CD25 antibody | KEI-alpha1
Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 (MCA1119GA) used to label CD25+ activated lymphocytes for analysis by flow cytometry.
Image caption:
CD25 and CD44 expression in CD8+ T cells isolated from lymphoid and non-lymphoid organs during WD-MCF in rabbits. At time of euthanasia, analysis by multi-colour flow cytometry was conducted on PBMC and mononuclear leukocytes isolated from pLN, spleen, liver, lung and kidney of mock-infected or AlHV-1-infected rabbits developing WD-MCF. (A) Half-offset histograms of CD25 or CD44 expression by CD4+ (left panel) or CD8+ cells (right panel) in mononuclear leukocytes isolated from pLN of one representative rabbit of each group. Data were obtained by triple-staining of CD4 or CD8 with isotype control (grey histograms) or CD25 and CD44 (blue histograms) as described in Methods. (B) Mean fluorescence intensities of CD25 in CD4+ (left panel) or CD8+ cells (right panel) obtained by multi-colour flow cytometry analysis. The percentages of CD4+ or CD8+ cells in the kidney of mock-infected animals were below 3% and were therefore not plotted. Data are representative of two independent experiments (n = 3). Bars show means ± SEM. *p <0.05, **p <0.01.
From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4-
T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42(1):95.
doi: 10.1186/1297-9716-42-95.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit CD25
- Product Type
- Monoclonal Antibody
- Clone
- KEI-alpha1
- Isotype
- IgG2b
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 recognizes rabbit CD25, a 7#126;55 kDa cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes. Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993). |
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Cell membrane preparation of HTLV-1 transformed rabbit T cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunized BALB/c mice were fused with cells of the mouse PAI myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
References for CD25 antibody
-
Kotani, M. et al. (1993) Generation of monoclonal antibodies to the rabbit interleukin-2 receptor alpha chain (CD25) and its distribution in HTLV-1-transformed rabbit T cells.
Jpn J Cancer Res. 84 (7): 770-5. -
Vinuesa, A.M. et al. (2010) Inoculation with Mycobacterium plhei inhibits allergic inflammation in a rabbit model of ovalbumin (ova) sensitization
Rev CES Med Vet Zootec. 5: 10-16 -
D'Agostino, B. et al. (2007) Activation of protease-activated receptor-2 reduces airways inflammation in experimental allergic asthma.
Clin Exp Allergy. 37: 1436-43. -
Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42: 95. -
Guerrero, I. et al. (2011) Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms
Vet Immunol Immunopathol. 140: 75-81. -
Khan, A.A. et al. (2015) Therapeutic immunization with a mixture of herpes simplex virus 1 glycoprotein D-derived “asymptomatic” human CD8+ T-cell epitopes decreases spontaneous ocular shedding in latently infected HLA transgenic rabbits: association with low frequency of local PD-1+ TIM-3+ CD8+ exhausted T cells.
J Virol. 89 (13): 6619-32. -
Penadés, M. et al. (2018) Long-term implications of feed energy source in different genetic types of reproductive rabbit females. II. Immunologic status.
Animal. 12 (9): 1877-85. -
Niedźwiedzka-Rystwej, P. et al. (2020) B and T lymphocytes in rabbits change according to the sex and throughout the year.
Pol J Vet Sci. 23 (1): 37-42.
View The Latest Product References
-
Muñoz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
Am J Pathol. 190 (6): 1188-210. -
Niedźwiedzka-Rystwej, P. et al. (2021) Reactivity of selected markers of innate and adaptive immunity in rabbits experimentally infected with antigenic variants of RHD (Lagovirus europaeus/GI.1a).
Vet Res Commun. Oct 29 [Epub ahead of print]. -
Parameswaran, N. et al. (2014) The A2 gene of alcelaphine herpesvirus-1 is a transcriptional regulator affecting cytotoxicity in virus-infected T cells but is not required for malignant catarrhal fever induction in rabbits.
Virus Res. 188: 68-80.
- Synonyms
- IL-2R Alpha Chain
MCA1119GA
152226
159436
163953
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