CD61 antibody | JM2E5

Mouse anti Pig CD61

Product Type
Monoclonal Antibody
Clone
JM2E5
Isotype
IgG1
Specificity
CD61

Product Code Applications Pack Size List Price Your Price Qty
MCA2263GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * WB 0.1 mg loader
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loader
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Mouse anti Pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa or integrin beta.

CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002).

Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting. The epitope recognized by this antibody is not sensitive to EDTA.

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Human
Bovine
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD61 antibody

  1. Pérez de la Lastra, J.M. et al. (1997) Characterization of the porcine homologue to human platelet glycoprotein IIb-IIIa (CD41/CD61) by a monoclonal antibody.
    Tissue Antigens. 49 (6): 588-94.
  2. Arce, C et al. (2001) Expression of CD61 (beta 3 integrin subunit) on canine cells.
    Platelets 12:69-73.
  3. Moreno, A. et al. (2002) Immunohistochemical analysis of beta3 integrin (CD61): expression in pig tissues and human tumors.
    Histol Histopathol. 17 (2): 347-52.
  4. Zhang, J.L. et al. (2007) Up-regulated expression of beta3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvascular endothelial cells.
    Biochem Biophys Res Commun. 356: 763-8.
  5. Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line.
    Vet Res. 35: 625-33.
  6. Sobotta, K. et al. (2017) Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.
    Vet Res. 48 (1): 23.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
Integrin Beta 3 Chain
RRID
AB_11152935
UniProt
Q95JH1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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