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Protein Kinase C Alpha antibody | 133

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Anti Protein Kinase C Alpha Antibody, clone 133 gallery image 1
Western Blot analysis of Protein Kinase C alpha 25 μg of A549, Hepg2 and K562 were loaded in to lanes 2, 3 and 4 respectively. Cell lysates were run under reducing conditions and transferred onto a nitrocellulose membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. Mouse anti Protein Kinase C alpha was used as the primary antibody at a dilution of 1/1000 in lanes 1-4. Goat anti Mouse IgG conjugated to HRP (STAR207P) was used as the secondary reagent at 1/10000. Membranes were visualized on the ChemiDoc MP Imaging System

Mouse anti Protein Kinase C Alpha

Product Type
Monoclonal Antibody
Clone
133
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1572
Datasheet
C WB
MSDS
2 ml loader

Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the α isoform of protein kinase C. PKC-α is a 672 amino acid enzyme containng a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain (UniProt: P17252).

Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the sequence PQFVHPILQSAV at the C terminus of PKCα.

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Protein kinase C alpha.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P17252 Related reagents
Entrez Gene
PRKCA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006112 energy reserve metabolic process
GO:0007202 activation of phospholipase C activity
GO:0050796 regulation of insulin secretion
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016071 mRNA metabolic process
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0035403 histone kinase activity (H3-T6 specific)
GO:0048011 nerve growth factor receptor signaling pathway
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of Protein Kinase C Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat 1/10
Western Blotting 1/10 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Protein Kinase C Alpha antibody

  1. Donovan, A.J. et al. (2012) Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
    Mol Pharmacol. 82 (3): 428-37.
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