Mouse anti Pig CD45 allotypic variant antibody, clone MAC323
recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). The antibody has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4
, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve
animals rather than loss of the CD45 antigen. Cross-breeding studies in positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binns et al. 1995
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Porcine peripheral blood lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD45 antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
|Immunohistology - Resin 1
- 1This product requires antigen retrieval using heat treatment of resin sections. This product does not require antigen retrieval prior to staining paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Application Based External Images
Immunohistology - Paraffin
Immunohistology - Resin
Product Specific References
References for CD45 antibody
Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.
Immunology 86: 25-33.
Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.
Cardiovasc Revasc Med. 7: 136-45.
Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.
Pediatr Surg Int. 19: 326-30.
Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.
J Clin Invest. 113: 1607-14.
Kozian, A. et al. (2010) Increased alveolar damage after mechanical ventilation in a porcine model of thoracic surgery.
J Cardiothorac Vasc Anesth. 24 (4): 617-23.
Nugent, H.M. et al. (2012) Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.
J Vasc Surg. 56 (4): 1078-88.
VanDitzhuijzen, N.S. et al. (2017) Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
PLoS One. 12 (9): e0183419.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.