Protein Kinase C Alpha antibody | 133

Mouse anti Protein Kinase C Alpha

Product Type
Monoclonal Antibody
Clone
133
Isotype
IgG2a
Specificity
Protein Kinase C Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA1572
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C WB 2 ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the α isoform of protein kinase C. PKC-α is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain (UniProt: P17252).

Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the sequence PQFVHPILQSAV at the C terminus of PKCα.

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Protein kinase C alpha.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat 1/10
Western Blotting 1/10 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Protein Kinase C Alpha antibody

  1. Donovan, A.J. et al. (2012) Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
    Mol Pharmacol. 82 (3): 428-37.
  2. Chaney, S.Y. et al. (2016) Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation.
    Mol Vis. 22: 1468-89.

RRID
AB_2284228
UniProt
P17252
Entrez Gene
PRKCA
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006112 energy reserve metabolic process
GO:0007202 activation of phospholipase C activity
GO:0050796 regulation of insulin secretion
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016071 mRNA metabolic process
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0035403 histone kinase activity (H3-T6 specific)
GO:0048011 nerve growth factor receptor signaling pathway
GO:0070555 response to interleukin-1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"