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SLA Class I antibody | JM1E3

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Anti Pig SLA Class I Antibody, clone JM1E3 thumbnail image 1 Anti Pig SLA Class I Antibody, clone JM1E3 thumbnail image 2 Anti Pig SLA Class I Antibody, clone JM1E3 thumbnail image 3
Anti Pig SLA Class I Antibody, clone JM1E3 gallery image 1
Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine SLA 1:FITC (MCA2261F).
Anti Pig SLA Class I Antibody, clone JM1E3 gallery image 2
Published customer image:
Mouse anti Pig SLA Class I antibody, clone JM1E3 (MCA2261GA) used for the evaluation of SLA Class I expression on bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Effect of pericardial fluid on pBM-MSCs phenotype. The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean ±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.

From: Citation: Blázquez R, Sánchez-Margallo FM, Crisístomo V, Báez C, Maestre J, García-Lindo M, et al. (2015)
Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis.
PLoS ONE 10(3): e0122377.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Pig SLA Class I Antibody, clone JM1E3 gallery image 3
Published customer image:
Mouse anti Pig SLA Class I antibody, clone JM1E3 (MCA2261GA) used for the evaluation of SLA Class I expression in cells over-expressing β2-microglobulin by immunofluorescence.
Image caption:
Immunohistochemical analysis of the β2M and SLA class I expression in cells over-expressing β2M. The names of the transfected constructs are shown on top: pCMV-HA-B2M for the expression of HA-tagged β2M, pCMV-HA for the HA tag without β2M, and pEGFP-N1 for EGFP expression. The primary antibodies used for the analysis are shown on the left. A and B. Cells were stained with the HA tag-specific antibody. The cell nuclei were visualized using 4′,6-diamidino-2-phenylindole (DAPI; blue). C. pEGFP (green) plasmid was used to evaluate the transfection efficiency. D, E, and F. SLA class I heavy chains were stained with pan SLA class I-specific antibodies. The signals of primary antibodies were detected by Alexa 568-conjugated secondary antibody (red) in all cases.

From: Le TM, Le QVC, Truong DM, Lee H-J, Choi M-K, Cho H, et al. (2017)
β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS ONE 12(8): e0182322.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Mouse anti Pig SLA Class I:Alexa Fluor® 647

Mouse anti Pig SLA Class I:FITC

Mouse anti Pig SLA Class I

Product Type
Monoclonal Antibody
Clone
JM1E3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2261A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2261F
Datasheet
F
SDS
0.1 mg loader

MCA2261GA
Datasheet
F IF IP
SDS
0.1 mg loader

Mouse anti Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galiani et al. 2002)

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region.
Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002).

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of SLA Class I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for SLA Class I antibody

  1. Galiani, D. et al.. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors.
    In Leucocyte Typing VII. Edited by Mason. D. et al.. Oxford University Press pp 437-39.
  2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Clin Vaccine Immunol. 17: 503-12.
  3. Hurtado, C. et al. (2011) The African swine fever virus lectin EP153R modulates the surface membrane expression of MHC class I antigens.
    Arch Virol. 156: 219-34.
  4. Ding, G. et al. (2010) Suppression of T cell proliferation by root apical papilla stem cells in vitro.
    Cells Tissues Organs. 191: 357-64.
  5. Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
    J Microbiol Biotechnol. 18: 1709-16.
  6. Van Parys, A. et al. (2012) Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages.
    Vet Res. 43: 52.
  7. Löndt, B.Z. et al. (2013) Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organ cultures following adaptation by in vitro passage.
    Virus Res. 178(2):383-91.
  8. Blázquez, R. et al. (2015) Intrapericardial administration of mesenchymal stem cells in a large animal model: a bio-distribution analysis.
    PLoS One. 10 (3): e0122377.
  9. Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. 23 (1): 38-45.
  10. Suarez-Pinzon, W. et al. (2015) A Novel Protocol for Culturing Adult Porcine Islets for Transplantation in Type 1 Diabetic Patients
    Minn Acad Sci J Student Res.3: 1-11.
  11. Richmond, O. et al. (2015) PD-L1 expression is increased in monocyte derived dendritic cells in response to porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus infections.
    Vet Immunol Immunopathol. 168 (1-2): 24-9.
  12. Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
    Transpl Immunol. 32 (2): 99-108.
  13. Park, K.M. et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
    Vet Res Commun. 37 (4): 293-301.
  14. Le, T.M. et al. (2017) β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
    PLoS One. 12 (8): e0182322.
  15. Linard, C. et al. (2018) Autologous Bone Marrow Mesenchymal Stem Cells Improve the Quality and Stability of Vascularized Flap Surgery of Irradiated Skin in Pigs.
    Stem Cells Transl Med. 7 (8): 569-582.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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