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CD18 antibody | WT.3

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Anti Rat CD18 Antibody, clone WT.3 thumbnail image 1 Anti Rat CD18 Antibody, clone WT.3 thumbnail image 2
Anti Rat CD18 Antibody, clone WT.3 gallery image 1
Figure A. RPE conjugated mouse anti rat CD4 (MCA55PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat CD4 (MCA55PE) and FITC conjugated mouse anti rat CD18 (MCA775F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD18 Antibody, clone WT.3 gallery image 2
Figure A. FITC conjugated mouse anti rat CD18 (MCA775F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD18 (MCA775F) and RPE conjugated mouse anti rat CD4 (MCA55PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse anti Rat CD18:FITC

Mouse anti Rat CD18

Mouse anti Rat CD18:RPE

Product Type
Monoclonal Antibody
Clone
WT.3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA775F
Datasheet
F
SDS
0.1 mg loader

MCA775
Datasheet
C F IP
SDS
0.2 mg loader

MCA775PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Rat CD18 antibody, clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes.

Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
FTL-43.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Lymph node cells from an immunized BALB/c mouse were fused with cells of the PAI mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader

Product Specific References

References for CD18 antibody

  1. Tamatani, T. & Miyasaka, M. (1990) Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells.
    Int Immunol. 2 (2): 165-71.
  2. Tamatani, T. et al. (1991) Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.
    Eur J Immunol. 21 (3): 627-33.
  3. Tamatani, T. et al. (1991) Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in the interaction between lymphocytes and high endothelial cells.
    Eur J Immunol. 21 (3): 855-8.
  4. Alvarez, A. et al. (2007) Gastric antisecretory drugs induce leukocyte-endothelial cell interactions through gastrin release and activation of CCK-2 receptors.
    J Pharmacol Exp Ther. 323. 406-413.
  5. Wu JC et al. (1996) The relationship of adhesion molecules and leukocyte infiltration in chronic tubulointerstitial nephritis induced by puromycin aminonucleoside in Wistar rats.
    Clin Immunol Immunopathol. 79 (3): 229-35.
  6. Nicholls, S.M. et al. (2006) Differences in leukocyte phenotype and interferon-gamma expression in stroma and endothelium during corneal graft rejection.
    Exp Eye Res. 83 (2): 339-47.
  7. Ishida, S. et al. (2003) Leukocytes mediate retinal vascular remodeling during development and vaso-obliteration in disease.
    Nat Med. 9 (6): 781-8.
  8. Fabian, R.H. & Kent, T.A. (1999) Superoxide anion production during reperfusion is reduced by an antineutrophil antibody after prolonged cerebral ischemia.
    Free Radic Biol Med. 26 (3-4): 355-61.
  9. Shen K et al. (1995) Circulating leukocyte counts, activation, and degranulation in Dahl hypertensive rats.
    Circ Res. 76 (2): 276-83.
  10. Nutile-McMenemy, N. et al. (2007) Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression.
    J Neurochem. 103 (5): 2035-46.
  11. Martinelli, R. et al. (2009) ICAM-1-mediated endothelial nitric oxide synthase activation via calcium and AMP-activated protein kinase is required for transendothelial lymphocyte migration.
    Mol Biol Cell. 20 (3): 995-1005.
  12. Herrmann, I.K. et al. (2015) Differentiating sepsis from non-infectious systemic inflammation based on microvesicle-bacteria aggregation.
    Nanoscale. 7 (32): 13511-20.
  13. Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
    Nat Commun. 10 (1): 893.

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