CD161 antibody | 10/78
Phycoerythrin conjugated Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427PE) used for the identification of NK cells in rat blood samples by flow cytometry.
Image caption:
Graft survival in NK cell depleted recipients. A: 3-week-old (young) Lewis rats were injected twice with a NK cell depleting antibody (3.2.3). Blood was taken and analyzed by flow cytometry for the presence of CD3+ and CD161+ cells. Intraperitoneal injection of 3.2.3 lead to complete absence of CD161+ NK/NKT cells and did not affect cell numbers of CD3+CD161- T cells. FACS data shown are representative for control animals that received an isotypic antibody (NK+) or 3.2.3-treated rats (NK-). B: After injections of either isotyp controls (NK+) or 3.2.3 (NK-) allogeneic penetrating keratoplasty was performed and the grafts were assessed clinically until rejection occurred. Albeit all recipients of both allogeneic groups rejected, animals of group NK- showed a statistically significantly delay of immunological allograft failure (p<0.01). None of the syngeinic controls (groups syn.NK+ and syn.NK-, respectively) rejected. C: NK cells were depleted in 10-week-old (old) Lewis rats (NK-) or isotypic antibody was injected (NK+). NK cell depletion had no effect on graft survival rates.
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.
Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427) used to demonstrate CD161 expressing cells in rat corneal tissue by immunohistochemistry on cryostat sections.
Image caption:
Analysis of innate immune cells into a corneal graft. CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown CD161 (E) in NK deficient and control animals. F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.
Mouse anti Rat CD161:Alexa Fluor® 488
Mouse anti Rat CD161:Alexa Fluor® 647
Mouse anti Rat CD161:Preservative Free
Mouse anti Rat CD161:FITC
Mouse anti Rat CD161
Mouse anti Rat CD161:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 10/78
- Isotype
- IgG1
Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78 recognizes both forms of CD161 (Li et al. 2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen.
Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010).
Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Purified splenic NK cells from the LEW rat strain.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P27471 Related reagents
- A4KWA1 Related reagents
- Entrez Gene
- Klrb1b Related reagents
- Klrb1a Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005529 sugar binding
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD161 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Sugggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Sugggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA1209A488 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Preservative Free | MCA1209XZ | 1 mg | F | ||
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:RPE | MCA1209PE | 100 Tests | F |
Application Based External Images
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for CD161 antibody
-
Li, J. et al. (2003) Expression cloning and function of the rat NK activating and inhibitory receptors NKR-P1A and -P1B.
Int Immunol. 15: 411-6. -
Sedgwick, J.D. et al. (1998) Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts.
J Immunol. 160 (11): 5320-30. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Lyons, A. et al. (2011) Atorvastatin prevents age-related and amyloid-beta-induced microglial activation by blocking interferon-gamma release from natural killer cells in the brain.
J Neuroinflammation. 8: 27. -
Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
Cancer Res. 65: 7194-204. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121: 555-64. -
Tliba, O. et al. (2002) Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats.
Vet Res. 33 (3): 327-32. -
Blöcher, S. et al. (2007) Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.
Clin Immunol. 124 (1): 98-108. -
Koch, M. et al. (2015) Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.
Stem Cells Int. 2015: 486141. -
Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
Cell Mol Immunol. 9 (2): 163-74. -
Wrann, C.D. et al. (2010) Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
Exp Toxicol Pathol. 62 (1): 1-8. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58 (1): 100-14. -
Obara, H. et al. (2005) IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.
Am J Transplant. 5 (9): 2094-103. -
Beutel, G. et al. (2013) Effect of chronic elevated asymmetric dimethylarginine (ADMA) levels on granulopoiesis.
Ann Hematol. 92 (4): 505-8. -
Lee, J.S. et al. (2011) Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents.
Food Chem. 126 (4): 1521-6. -
Williamson, L.L. et al. (2016) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.
Brain Behav Immun. 51: 14-28. -
Arndt, T. et al. (2014) Variable immune cell frequencies in peripheral blood of LEW.1AR1-iddm rats over time compared to other congenic LEW strains.
Clin Exp Immunol. 177 (1): 168-78. -
Kuper, C.F. et al. (2011) Oxazolone (OXA) is a respiratory allergen in Brown Norway rats.
Toxicology. 290 (1): 59-68. -
Arsenović-Ranin, N. et al. (2013) Ovarian hormone withdrawal in prepubertal developmental stage does not prevent thymic involution in rats.
Exp Biol Med (Maywood). 238 (6): 641-57. -
Djikić J et al. (2014) Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.
Exp Gerontol. 58: 179-97. -
Lemke, A. et al. (2015) Rat renal transplant model for mixed acute humoral and cellular rejection: Weak correlation of serum cytokines/chemokines with intragraft changes.
Transpl Immunol. 33 (2): 95-102. -
Bähr Ina et al. (2017) Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence
Journal of Nutrition and Metabolism. 2017: 1-14. -
Sun, C.K. et al. (2017) Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury.
Am J Transl Res. 9 (4): 1543-60.
