CD200 antibody | OX-2

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Mouse anti Rat CD200

Product Type
Monoclonal Antibody
Clone
OX-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA44GA C EM F IF IP 0.1 mg
MCA44R C EM F IF IP 0.25 mg
MCA44G C EM F IF IP 1 mg
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.

Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).

This product is routinely tested by flow cytometry on rat thymocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04218 Related reagents
Entrez Gene
Cd200 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0042802 identical protein binding
Regulatory
For research purposes only

Applications of CD200 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Application Based External Images

Immuno-electron Microscopy

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD200 antibody

  1. Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
    Immunology. 44 (4): 727-36.
  2. Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
    J Neurochem. 43 (4): 1061-7.
  3. Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
    Neurosci Lett. 418: 44-8.
  4. Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
    J Immunol. 158 (10): 4548-54.
  5. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42: 170-6.
  6. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.
  7. Preston, S. et al. (1997) The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages.
    Eur J Immunol. 27: 1911-8.
  8. Bartolome, M.V. et al. (2002) Transitional expression of OX-2 and GAP-43 glycoproteins in developing rat cochlear nerve fibers.
    Histol Histopathol. 17: 83-95.
  9. Tsai, M.H. et al. (2015) CD200 in growing rat lungs: developmental expression and control by dexamethasone.
    Cell Tissue Res. 359 (3): 729-42.

Further Reading

  1. Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
    EMBO J. 4 (1): 113-8.