SLA Class I antibody | JM1E3



The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region.
Mouse anti pig SLA class I, clone JM1E3 has been reported to block the interaction of MHC Class I antigens with inhibitory NK cell receptors (Galiani et al. 2002).
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of SLA Class I antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 | 1/100 | |
Immunofluorescence | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Product Specific References
References for SLA Class I antibody
-
Galiani, D. et al.. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors.
In Leucocyte Typing VII. Edited by Mason. D. et al.. Oxford University Press pp 437-39. -
Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M
and N proteins of porcine reproductive and respiratory syndrome virus.
Clin Vaccine Immunol. 17: 503-12. -
Hurtado, C. et al. (2011) The African swine fever virus lectin EP153R modulates the surface membrane expression of MHC class I antigens.
Arch Virol. 156: 219-34. -
Ding, G. et al. (2010) Suppression of T cell proliferation by root apical papilla stem cells in vitro.
Cells Tissues Organs. 191: 357-64. -
Park, J.Y. et al. (2008) Characterization of interaction between porcine reproductive and respiratory syndrome virus and porcine dendritic cells.
J Microbiol Biotechnol. 18: 1709-16. -
Van Parys, A. et al. (2012) Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages.
Vet Res. 43: 52. -
Löndt, B.Z. et al. (2013) Enhanced infectivity of H5N1 highly pathogenic avian influenza (HPAI) virus in pig ex vivo respiratory tract organ cultures following adaptation by in vitro passage.
Virus Res. 178(2):383-91. -
Blázquez, R. et al. (2015) Intrapericardial administration of mesenchymal stem cells in a large animal model: a bio-distribution analysis.
PLoS One. 10 (3): e0122377. -
Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45. -
Suarez-Pinzon, W. et al. (2015) A Novel Protocol for Culturing Adult Porcine Islets for Transplantation in Type 1 Diabetic Patients
Minn Acad Sci J Student Res.3: 1-11. -
Richmond, O. et al. (2015) PD-L1 expression is increased in monocyte derived dendritic cells in response to porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus infections.
Vet Immunol Immunopathol. 168 (1-2): 24-9. -
Iwase H et al. (2015) Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.
Transpl Immunol. 32 (2): 99-108. -
Park, K.M. et al. (2013) Generation of porcine induced pluripotent stem cells and evaluation of their major histocompatibility complex protein expression in vitro.
Vet Res Commun. 37 (4): 293-301. -
Le, T.M. et al. (2017) β2-microglobulin gene duplication in cetartiodactyla remains intact only in pigs and possibly confers selective advantage to the species.
PLoS One. 12 (8): e0182322. -
Linard, C. et al. (2018) Autologous Bone Marrow Mesenchymal Stem Cells Improve the Quality and Stability of Vascularized Flap Surgery of Irradiated Skin in Pigs.
Stem Cells Transl Med. 7 (8): 569-582.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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