CD8 antibody | 12.C7
Mouse anti Rabbit CD8 antibody, clone 12.C7 (MCA1576GA) used for the evaluation of CD8 expression on cells from lymph nodes and spleen from rabbits infected with alcelaphine herpesvirus 1 by flow cytometry.
Image caption:
Analysis of in vivo BrdU incorporation. Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody. In vivo BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry. A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis). B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P<0.05; **P<0.005, *** P<0.0001). In the left column the following symbols were used: small closed triangle, MR17/1 and IR17/1; small open square, MR17/2 and IR17/2; small closed circle, MR20 and IR20; large open circle, MR24 and IR24.
From: Dewals B, Boudry C, Farnir F, Drion P-V, Vanderplasschen A (2008) Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8+ T Cells Supporting a Latent Infection. PLoS ONE 3(2): e1627.
Mouse anti Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/100 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F |
Product Specific References
References for CD8 antibody
-
De Smet, W. et al. (1983) Rabbit leukocyte surface antigens defined by monoclonal antibodies.
Eur J Immunol. 13: 919-28. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627. -
Zhao, L. et al. (2011) Evaluation of immunocompatibility of tissue-engineered periosteum.
Biomed Mater.6:015005. -
Wilkinson, J.M. et al. (1992) A cytotoxic rabbit T-cell line infected with a gamma-herpes virus which expresses CD8 and class II antigens.
Immunology. 77: 106-8. -
Marques, R.M. et al. (2012) Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection.
Vet Immunol Immunopathol. 150: 181-8. -
Schock, A. and Reid, H.W. (1996) Characterisation of the lymphoproliferation in rabbits experimentally affected with malignant catarrhal fever.
Vet Microbiol. 53: 111-9. -
Beghelli, D. et al. (2012) Phytoderivates in Rabbit Diet and Immune responses.
Proceedings 10th World Rabbit Congress: 1019-23 -
Khan AA et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Srivastava, R. et al. (2015) A Herpes Simplex Virus Type 1 Human Asymptomatic CD8+ T-Cell Epitopes-Based Vaccine Protects Against Ocular Herpes in a "Humanized" HLA Transgenic Rabbit Model.
Invest Ophthalmol Vis Sci. 56 (6): 4013-28. -
Hanson, N.B. & Lanning, D.K. (2008) Microbial induction of B and T cell areas in rabbit appendix.
Dev Comp Immunol. 32 (8): 980-91. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
J Virol. 85 (14): 6941-54. -
Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
J Comp Pathol. 138 (2-3): 131-44. -
Stich N et al. (2010) Staphylococcal superantigen (TSST-1) mutant analysis reveals that t cell activation is required for biological effects in the rabbit including the cytokine storm.
Toxins (Basel). 2 (9): 2272-88. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Srivastava, R. et al. (2016) The Herpes Simplex Virus Latency-Associated Transcript Gene Is Associated with a Broader Repertoire of Virus-Specific Exhausted CD8+ T Cells Retained within the Trigeminal Ganglia of Latently Infected HLA Transgenic Rabbits.
J Virol. 90 (8): 3913-28.
