CD11b antibody | 198

Mouse anti Rabbit CD11b

Product Type
Monoclonal Antibody
Clone
198
Isotype
IgG1
Specificity
CD11b

Product Code Applications Pack Size List Price Your Price Qty
MCA802GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rabbit CD11b antibody, clone 198 recognizes the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1. Mouse anti Rabbit CD11b antibody, clone 198 immunoprecipitates two proteins of molecular weight 165 kD and 95 kD from granulocytes. It recognizes monocytes, macrophages and neutrophils by flow cytometry and is thought to be against the homologue of human CD11b. In immunohistochemistry good staining of macrophages is observed.

Target Species
Rabbit
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rabbit adherent blood leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0.Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood .

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References for CD11b antibody

  1. Smet, E.G. et al. (1986) Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit.
    Immunology. 59 (3): 419-25.
  2. Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
    J Pathol. 170 (3): 315-20.
  3. Hoefer, I.E. et al. (2005) Aspirin, but not clopidogrel, reduces collateral conductance in a rabbit model of femoral artery occlusion.
    J Am Coll Cardiol. 46 (6): 994-1001.
  4. Vinukonda, G. et al. (2010) Neuroprotection in a rabbit model of intraventricular haemorrhage by cyclooxygenase-2, prostanoid receptor-1 or tumour necrosis factor-alpha inhibition.
    Brain. 133 (Pt 8): 2264-80.
  5. Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
    Transplantation. 89: 409-16.
  6. Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
    PLos ONE 3: e1627.
  7. Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    J Appl Physiol. 95: 969-76.
  8. Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
    Stroke. 39: 3378-88.
  9. View The Latest Product References
  10. Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
    Vet Res. 40: 51.
  11. Vinukonda, G. et al. (2016) Hyaluronidase and Hyaluronan Oligosaccharides Promote Neurological Recovery after Intraventricular Hemorrhage.
    J Neurosci. 36 (3): 872-89.
  12. Lin, W. et al. (2020) Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology.
    PLoS One. 15 (12): e0244158.

Flow Cytometry

Immunofluorescence

Synonyms
Integrin Alpha M Chain
MAC-1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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