Na+/H+ Exchanger-1 antibody | 4E9

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Mouse anti Na+/H+ Exchanger-1

Product Type
Monoclonal Antibody
Clone
4E9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1655 WB 0.1 mg
Mouse anti Porcine sodium/hydrogen exchanger 1 antibody, clone 4E9 recognizes the Na+/H+ exchanger-1 (NHE1), a membrane protein involved in pH regulation and signal transduction.
Mouse anti Porcine sodium/hydrogen exchanger 1 antibody, clone 4E9 recognizes NHE1 from the salamander Amphiuma tridactylum (McLean et al. 1999) and in the flounder Pseudopleuronectes americanus

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Vertebrates Expected from Sequence
Rabbit
Fish
Mouse
Rat
Salamander
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Maltose binding protein fusion protein containing the entire C-terminal, hydrophilic domain of porcine NHE1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P48762 Related reagents
Entrez Gene
SLC9A1 Related reagents
GO Terms
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of Na+/H+ Exchanger-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
OBT1655 detects a band of approximately 100 kDa in human kidney lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for Na+/H+ Exchanger-1 antibody

  1. Rutherford, P.A. et al. (1997) Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
    Exp Nephrol. 5 (6): 490-7.
  2. Choe, K.P. et al. (2002) Immunological detection of Na(+)/H(+) exchangers in the gills of a hagfish, Myxine glutinosa, an elasmobranch, Raja erinacea, and a teleost, Fundulus heteroclitus.
    Comp Biochem Physiol A Mol Integr Physiol. 131: 375-85.
  3. Claiborne, J.B. et al. (1999) A mechanism for branchial acid excretion in marine fish: identification of multiple Na+/H+ antiporter (NHE) isoforms in gills of two seawater teleosts.
    J Exp Biol. 202: 315-24.
  4. Biemesderfer, D. et al. (1999) Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule.
    J Biol Chem. 274 (25): 17518-24.
  5. McLean LA et al. (1999) Cloning and expression of the Na+/H+ exchanger from Amphiuma RBCs: resemblance to mammalian NHE1.
    Am J Physiol. 276 (5 Pt 1): C1025-37.
  6. Pedersen, S.F. et al. (2003) Molecular cloning of NHE1 from winter flounder RBCs: activation by osmotic shrinkage, cAMP, and calyculin A.
    Am J Physiol Cell Physiol. 284 (6): C1561-76.
  7. Liu, F. & Gesek, F.A. (2001) alpha(1)-Adrenergic receptors activate NHE1 and NHE3 through distinct signaling pathways in epithelial cells.
    Am J Physiol Renal Physiol. 280 (3): F415-25.
  8. Goyal, S. et al. (2003) Renal expression of novel Na+/H+ exchanger isoform NHE8.
    Am J Physiol Renal Physiol. 284 (3): F467-73.