CD18 antibody | WT.3

Mouse anti Rat CD18

Product Type
Monoclonal Antibody
Clone
WT.3
Isotype
IgG1
Specificity
CD18

Product Code Applications Pack Size List Price Your Price Qty
MCA775
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Mouse anti Rat CD18 antibody, clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes.

Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
FTL-43.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Lymph node cells from an immunized BALB/c mouse were fused with cells of the PAI mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD18 antibody

  1. Tamatani, T. & Miyasaka, M. (1990) Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells.
    Int Immunol. 2 (2): 165-71.
  2. Tamatani, T. et al. (1991) Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.
    Eur J Immunol. 21 (3): 627-33.
  3. Tamatani, T. et al. (1991) Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in the interaction between lymphocytes and high endothelial cells.
    Eur J Immunol. 21 (3): 855-8.
  4. Alvarez, A. et al. (2007) Gastric antisecretory drugs induce leukocyte-endothelial cell interactions through gastrin release and activation of CCK-2 receptors.
    J Pharmacol Exp Ther. 323. 406-413.
  5. Wu, J.C. et al. (1996) The relationship of adhesion molecules and leukocyte infiltration in chronic tubulointerstitial nephritis induced by puromycin aminonucleoside in Wistar rats.
    Clin Immunol Immunopathol. 79 (3): 229-35.
  6. Nicholls, S.M. et al. (2006) Differences in leukocyte phenotype and interferon-gamma expression in stroma and endothelium during corneal graft rejection.
    Exp Eye Res. 83 (2): 339-47.
  7. Ishida, S. et al. (2003) Leukocytes mediate retinal vascular remodeling during development and vaso-obliteration in disease.
    Nat Med. 9 (6): 781-8.
  8. Fabian, R.H. & Kent, T.A. (1999) Superoxide anion production during reperfusion is reduced by an antineutrophil antibody after prolonged cerebral ischemia.
    Free Radic Biol Med. 26 (3-4): 355-61.
  9. View The Latest Product References
  10. Shen K et al. (1995) Circulating leukocyte counts, activation, and degranulation in Dahl hypertensive rats.
    Circ Res. 76 (2): 276-83.
  11. Nutile-McMenemy, N. et al. (2007) Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression.
    J Neurochem. 103 (5): 2035-46.
  12. Martinelli, R. et al. (2009) ICAM-1-mediated endothelial nitric oxide synthase activation via calcium and AMP-activated protein kinase is required for transendothelial lymphocyte migration.
    Mol Biol Cell. 20 (3): 995-1005.
  13. Herrmann, I.K. et al. (2015) Differentiating sepsis from non-infectious systemic inflammation based on microvesicle-bacteria aggregation.
    Nanoscale. 7 (32): 13511-20.
  14. Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
    Nat Commun. 10 (1): 893.
  15. Dragoni, S. et al. (2020) Endothelial Protease Activated Receptor 1 (PAR1) Signalling Is Required for Lymphocyte Transmigration across Brain Microvascular Endothelial Cells.
    Cells. 9(12):2723.

Synonyms
Integrin Beta 2 Chain
RRID
AB_2128469
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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