Protein Kinase C Alpha antibody | 133
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Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the α isoform of protein kinase C. PKC-α is a 672 amino acid enzyme containng a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain (UniProt: P17252).
Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the sequence PQFVHPILQSAV at the C terminus of PKCα.
Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the sequence PQFVHPILQSAV at the C terminus of PKCα.
Product Details
- Species Cross-Reactivity
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Target Species Cross Reactivity Mammals - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Protein kinase C alpha.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P17252 Related reagents
- Entrez Gene
- PRKCA Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006112 energy reserve metabolic process
- GO:0007202 activation of phospholipase C activity
- GO:0050796 regulation of insulin secretion
- GO:0008270 zinc ion binding
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0016071 mRNA metabolic process
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0035403 histone kinase activity (H3-T6 specific)
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0070555 response to interleukin-1
- Regulatory
- For research purposes only
Applications of Protein Kinase C Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | Neat | 1/10 | |
| Western Blotting | 1/10 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Protein Kinase C Alpha antibody
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Donovan, A.J. et al. (2012) Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
Mol Pharmacol. 82 (3): 428-37.
