CD45 antibody | MAC323
Mouse anti Porcine CD45 antibody, clone MAC323 (MCA1447) used to illustrate inflammatory cells in an implant model by immunohistochemistry
Image caption:
CD45 histology at 6M.
OCT demonstrated the development of a highly heterogeneous neointima with inflammation (F; CD45).
From: van Ditzhuijzen NS, Kurata M, van den Heuvel M, Sorop O, van Duin RWB, Krabbendam-Peters I, et al. (2017)
Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
PLoS ONE 12(9): e0183419.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD45 antibody, clone MAC323 (MCA1447) used to illustrate inflammatory cells in an implant model by immunohistochemistry on cryostat sections.
Image caption:
Collagen poor areas in peri-strut regions and neointima showing leucocyte infiltration (C, F; CD45).
From: van Ditzhuijzen NS, Kurata M, van den Heuvel M, Sorop O, van Duin RWB, Krabbendam-Peters I, et al. (2017)
Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.PLoS ONE 12(9): e0183419.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD45 antibody, clone MAC323 (MCA1447GA) used to identify leukocytes in porcine testis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical labeling of leukocytes (CD45 positive in A,B) in the testis. Arrows point to individual leukocytes in testis from a 6.5-week-old boar in (A), from a 20-week-old boar in (B). Inset is a normal serum control and is similar to the negatives for the other treatment and age. Scale bar is 50 μm.
From: Katleba K, Legacki E, Berger T.
Expression of CSF1, AR, and SRD5A2 during Postnatal Development of the Boar Reproductive Tract.
Animals (Basel). 2022 Aug 24;12(17):2167.
doi: 10.3390/ani12172167.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD45 antibody, clone MAC323 (MCA1447GA) used to identify leukocytes in porcine testis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical localization of leukocytes (A,C,E,G) in the prostate during development. (A) a section from prostate from 2-week-old boars; (C) prostate section from a6.5-week-old boar, (E) section from 11-week-old boar; (G) is a prostate section from a 40-week-old boar. Arrows point to individual cells. Inset is normal serum control and similar to the serum controls at other ages. Scale bar is 50 μm.
From: Katleba K, Legacki E, Berger T.
Expression of CSF1, AR, and SRD5A2 during Postnatal Development of the Boar Reproductive Tract.
Animals (Basel). 2022 Aug 24;12(17):2167.
doi: 10.3390/ani12172167.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD45 Allotypic Variant
- Product Type
- Monoclonal Antibody
- Clone
- MAC323
- Isotype
- IgG2a
- Specificity
- CD45
- Region
- ALLOTYPIC VARIANT
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binns et al. 1995). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine peripheral blood lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunohistology - Frozen | |
1/10 | |
| Immunohistology - Paraffin | |
1/10 | |
| Immunohistology - Resin 1 | |
1/10 | |
| Immunoprecipitation | |
- 1This product requires antigen retrieval using heat treatment of resin sections. This product does not require antigen retrieval prior to staining paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD3:FITC | MCA5951F | F | 0.1 mg |
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| Description | Mouse anti Pig CD3:FITC | ||||||
Source Reference
-
Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.
Immunology 86: 25-33.
References for CD45 antibody
-
Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.
Pediatr Surg Int. 19: 326-30. -
Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.
J Clin Invest. 113: 1607-14. -
Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.
Cardiovasc Revasc Med. 7: 136-45. -
Kozian, A. et al. (2010) Increased alveolar damage after mechanical ventilation in a porcine model of thoracic surgery.
J Cardiothorac Vasc Anesth. 24 (4): 617-23. -
Nugent, H.M. et al. (2012) Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.
J Vasc Surg. 56 (4): 1078-88. -
van Ditzhuijzen, N.S. et al. (2017) Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
PLoS One. 12 (9): e0183419. -
Katleba, K. et al. (2022) Expression of CSF1, AR, and SRD5A2 during Postnatal Development of the Boar Reproductive Tract.
Animals (Basel). 12 (17): 2167.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
MCA1447GA
163374
1806
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