Na+/H+ Exchanger-1 antibody | 4E9
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Mouse anti Na+/H+ Exchanger-1
- Product Type
- Monoclonal Antibody
- Clone
- 4E9
- Isotype
- IgG1
- Specificity
- Na+/H+ Exchanger-1
Antibody Quality and Performance Guarantee
| Mouse anti Porcine sodium/hydrogen exchanger 1 antibody, clone 4E9 recognizes the Na+/H+ exchanger-1 (NHE1), a membrane protein involved in pH regulation and signal transduction. Mouse anti Porcine sodium/hydrogen exchanger 1 antibody, clone 4E9 recognizes NHE1 from the salamander Amphiuma tridactylum (McLean et al. 1999) and in the flounder Pseudopleuronectes americanus |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Vertebrates Expected from Sequence Rabbit Fish Mouse Rat Salamander - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Maltose binding protein fusion protein containing the entire C-terminal, hydrophilic domain of porcine NHE1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting |  |
1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- OBT1655 detects a band of approximately 100 kDa in human kidney lysates.
References for Na+/H+ Exchanger-1 antibody
-
Rutherford, P.A. et al. (1997) Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Exp Nephrol. 5 (6): 490-7. -
McLean LA et al. (1999) Cloning and expression of the Na+/H+ exchanger from Amphiuma RBCs: resemblance to mammalian NHE1.
Am J Physiol. 276 (5 Pt 1): C1025-37. -
Biemesderfer, D. et al. (1999) Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule.
J Biol Chem. 274 (25): 17518-24. -
Claiborne, J.B. et al. (1999) A mechanism for branchial acid excretion in marine fish: identification of multiple Na+/H+ antiporter (NHE) isoforms in gills of two seawater teleosts.
J Exp Biol. 202: 315-24. -
Liu, F. & Gesek, F.A. (2001) alpha(1)-Adrenergic receptors activate NHE1 and NHE3 through distinct signaling pathways in epithelial cells.
Am J Physiol Renal Physiol. 280 (3): F415-25. -
Choe, K.P. et al. (2002) Immunological detection of Na(+)/H(+) exchangers in the gills of a hagfish, Myxine glutinosa, an elasmobranch, Raja erinacea, and a teleost, Fundulus heteroclitus.
Comp Biochem Physiol A Mol Integr Physiol. 131: 375-85. -
Goyal, S. et al. (2003) Renal expression of novel Na+/H+ exchanger isoform NHE8.
Am J Physiol Renal Physiol. 284 (3): F467-73. -
Pedersen, S.F. et al. (2003) Molecular cloning of NHE1 from winter flounder RBCs: activation by osmotic shrinkage, cAMP, and calyculin A.
Am J Physiol Cell Physiol. 284 (6): C1561-76.
View The Latest Product References
- RRID
- AB_609778
- UniProt
- P48762
- Entrez Gene
- SLC9A1
- GO Terms
- GO:0016021 integral to membrane
OBT1655
145796
149681
166354
166535
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