SLA Class I antibody | JM1E3
Mouse anti Pig SLA Class I antibody, clone JM1E3 used for the evaluation of SLA Class I expression on bone marrow derived mesenchymal stem cells by flow cytometry.
Image caption:
Effect of pericardial fluid on pBM-MSCs phenotype. The phenotypic analysis was performed by multicolor flow cytometry. The cells were in vitro cultured for 7 days in the presence of Fetal Bovine Serum and in the presence of pericardial fluid at 25%, 50%, 75% and 100%. Representative histograms of four different experiments are shown (A). The expression level of Stem Cell Markers (CD29, CD31, CD44, CD90, CD105) and Swine Leukocyte Antigen Class-I and Class-II (SLA-I and SLA-II) is represented as Normalized Mean Relative Fluorescence Intensity which is calculated by dividing the Mean Fluorescent Intensity (MFI) by its isotype control. Graphic representation of mean±SD for each marker is also provided (n = 4) (B). No significant differences were found between groups when ANOVA test was performed.
From: Citation: Blázquez R, Sánchez-Margallo FM, Crisístomo V, Báez C, Maestre J, García-Lindo M, et al. (2015) Intrapericardial Administration of Mesenchymal Stem Cells in a Large Animal Model: A Bio-Distribution Analysis. PLoS ONE 10(3): e0122377.
Mouse anti Pig SLA Class I antibody, clone JM1E3 used for the evaluation of SLA Class I expression in cells over-expressing β2-microglobulin by immunofluorescence.
Image caption:
Immunohistochemical analysis of the β2M and SLA class I expression in cells over-expressing β2M. The names of the transfected constructs are shown on top: pCMV-HA-B2M for the expression of HA-tagged β2M, pCMV-HA for the HA tag without β2M, and pEGFP-N1 for EGFP expression. The primary antibodies used for the analysis are shown on the left. A and B. Cells were stained with the HA tag-specific antibody. The cell nuclei were visualized using 4′,6-diamidino-2-phenylindole (DAPI; blue). C. pEGFP (green) plasmid was used to evaluate the transfection efficiency. D, E, and F. SLA class I heavy chains were stained with pan SLA class I-specific antibodies. The signals of primary antibodies were detected by Alexa 568-conjugated secondary antibody (red) in all cases.
From: Le TM, Le QVC, Truong DM, Lee H-J, Choi M-K, Cho H, et al. (2017)
β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS ONE 12(8): e0182322.
Mouse anti Pig SLA Class I antibody, clone JM1E3 recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA - 1). SLA - 1 is expressed by all nucleated porcine cells, but not on erythrocytes. This antibody has also been shown to cross-react with human MHC Class I, including HLA-E. (Galiani et al. 2002)
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region.
Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002).
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region.
Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002).
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of SLA Class I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for SLA Class I antibody
-
Galiani, D. et al.. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors.
In Leucocyte Typing VII. Edited by Mason. D. et al.. Oxford University Press pp 437-39. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M
and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. -
Hurtado, C. et al. (2011) The African swine fever virus lectin EP153R modulates the surface membrane expression of MHC class I antigens.
Arch Virol. 156: 219-34. -
Ding, G. et al. (2010) Suppression of T cell proliferation by root apical papilla stem cells in vitro.
Cells Tissues Organs. 191: 357-64. -
Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
J Microbiol Biotechnol. 18: 1709-16. -
Van Parys, A. et al. (2012) Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages.
Vet Res. 43: 52. -
Löndt, B.Z. et al. (2013) Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organ cultures following adaptation by in vitro passage.
Virus Res. 178(2):383-91. -
Blázquez R et al. (2015) Intrapericardial administration of mesenchymal stem cells in a large animal model: a bio-distribution analysis.
PLoS One. 10 (3): e0122377. -
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Suarez-Pinzon, W. et al. (2015) A Novel Protocol for Culturing Adult Porcine Islets for Transplantation in Type 1 Diabetic Patients
Minn Acad Sci J Student Res.3: 1-11. -
Richmond, O. et al. (2015) PD-L1 expression is increased in monocyte derived dendritic cells in response to porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus infections.
Vet Immunol Immunopathol. 168 (1-2): 24-9. -
Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
Transpl Immunol. 32 (2): 99-108. -
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Park KM et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
Vet Res Commun. 37 (4): 293-301. -
Le, T.M. et al. (2017) β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS One. 12 (8): e0182322.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
