CD161 antibody | 10/78
Phycoerythrin conjugated Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427PE) used for the identification of NK cells in rat blood samples by flow cytometry.
Image caption:
Graft survival in NK cell depleted recipients. A: 3-week-old (young) Lewis rats were injected twice with a NK cell depleting antibody (3.2.3). Blood was taken and analyzed by flow cytometry for the presence of CD3+ and CD161+ cells. Intraperitoneal injection of 3.2.3 lead to complete absence of CD161+ NK/NKT cells and did not affect cell numbers of CD3+CD161- T cells. FACS data shown are representative for control animals that received an isotypic antibody (NK+) or 3.2.3-treated rats (NK-). B: After injections of either isotyp controls (NK+) or 3.2.3 (NK-) allogeneic penetrating keratoplasty was performed and the grafts were assessed clinically until rejection occurred. Albeit all recipients of both allogeneic groups rejected, animals of group NK- showed a statistically significantly delay of immunological allograft failure (p<0.01). None of the syngeinic controls (groups syn.NK+ and syn.NK-, respectively) rejected. C: NK cells were depleted in 10-week-old (old) Lewis rats (NK-) or isotypic antibody was injected (NK+). NK cell depletion had no effect on graft survival rates.
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427) used to demonstrate CD161 expressing cells in rat corneal tissue by immunohistochemistry on cryostat sections.
Image caption:
Analysis of innate immune cells into a corneal graft. CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown CD161 (E) in NK deficient and control animals. F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427) used to evaluate frequency of CD161 expressing cells in rat pup hippocampal tissue by immunofluorescence.
Image caption:
TAK-242 treatment affects the distribution of neutrophils, natural killer (NK) cells, and T cells after HIBD in neonates. (B) The positive expression of CD161 represents the distribution of NK cells in the hippocampal CA1 region at 24 h and 7 days after HI. Scale bar: 20 μm.(E) The difference in the number of CD161+ cells in the CA1 region among groups. n=4. *P<0.05, **P<0.01, and ***P<0.001 vs the control group; & P<0.05 and && P<0.01 vs the HI group.
From: Xing Z, Zhen T, Jie F, Jie Y, Shiqi L, Kaiyi Z, Zhicui O, Mingyan H.
Early Toll-like receptor 4 inhibition improves immune dysfunction in the hippocampus after hypoxic-ischemic brain damage.
Int J Med Sci 2022; 19(1):142-151.
doi:10.7150/ijms.66494.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- 10/78
- Isotype
- IgG1
- Specificity
- CD161
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78 recognizes both forms of CD161 (Li et al. 2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen. Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified splenic NK cells from the LEW rat strain.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Radioimmunoassays | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD161 antibody
-
Sedgwick, J.D. et al. (1998) Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts.
J Immunol. 160 (11): 5320-30. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58 (1): 100-14. -
Tliba, O. et al. (2002) Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats.
Vet Res. 33 (3): 327-32. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Dyugovskaya, L. et al. (2003) Phenotypic profile and functional characterization of rat lymph node-derived gammadelta T cells: implication in the immune response to cytomegalovirus.
Immunology. 108 (2): 129-36. -
Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
Cancer Res. 65: 7194-204. -
Obara, H. et al. (2005) IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.
Am J Transplant. 5 (9): 2094-103. -
Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121: 555-64.
View The Latest Product References
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Blöcher, S. et al. (2007) Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.
Clin Immunol. 124 (1): 98-108. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Wrann, C.D. et al. (2010) Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
Exp Toxicol Pathol. 62 (1): 1-8. -
Lyons, A. et al. (2011) Atorvastatin prevents age-related and amyloid-beta-induced microglial activation by blocking interferon-gamma release from natural killer cells in the brain.
J Neuroinflammation. 8: 27. -
Kuper, C.F. et al. (2011) Oxazolone (OXA) is a respiratory allergen in Brown Norway rats.
Toxicology. 290 (1): 59-68. -
Lee, J.S. et al. (2011) Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents.
Food Chem. 126 (4): 1521-6. -
Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
Cell Mol Immunol. 9 (2): 163-74. -
Beutel, G. et al. (2013) Effect of chronic elevated asymmetric dimethylarginine (ADMA) levels on granulopoiesis.
Ann Hematol. 92 (4): 505-8. -
Arsenović-Ranin, N. et al. (2013) Ovarian hormone withdrawal in prepubertal developmental stage does not prevent thymic involution in rats.
Exp Biol Med (Maywood). 238 (6): 641-57. -
Arndt, T. et al. (2014) Variable immune cell frequencies in peripheral blood of LEW.1AR1-iddm rats over time compared to other congenic LEW strains.
Clin Exp Immunol. 177 (1): 168-78. -
Djikić J et al. (2014) Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.
Exp Gerontol. 58: 179-97. -
Koch, M. et al. (2015) Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.
Stem Cells Int. 2015: 486141. -
Lemke, A. et al. (2015) Rat renal transplant model for mixed acute humoral and cellular rejection: Weak correlation of serum cytokines/chemokines with intragraft changes.
Transpl Immunol. 33 (2): 95-102. -
Williamson, L.L. et al. (2016) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.
Brain Behav Immun. 51: 14-28. -
Bähr, I. et al. (2017) Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence.
J Nutr Metab. 2017: 4297025. -
Sun, C.K. et al. (2017) Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury.
Am J Transl Res. 9 (4): 1543-60. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Xing, Z. et al. (2021) Early Toll-like receptor 4 inhibition improves immune dysfunction in the hippocampus after hypoxic-ischemic brain damage
Int J Med Sci. 19 (1): 142-51.
- Synonyms
- NKR-P1A
- RRID
- AB_2234351
- UniProt
- P27471
- A4KWA1
- Entrez Gene
- Klrb1b
- Klrb1a
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005529 sugar binding
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