Protein Kinase C Alpha antibody | 133
Mouse anti Protein Kinase α antibody, clone 133 (MCA1572) used for the demonstration of PKCα in murine retinal tissue by immunofluorescence.
Image caption:
GLE delayed the differentiation of Chx10-IR and PKCα-IR BCs. The developing retinas from (A–J) the control and (K–T) GLE mice were double labeled with antibodies against Chx10 (green: A–E and K–O) and PKCα (red: F–J and P–T) A: In the PN3 controls, Chx10 labeled both the proliferative and the newly differentiated BCs in the INL, as denoted by diffuse and intense labelin,g respectively, as well as cells in the GCL. B: At PN5 and (C) PN7, Chx10 labeled differentiated BCs in the INL as well as cells in the GCL. For confirmation, Figure 8 shows Chx10 and CCND1, a cell cycle marker, labeling at PN1 and PN5. At PN7, a well-defined OPL was visible. D and E: The patterns at PN10 and PN60 was similar to that at PN7. K: In contrast, in the PN3 GLE retinas, Chx10 labeled only proliferative cells, and no differentiated Chx10-IR BCs were present. L: At PN5, Chx10-IR BCs were present, although they were spindle-shaped compared to the age-matched controls. M: At PN7, the patterns were similar in the control and GLE retinas. N and O: At PN10 and PN60, there were significantly more Chx10-IR cells in the GLE retinas, as described [21]. F: In the PN3 control retinas, PKCα-IR was in ISs, the IPL, and the GCL. G: At PN5, PKCα-IR increased in the ISs, IPL, and GCL and appeared in the OPL. H: At PN7, PKCα-IR was selectively and intensely expressed in the rod BCs, from their dendrites in the OPL and to their axon terminals in the most proximal INL. I: At PN10, the rod BCs continued to develop, as evidenced by the increased extent of the PKCα-IR. J: At PN60, PKCα-IR labeled the complete mature rod BC. P: In the PN3 GLE retinas, PKCα-IR was less intense and more diffuse than in the age-matched controls, as seen in the ISs, IPL, and GCL. Q and R: At PN5 and PN7, the GLE retinas were similar to the controls. However, the OPL was less developed, and fewer PKCα-IR rod BCs were present. S: At PN10, the BCs in the GLE increased in their number and thickness. T: In PN60, the BC number and thickness increased in the GLE retinas relative to the age-matched controls, as described [21]. Thus, GLE delayed the appearance and localization of Chx10 and PKCα-IR (Table 3). Scale bar = 40 μm. GLE= Gestational lead exposure; IR = immunoreactive; BC = bipolar cell; PN = postnatal; INL = inner nuclear layer; GCL = ganglion cell layer; CCND1 = cyclin D1; OPL = outer plexiform layer; IPL = inner plexiform layer.
From: Chaney SY, Mukherjee S, Giddabasappa A, et al.
Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Protein Kinase C Alpha
- Product Type
- Monoclonal Antibody
- Clone
- 133
- Isotype
- IgG2a
- Specificity
- Protein Kinase C Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the α isoform of protein kinase C. PKC-α is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain (UniProt: P17252). Mouse anti Protein Kinase C alpha antibody, clone 133 recognizes the sequence PQFVHPILQSAV at the C terminus of PKCα. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue culture supernatant - liquid
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Protein kinase C alpha.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
Neat | 1/10 |
| Western Blotting | |
1/10 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Protein Kinase C Alpha antibody
-
Donovan, A.J. et al. (2012) Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
Mol Pharmacol. 82 (3): 428-37. -
Chaney, S.Y. et al. (2016) Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation.
Mol Vis. 22: 1468-89.
- RRID
- AB_2284228
- UniProt
- P17252
- Entrez Gene
- PRKCA
- GO Terms
- GO:0005886 plasma membrane
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006112 energy reserve metabolic process
- GO:0007202 activation of phospholipase C activity
- GO:0050796 regulation of insulin secretion
- GO:0008270 zinc ion binding
- View More GO Terms
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0016071 mRNA metabolic process
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0035403 histone kinase activity (H3-T6 specific)
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0070555 response to interleukin-1
MCA1572
151112
163058
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