SLA Class II DQ antibody | K274.3G8

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Mouse anti Pig SLA Class II DQ

Product Type
Monoclonal Antibody
Clone
K274.3G8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1335GA C F P 0.1 mg
Mouse anti Pig SLA Class II DQ antibody, clone K274.3G8 recognizes SLA DQ molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3-X63-Ag.653 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of SLA Class II DQ antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for SLA Class II DQ antibody

  1. Lunney, J.K. (1993) Characterization of swine leukocyte differentiation antigens.
    Immunol Today 14: 147-8.
  2. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
    Vet Immunol Immunopathol. 64: 1-13.
  3. Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.
    Vet Pathol. 40: 395-404.
  4. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88: 571-8.
  5. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
    J Pharmacol Exp Ther. 302: 861-70.
  6. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
    Kidney Int. 62: 654-67.
  7. Paillot, R. et al. (2001) Functional and phenotypic characterization of distinct porcine dendritic cells derived from peripheral blood monocytes.
    Immunology 102: 396-404.
  8. Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
    Invest Ophthalmol Vis Sci. 43: 1488-92.
  9. Jayle, C. et al. (2007) Comparison of protective effects of trimetazidine against experimental warm ischemia of different durations: early and long-term effects in a pig kidney model.
    Am J Physiol Renal Physiol. 292: F1082-93.
  10. Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
    J Microbiol Biotechnol. 18: 1709-16.
  11. Maasilta, P.K. et al. (2005) Immune cells in a heterotopic lamb-to-pig bronchial xenograft model.
    Transpl Int. 18: 1100-8.
  12. Weesendorp E et al. (2013) Phenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strains in vitro and in vivo.
    Vet Microbiol. 167 (3-4): 638-50.
  13. Makala, L.H. et al. (2001) Ontogeny of pig discrete Peyer's patches: expression of surface antigens.
    J Vet Med Sci. 63 (6): 625-36.
  14. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.
    Immunology. 129 (3): 396-405.
  15. Edamura, K. et al. (2005) Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells.
    Xenotransplantation. 12 (4): 327-32.
  16. Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.
    Eur J Dermatol. 23 (4): 456-66.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.