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IL-1 Beta antibody | DF8

Mouse anti Pig Interleukin-1 beta:Preservative Free

Product Type
Monoclonal Antibody
Clone
DF8
Isotype
IgG1
Specificity
IL-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA2361Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.5 mg loader
List Price Your Price
loader

Mouse anti Porcine interleukin-1 beta antibody, clone DF8 recognizes porcine interleukin-1 beta (IL-1 beta).

IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the porcine immune response.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant porcine IL-1 beta.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Endotoxin Level
< 0.015 EU/μg
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for IL-1 Beta antibody

  1. Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
    Vet Immunol Immunopathol. 124: 367-78.
  2. Shao, Y. et al. (2016) HMGB1/TLR4 signaling induces an inflammatory response following high-pressure renal pelvic perfusion in a porcine model.
    Am J Physiol Renal Physiol. : ajprenal.00480.2015.
  3. Jarosova, R. et al. (2022) Cytokine expression by CD163+ monocytes in healthy and Actinobacillus pleuropneumoniae-infected pigs.
    Res Vet Sci. 152: 1-9.

RRID
AB_567066
UniProt
P26889
Entrez Gene
IL1B
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division

MCA2361Z

161007 166849

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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