CD200 antibody | OX-2
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.
Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).
This product is routinely tested by flow cytometry on rat thymocytes.
Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).
This product is routinely tested by flow cytometry on rat thymocytes.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04218
- Entrez Gene
- Cd200
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0042802 identical protein binding
- Regulatory
- For research purposes only
Applications of CD200 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 | |
| Immuno-electron Microscopy | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Application Based External Images
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Product Specific References
References for CD200 antibody
-
Mcmaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 9 (6): 426-33. -
Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52 (4): 631-40. -
Barclay, A.N. (1981) Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
Immunology. 44 (4): 727-36. -
Webb, M. & Barclay, A.N. (1984) Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
J Neurochem. 43 (4): 1061-7. -
Matsumoto, H. et al. (2007) Expression of CD200 by macrophage-like cells in ischemic core of rat brain after transient middle cerebral artery occlusion.
Neurosci Lett. 418: 44-8. -
Borriello, F. et al. (1997) MRC OX-2 defines a novel T cell costimulatory pathway.
J Immunol. 158 (10): 4548-54. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6. -
Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95. -
Preston, S. et al. (1997) The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages.
Eur J Immunol. 27: 1911-8. -
Bartolome, M.V. et al. (2002) Transitional expression of OX-2 and GAP-43 glycoproteins in developing rat cochlear nerve fibers.
Histol Histopathol. 17: 83-95. -
Tsai, M.H. et al. (2015) CD200 in growing rat lungs: developmental expression and control by dexamethasone.
Cell Tissue Res. 359 (3): 729-42.
Further Reading
-
Clark, M.J. et al. (1985) MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.
EMBO J. 4 (1): 113-8.
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