CD161 antibody | 10/78

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MCA1427GA
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C F IP 0.1 mg loader
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MCA1427
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C F IP R WB 0.25 mg loader
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Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78  recognizes both forms of CD161 (Li et al. 2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen.

Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified splenic NK cells from the LEW rat strain.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Radioimmunoassays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Sugggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD161 antibody

  1. Li, J. et al. (2003) Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B.
    Int Immunol. 15: 411-6.
  2. Dyugovskaya, L. et al. (2003) Phenotypic profile and functional characterization of rat lymph node-derived gammadelta T cells: implication in the immune response to cytomegalovirus.
    Immunology. 108 (2): 129-36.
  3. Sedgwick, J.D. et al. (1998) Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts.
    J Immunol. 160 (11): 5320-30.
  4. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  5. Lyons, A. et al. (2011) Atorvastatin prevents age-related and amyloid-beta-induced microglial activation by blocking interferon-gamma release from natural killer cells in the brain.
    J Neuroinflammation. 8: 27.
  6. Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
    Cancer Res. 65: 7194-204.
  7. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87: 1515-22.
  8. Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
    Immunology. 121: 555-64.
  9. View The Latest Product References
  10. Tliba, O. et al. (2002) Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats.
    Vet Res. 33 (3): 327-32.
  11. Blöcher, S. et al. (2007) Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.
    Clin Immunol. 124 (1): 98-108.
  12. Koch, M. et al. (2015) Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.
    Stem Cells Int. 2015: 486141.
  13. Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
    Cell Mol Immunol. 9 (2): 163-74.
  14. Wrann, C.D. et al. (2010) Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
    Exp Toxicol Pathol. 62 (1): 1-8.
  15. Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
    Kidney Int. 58 (1): 100-14.
  16. Obara, H. et al. (2005) IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.
    Am J Transplant. 5 (9): 2094-103.
  17. Beutel, G. et al. (2013) Effect of chronic elevated asymmetric dimethylarginine (ADMA) levels on granulopoiesis.
    Ann Hematol. 92 (4): 505-8.
  18. Lee, J.S. et al. (2011) Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents.
    Food Chem. 126 (4): 1521-6.
  19. Williamson, L.L. et al. (2016) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.
    Brain Behav Immun. 51: 14-28.
  20. Arndt, T. et al. (2014) Variable immune cell frequencies in peripheral blood of LEW.1AR1-iddm rats over time compared to other congenic LEW strains.
    Clin Exp Immunol. 177 (1): 168-78.
  21. Kuper, C.F. et al. (2011) Oxazolone (OXA) is a respiratory allergen in Brown Norway rats.
    Toxicology. 290 (1): 59-68.
  22. Arsenović-Ranin, N. et al. (2013) Ovarian hormone withdrawal in prepubertal developmental stage does not prevent thymic involution in rats.
    Exp Biol Med (Maywood). 238 (6): 641-57.
  23. Djikić J et al. (2014) Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.
    Exp Gerontol. 58: 179-97.
  24. Lemke, A. et al. (2015) Rat renal transplant model for mixed acute humoral and cellular rejection: Weak correlation of serum cytokines/chemokines with intragraft changes.
    Transpl Immunol. 33 (2): 95-102.
  25. Bähr, I. et al. (2017) Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence.
    J Nutr Metab. 2017: 4297025.
  26. Sun, C.K. et al. (2017) Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury.
    Am J Transl Res. 9 (4): 1543-60.
  27. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  28. Xing, Z. et al. (2021) Early Toll-like receptor 4 inhibition improves immune dysfunction in the hippocampus after hypoxic-ischemic brain damage
    Int J Med Sci. 19 (1): 142-51.

Flow Cytometry

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
NKR-P1A
RRID
AB_2234351
UniProt
P27471
A4KWA1
Entrez Gene
Klrb1b
Klrb1a
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
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