SLA Class I antibody | JM1E3
Mouse anti Pig SLA Class I antibody, clone JM1E3 (MCA2261GA) used for the evaluation of SLA Class I expression on bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Effect of pericardial fluid on pBM-MSCs phenotype. The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean ±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.
From: Citation: Blázquez R, Sánchez-Margallo FM, Crisístomo V, Báez C, Maestre J, García-Lindo M, et al. (2015)
Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis.
PLoS ONE 10(3): e0122377.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Pig SLA Class I antibody, clone JM1E3 (MCA2261GA) used for the evaluation of SLA Class I expression in cells over-expressing β2-microglobulin by immunofluorescence.
Image caption:
Immunohistochemical analysis of the β2M and SLA class I expression in cells over-expressing β2M. The names of the transfected constructs are shown on top: pCMV-HA-B2M for the expression of HA-tagged β2M, pCMV-HA for the HA tag without β2M, and pEGFP-N1 for EGFP expression. The primary antibodies used for the analysis are shown on the left. A and B. Cells were stained with the HA tag-specific antibody. The cell nuclei were visualized using 4′,6-diamidino-2-phenylindole (DAPI; blue). C. pEGFP (green) plasmid was used to evaluate the transfection efficiency. D, E, and F. SLA class I heavy chains were stained with pan SLA class I-specific antibodies. The signals of primary antibodies were detected by Alexa 568-conjugated secondary antibody (red) in all cases.
From: Le TM, Le QVC, Truong DM, Lee H-J, Choi M-K, Cho H, et al. (2017)
β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS ONE 12(8): e0182322.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
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F IF ResetMouse anti Pig SLA Class I
- Product Type
- Monoclonal Antibody
- Clone
- JM1E3
- Isotype
- IgG1
- Specificity
- SLA Class I
Quick Links:
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| Mouse anti Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galiani et al. 2002) The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for SLA Class I antibody
-
Galiani, D. et al.. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors.
In Leucocyte Typing VII. Edited by Mason. D. et al.. Oxford University Press pp 437-39. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M
and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. -
Hurtado, C. et al. (2011) The African swine fever virus lectin EP153R modulates the surface membrane expression of MHC class I antigens.
Arch Virol. 156: 219-34. -
Ding, G. et al. (2010) Suppression of T cell proliferation by root apical papilla stem cells in vitro.
Cells Tissues Organs. 191: 357-64. -
Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
J Microbiol Biotechnol. 18: 1709-16. -
Van Parys, A. et al. (2012) Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages.
Vet Res. 43: 52. -
Löndt, B.Z. et al. (2013) Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organ cultures following adaptation by in vitro passage.
Virus Res. 178(2):383-91. -
Blázquez, R. et al. (2015) Intrapericardial administration of mesenchymal stem cells in a large animal model: a bio-distribution analysis.
PLoS One. 10 (3): e0122377.
View The Latest Product References
-
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Suarez-Pinzon, W. et al. (2015) A Novel Protocol for Culturing Adult Porcine Islets for Transplantation in Type 1 Diabetic Patients
Minn Acad Sci J Student Res.3: 1-11. -
Richmond, O. et al. (2015) PD-L1 expression is increased in monocyte derived dendritic cells in response to porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus infections.
Vet Immunol Immunopathol. 168 (1-2): 24-9. -
Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
Transpl Immunol. 32 (2): 99-108. -
Park, K.M. et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
Vet Res Commun. 37 (4): 293-301. -
Le, T.M. et al. (2017) β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS One. 12 (8): e0182322. -
Linard, C. et al. (2018) Autologous Bone Marrow Mesenchymal Stem Cells Improve the Quality and Stability of Vascularized Flap Surgery of Irradiated Skin in Pigs.
Stem Cells Transl Med. 7 (8): 569-582.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- UniProt
- O19244
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