CD45 antibody | MAC323
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|Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binns et al. 1995).|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Porcine peripheral blood lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/10|
|Immunohistology - Paraffin||1/10|
|Immunohistology - Resin 1||1/10|
- 1This product requires antigen retrieval using heat treatment of resin sections. This product does not require antigen retrieval prior to staining paraffin sections.
Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.
Immunology 86: 25-33.
References for CD45 antibody
Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.
Cardiovasc Revasc Med. 7: 136-45.
Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.
Pediatr Surg Int. 19: 326-30.
Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.
J Clin Invest. 113: 1607-14.
Kozian, A. et al. (2010) Increased alveolar damage after mechanical ventilation in a porcine model of thoracic surgery.
J Cardiothorac Vasc Anesth. 24 (4): 617-23.
Nugent, H.M. et al. (2012) Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.
J Vasc Surg. 56 (4): 1078-88.
van Ditzhuijzen, N.S. et al. (2017) Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
PLoS One. 12 (9): e0183419.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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