CD200 antibody | OX-2
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.
Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).
This product is routinely tested by flow cytometry on rat thymocytes.
Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).
This product is routinely tested by flow cytometry on rat thymocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P04218 Related reagents
- Entrez Gene
- Cd200 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0042802 identical protein binding
- Regulatory
- For research purposes only
Applications of CD200 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immuno-electron Microscopy | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F |
Application Based External Images
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Product Specific References
References for CD200 antibody
-
Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
Immunology. 44 (4): 727-36. -
Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
J Neurochem. 43 (4): 1061-7. -
Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
Neurosci Lett. 418: 44-8. -
Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
J Immunol. 158 (10): 4548-54. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6. -
Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95. -
Preston, S. et al. (1997) The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages.
Eur J Immunol. 27: 1911-8. -
Bartolome, M.V. et al. (2002) Transitional expression of OX-2 and GAP-43 glycoproteins in developing rat cochlear nerve fibers.
Histol Histopathol. 17: 83-95. -
Tsai, M.H. et al. (2015) CD200 in growing rat lungs: developmental expression and control by dexamethasone.
Cell Tissue Res. 359 (3): 729-42.
Further Reading
-
Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
EMBO J. 4 (1): 113-8.
