CD200 antibody | OX-2

Product Code Applications Pack Size List Price Your Price Qty
MCA44G
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SDS Safety Datasheet SDS
C EM F IF IP 1 mg loader
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loader
MCA44GA
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SDS Safety Datasheet SDS
C EM F IF IP 0.1 mg loader
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loader
MCA44R
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SDS Safety Datasheet SDS
C EM F IF IP 0.25 mg loader
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Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.

The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation (Ko et al. 2009).

Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
MCA44G, MCA44R: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA44GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
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Description Mouse IgG1 Negative Control

References for CD200 antibody

  1. Mcmaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
    Eur J Immunol. 9 (6): 426-33.
  2. Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52 (4): 631-40.
  3. Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
    Immunology. 44 (4): 727-36.
  4. Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
    J Neurochem. 43 (4): 1061-7.
  5. Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
    Neurosci Lett. 418: 44-8.
  6. Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
    J Immunol. 158 (10): 4548-54.
  7. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42: 170-6.
  8. Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.
  9. View The Latest Product References
  10. Preston, S. et al. (1997) The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages.
    Eur J Immunol. 27: 1911-8.
  11. Bartolome, M.V. et al. (2002) Transitional expression of OX-2 and GAP-43 glycoproteins in developing rat cochlear nerve fibers.
    Histol Histopathol. 17: 83-95.
  12. Tsai, M.H. et al. (2015) CD200 in growing rat lungs: developmental expression and control by dexamethasone.
    Cell Tissue Res. 359 (3): 729-42.

Further Reading

  1. Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
    EMBO J. 4 (1): 113-8.

Immuno-electron Microscopy

Immunofluorescence

Immunohistology - Frozen

Synonyms
OX2
RRID
AB_322354
UniProt
P04218
Entrez Gene
Cd200
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0042802 identical protein binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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