CD61 antibody | JM2E5
Mouse anti Pig CD61 antibody, clone JM2E5 (MCA2263) used for evaluation of expression of CD61 on bovine epithelial cells by flow cytometry.
Image caption:
Determination of receptor distribution on bovine epithelial cells.
Uninfected epithelial cell (Pf [panel A], G [B], U [C]) were analyzed by flow cytometry for expression of CR3 and αVβ3 on their surface. Grey shaded curves depict detection of the respective antigens and black lines represent secondary antibody control (representative results of two technical replicates in two independent experiments).
From: Sobotta K, Bonkowski K, Liebler-Tenorio E, et al.
Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.
Vet Res. 2017;48(1):23.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa or integrin beta.
CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002).
The epitope recognized by this antibody is not sensitive to EDTA.
Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting.
CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002).
The epitope recognized by this antibody is not sensitive to EDTA.
Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting.
Product Details
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Human Bovine Horse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q95JH1
- Regulatory
- For research purposes only
Applications of CD61 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting |
- 1 This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD61 antibody
-
Pérez de la Lastra, J.M. et al. (1997) Characterization of the porcine homologue to human platelet glycoprotein IIb-IIIa (CD41/CD61) by a monoclonal antibody.
Tissue Antigens. 49 (6): 588-94. -
Arce, C et al. (2001) Expression of CD61 (beta 3 integrin subunit) on canine cells.
Platelets 12:69-73. -
Moreno, A. et al. (2002) Immunohistochemical analysis of beta3 integrin (CD61): expression in pig tissues and human tumors.
Histol Histopathol. 17 (2): 347-52. -
Zhang, J.L. et al. (2007) Up-regulated expression of beta3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvascular endothelial cells.
Biochem Biophys Res Commun. 356: 763-8. -
Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line.
Vet Res. 35: 625-33. -
Sobotta, K. et al. (2017) Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.
Vet Res. 48 (1): 23.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
