CD11b antibody | 198

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Mouse anti Rabbit CD11b

Product Type
Monoclonal Antibody
Clone
198
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA802GA C F IF IP 0.1 mg
Mouse anti Rabbit CD11b antibody, clone 198 recognizes the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1. Mouse anti Rabbit CD11b antibody, clone 198 immunoprecipitates two proteins of molecular weight 165 kD and 95 kD from granulocytes. It recognizes monocytes, macrophages and neutrophils by flow cytometry and is thought to be against the homologue of human CD11b. In immunohistochemistry good staining of macrophages is observed.

Product Details

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit adherent blood leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0.Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD11b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood .

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD11b antibody

  1. Smet, E.G. et al. (1986) Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit.
    Immunology. 59 (3): 419-25.
  2. Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
    J Pathol. 170 (3): 315-20.
  3. Hoefer, I.E. et al. (2005) Aspirin, but not clopidogrel, reduces collateral conductance in a rabbit model of femoral artery occlusion.
    J Am Coll Cardiol. 46 (6): 994-1001.
  4. Vinukonda, G. et al. (2010) Neuroprotection in a rabbit model of intraventricular haemorrhage by cyclooxygenase-2, prostanoid receptor-1 or tumour necrosis factor-alpha inhibition.
    Brain. 133 (Pt 8): 2264-80.
  5. Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
    Transplantation. 89: 409-16.
  6. Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
    PLos ONE 3: e1627.
  7. Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    J Appl Physiol. 95: 969-76.
  8. Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
    Stroke. 39: 3378-88.
  9. Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
    Vet Res. 40: 51.
  10. Vinukonda, G. et al. (2016) Hyaluronidase and Hyaluronan Oligosaccharides Promote Neurological Recovery after Intraventricular Hemorrhage.
    J Neurosci. 36 (3): 872-89.