CD43 antibody | W3/13
Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) used to identify Lymphocytes (a + b) in rat brain following traumatic brain injury using immunohistochemistry. (c + d) Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar = 100 μm for (a, c) and d; 50 μm for (b)
From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.
Mouse anti Rat CD43 antibody, clone W3/13 (MCA54R) used to label T lymphocytes in Rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Paracortex of the rat popliteal lymph node stained for the presence of T lymphocytes (CD43+, red). (A) The node of the control animal receiving 0.9% NaCl (days 1–7, node evaluation on day 8). (B) The node after initial massive S. epidermidis infection (days 1–7) with early node evaluation (day 8). (C) The node after secondary S. epidermidis infection (day 28, node evaluation on day 29). Magnification 200×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C IF ResetMouse anti Rat CD43
- Product Type
- Monoclonal Antibody
- Clone
- W3/13
- Isotype
- IgG1
- Specificity
- CD43
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001) Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 Pack Size: 1 mg, 0.1 mg 1/25 Pack Size: 0.25 mg |
1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD43 antibody
-
Brown, W.R.A. et al. (1981) Identification of a glycophorin-like molecule at the cell surface of rat thymocytes.
Nature. 289: 456-460. -
Barclay, A. N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42: 593-600 -
Jung, S. et al. (1994) Therapeutic effect of transforming growth factor-beta 2 on actively induced EAN but not adoptive transfer EAN
Immunology. 83: 545-551. -
Bataller, R. et al. (2003) Prolonged infusion of angiotensin II into normal rats induces stellate cell activation and proinflammatory events in liver.
Am J Physiol Gastrointest Liver Physiol. 285: G642-651 -
Yamanaka, Y. et al. (2011) Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers.
Int Endod J. 44: 669-75. -
Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
J Neuroimmunol. 163: 185-9. -
Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
J Comp Neurol. 487: 166-75. -
Zhang, Z. et al. (2008) FTY720 ameliorates experimental autoimmune neuritis by inhibition of lymphocyte and monocyte infiltration into peripheral nerves.
Exp Neurol. 210: 681-90.
View The Latest Product References
-
Xu, K. et al. (2016) Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 11 (1): 72. -
Dort, J. et al. (2016) Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
Biomed Res Int. 2016: 5214561. -
Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
Neurol Sci. 37 (2): 199-203. -
Rice, E.K. et al. (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II.
Kidney Int. 63 (4): 1265-75. -
Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
Muscle Nerve. 48 (3): 403-14. -
Dort, J. et al. (2012) Beneficial effects of cod protein on skeletal muscle repair following injury.
Appl Physiol Nutr Metab. 37 (3): 489-98. -
Ornellas, F.M. et al. (2019) Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model.
Sci Rep. 9 (1): 19604. -
Grad, E. et al. (2019) Monocyte Modulation by Liposomal Alendronate Improves Cardiac Healing in a Rat Model of Myocardial Infarction
Regenerative Engineering and Translational Medicine. 5 (3): 280-9. -
Kami, K. et al. (2019) Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat.
J Histochem Cytochem. 67 (11): 791-9. -
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
Biomedicines. 10 (5): 1021.
Further Reading
-
Forbes, R. D. C. et al. (1983) Leukocyte subsets in first-set rat cardiac allograft rejection. A serial immunohistologic study using monoclonal antibodies
Transplantation. 36: 681-686
- Synonyms
- Leukosialin
- RRID
- AB_2286557
- UniProt
- P13838
- Entrez Gene
- Spn
- GO Terms
- GO:0002544 chronic inflammatory response
- GO:0016021 integral to membrane
- GO:0005902 microvillus
- GO:0009986 cell surface
- GO:0019904 protein domain specific binding
- GO:0032154 cleavage furrow
- GO:0042117 monocyte activation
- GO:0071594 thymocyte aggregation
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