CD8 antibody | 12.C7
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.
Image caption:
Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody.
In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry. A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis). B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P<0.05; **P<0.005, *** P<0.0001). In the left column the following symbols were used: small closed triangle, MR17/1 and IR17/1; small open square, MR17/2 and IR17/2; small closed circle, MR20 and IR20; large open circle, MR24 and IR24.
From: Dewals B, Boudry C, Farnir F, Drion P-V, Vanderplasschen A (2008)
Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8+ T Cells Supporting a Latent Infection.
PLoS ONE 3(2): e1627.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA used to identify rabbit CD8 positive T cells by immunofluorescence
Image caption:
Gating strategy for CD8+ T cells (PBMCs or CD8TpM) after co-culture with BT or EBL cells.
From: Myster F, Gong M-J, Javaux J, Suárez NM, Wilkie GS, Connelley T, et al. (2020)
Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever.
PLoS Pathog 16(3): e1008405.
doi: 10.1371/journal.ppat.1008405
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576F) used to evaluate CD8 expression on cells HSV-1 infected rabbits by flow cytometry.
Image caption:
Differential gene expression in HSV-specific CD8+ T cells from HSV-1 infected symptomatic vs. asymptomatic individuals.
Frequencies of CD8+ T cells specific to HLA-A*0201-restricted HSV-1 gB561–567 and VP11/12702–710 epitopes detected by FACS in SYMP vs. ASYMP individuals.
From: Prakash S, Roy S, Srivastava R, Coulon PG, Dhanushkodi NR, Vahed H, Jankeel A, Geertsema R, Amezquita C, Nguyen L, Messaoudi I, Burkhardt AM, BenMohamed L.
Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes.
Sci Rep. 2020 Aug 14;10(1):13843.
doi: 10.1038/s41598-020-70673-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Rabbit CD8
- Product Type
- Monoclonal Antibody
- Clone
- 12.C7
- Isotype
- IgG1
- Specificity
- CD8
Antibody Quality and 
Unrivaled 
| Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity. |
- Target Species
- Rabbit
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD8 antibody
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Civin, C.I. et al. (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.
J Immunol. 133 (1): 157-65. -
De Smet, W. et al. (1983) Rabbit leukocyte surface antigens defined by monoclonal antibodies.
Eur J Immunol. 13: 919-28. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627. -
Zhao, L. et al. (2011) Evaluation of immunocompatibility of tissue-engineered periosteum.
Biomed Mater.6:015005. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77: 106-8. -
Marques, R.M. et al. (2012) Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection.
Vet Immunol Immunopathol. 150: 181-8. -
Schock, A. and Reid, H.W. (1996) Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever.
Vet Microbiol. 53: 111-9. -
Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
J Comp Pathol. 138 (2-3): 131-44.
View The Latest Product References
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Khan AA et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Srivastava, R. et al. (2015) A Herpes Simplex Virus Type 1 Human Asymptomatic CD8+ T-Cell Epitopes-Based Vaccine Protects Against Ocular Herpes in a "Humanized" HLA Transgenic Rabbit Model.
Invest Ophthalmol Vis Sci. 56 (6): 4013-28. -
Hanson, N.B. & Lanning, D.K. (2008) Microbial induction of B and T cell areas in rabbit appendix.
Dev Comp Immunol. 32 (8): 980-91. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
J Virol. 85 (14): 6941-54. -
Khan, A.A. et al. (2018) Human Asymptomatic Epitope Peptide/CXCL10-Based Prime/Pull Vaccine Induces Herpes Simplex Virus-Specific Gamma Interferon-Positive CD107+ CD8+ T Cells That Infiltrate the Corneas and Trigeminal Ganglia of Humanized HLA Transgenic Rabbits and Protect against Ocular Herpes Challenge.
J Virol. 92 (16): e00535-18. -
Stich N et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
Toxins (Basel). 2 (9): 2272-88. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Srivastava, R. et al. (2016) The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
J Virol. 90 (8): 3913-28. -
Gates, K.V. & Griffiths, L.G. (2018) Chronic graft-specific cell-mediated immune response toward candidate xenogeneic biomaterial.
Immunol Res. 66 (2): 288-98. -
Prakash, S. et al. (2020) Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes.
Sci Rep. 10 (1): 13843. -
Jeklova, E. et al. (2020) Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi..
Vet Res. 51 (1): 79. -
Myster, F. et al. (2020) Alcelaphine herpesvirus 1 genes A7 and A8 regulate viral spread and are essential for malignant catarrhal fever.
PLoS Pathog. 16 (3): e1008405. -
Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
Pol J Vet Sci. 23 (1): 37-42. -
Niedźwiedzka-Rystwej, P. et al. (2021) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
Vet Res Commun. Oct 29 [Epub ahead of print].
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