 Antibody, clone W3/13 thumbnail image 10
Anti Rat CD43 Antibody, clone W3/13 gallery image 1
Figure A. RPE conjugated Mouse anti Rat CD45Ra (MCA340PE) and FITC conjugated Mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated Mouse anti Rat CD45Ra (MCA340PE) and FITC conjugated Mouse anti Rat CD43 (MCA54F). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Rat CD43 Antibody, clone W3/13 gallery image 2
Figure A. RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE) and FITC conjugated Mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE) and FITC conjugated Mouse anti Rat CD43 (MCA54F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD43 Antibody, clone W3/13 gallery image 3
Figure A. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD43 Antibody, clone W3/13 gallery image 4
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54GA) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. Low power
Anti Rat CD43 Antibody, clone W3/13 gallery image 5
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54GA) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. Med power
Anti Rat CD43 Antibody, clone W3/13 gallery image 6
Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54GA) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. High power
Anti Rat CD43 Antibody, clone W3/13 gallery image 7
Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54G) in red and Mouse anti Rat CD4 (MCA55G) in green. Merged image is on the right. Low power
Anti Rat CD43 Antibody, clone W3/13 gallery image 8
Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54G) in red and Mouse anti Rat CD4 (MCA55G) in green. Merged image is on the right. Medium power
Anti Rat CD43 Antibody, clone W3/13 gallery image 9
Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54G) in red and Mouse anti Rat CD4 (MCA55G) in green. Merged image is on the right. High power
Anti Rat CD43 Antibody, clone W3/13 gallery image 10
Published customer image:
Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) used to identify Lymphocytes (a + b) in rat brain following traumatic brain injury using immunohistochemistry. (c + d) Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar = 100 μm for (a, c) and d; 50 μm for (b)

From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.

Mouse anti Rat CD43:FITC

Mouse anti Rat CD43

Mouse anti Rat CD43:RPE

Product Type
Monoclonal Antibody
Clone
W3/13
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA54FT
Datasheet
F
SDS
0.1 mg loader

MCA54G
Datasheet
C F P*
SDS
1 mg loader

MCA54GA
Datasheet
C F P*
SDS
0.1 mg loader

MCA54R
Datasheet
C F P*
SDS
0.25 mg loader

MCA54PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)

Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P13838
Entrez Gene
Spn
GO Terms
GO:0002544 chronic inflammatory response
GO:0016021 integral to membrane
GO:0005902 microvillus
GO:0009986 cell surface
GO:0019904 protein domain specific binding
GO:0032154 cleavage furrow
GO:0042117 monocyte activation
GO:0071594 thymocyte aggregation
Regulatory
For research purposes only

Applications of CD43 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 Pack Size: 1 mg, 0.1 mg
1/25 Pack Size: 0.25 mg		 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1
Flow Cytometry Neat
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader

Product Specific References

References for CD43 antibody

  1. Brown, W.R.A. et al. (1981) Identification of a glycophorin-like molecule at the cell surface of rat thymocytes.
    Nature. 289: 456-460.
  2. Barclay, A. N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42: 593-600
  3. Jung, S. et al. (1994) Therapeutic effect of transforming growth factor-beta 2 on actively induced EAN but not adoptive transfer EAN
    Immunology. 83: 545-551.
  4. Bataller, R. et al. (2003) Prolonged infusion of angiotensin II into normal rats induces stellate cell activation and proinflammatory events in liver.
    Am J Physiol Gastrointest Liver Physiol. 285: G642-651
  5. Yamanaka, Y. et al. (2011) Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers.
    Int Endod J. 44: 669-75.
  6. Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
    J Neuroimmunol. 163: 185-9.
  7. Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
    J Comp Neurol. 487: 166-75.
  8. Zhang, Z. et al. (2008) FTY720 ameliorates experimental autoimmune neuritis by inhibition of lymphocyte and monocyte infiltration into peripheral nerves.
    Exp Neurol. 210: 681-90.
  9. Xu, K. et al. (2016) Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
    Diagn Pathol. 11 (1): 72.
  10. Dort, J. et al. (2016) Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
    Biomed Res Int. 2016: 5214561.
  11. Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
    Neurol Sci. 37 (2): 199-203.
  12. Rice, E.K. et al. (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II.
    Kidney Int. 63 (4): 1265-75.
  13. Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
    Muscle Nerve. 48 (3): 403-14.
  14. Dort, J. et al. (2012) Beneficial effects of cod protein on skeletal muscle repair following injury.
    Appl Physiol Nutr Metab. 37 (3): 489-98.
  15. Ornellas, F.M. et al. (2019) Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model.
    Sci Rep. 9 (1): 19604.
  16. Grad, E. et al. (2019) Monocyte Modulation by Liposomal Alendronate Improves Cardiac Healing in a Rat Model of Myocardial Infarction
    Regenerative Engineering and Translational Medicine. 5 (3): 280-9.
  17. Kami, K. et al. (2019) Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat.
    J Histochem Cytochem. 67 (11): 791-9.

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