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CD8 antibody | 12.C7

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Anti Rabbit CD8 Antibody, clone 12.C7 thumbnail image 1 Anti Rabbit CD8 Antibody, clone 12.C7 thumbnail image 2
Anti Rabbit CD8 Antibody, clone 12.C7 gallery image 1
Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA) followed by FITC conjugated Goat anti Mouse IgG (H/L) antibody (STAR117F)
Anti Rabbit CD8 Antibody, clone 12.C7 gallery image 2
Published customer image:
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.
Image caption:
Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.
In vivo
BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry. A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis). B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P<0.05; **P<0.005, *** P<0.0001). In the left column the following symbols were used: small closed triangle, MR17/1 and IR17/1; small open square, MR17/2 and IR17/2; small closed circle, MR20 and IR20; large open circle, MR24 and IR24.

From: Dewals B, Boudry C, Farnir F, Drion P-V, Vanderplasschen A (2008)
Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8+ T Cells Supporting a Latent Infection.
PLoS ONE 3(2): e1627.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Rabbit CD8:FITC

Mouse anti Rabbit CD8

Product Type
Monoclonal Antibody
Clone
12.C7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1576F
Datasheet
F
MSDS
0.1 mg loader

MCA1576GA
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.

Product Details

Target Species
Rabbit
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/100 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader

Product Specific References

References for CD8 antibody

  1. De Smet, W. et al. (1983) Rabbit leukocyte surface antigens defined by monoclonal antibodies.
    Eur J Immunol. 13: 919-28.
  2. Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
    PLos ONE 3: e1627.
  3. Zhao, L. et al. (2011) Evaluation of immunocompatibility of tissue-engineered periosteum.
    Biomed Mater.6:015005.
  4. Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
    Immunology. 77: 106-8.
  5. Marques, R.M. et al. (2012) Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection.
    Vet Immunol Immunopathol. 150: 181-8.
  6. Schock, A. and Reid, H.W. (1996) Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever.
    Vet Microbiol. 53: 111-9.
  7. Beghelli, D. et al. (2012) Phytoderivates in Rabbit Diet and Immune responses.
    Proceedings 10th World Rabbit Congress: 1019-23
  8. Khan AA et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
    J Virol. 89 (13): 6619-32.
  9. Srivastava, R. et al. (2015) A Herpes Simplex Virus Type 1 Human Asymptomatic CD8+ T-Cell Epitopes-Based Vaccine Protects Against Ocular Herpes in a "Humanized" HLA Transgenic Rabbit Model.
    Invest Ophthalmol Vis Sci. 56 (6): 4013-28.
  10. Hanson, N.B. & Lanning, D.K. (2008) Microbial induction of B and T cell areas in rabbit appendix.
    Dev Comp Immunol. 32 (8): 980-91.
  11. Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
    J Leukoc Biol. 85 (1): 44-54.
  12. Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
    J Virol. 85 (14): 6941-54.
  13. Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
    J Comp Pathol. 138 (2-3): 131-44.
  14. Stich N et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
    Toxins (Basel). 2 (9): 2272-88.
  15. Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    J Virol. 89 (7): 3630-47.
  16. Srivastava, R. et al. (2016) The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
    J Virol. 90 (8): 3913-28.
  17. Gates, K.V. & Griffiths, L.G. (2018) Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.
    Immunol Res. 66 (2): 288-98.
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