CD45 antibody | MAC323

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Mouse anti Pig CD45 Allotypic Variant

Product Type
Monoclonal Antibody
Clone
MAC323
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1447GA C F IP P RE* 0.1 mg
Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). The antibody has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding studies in positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binns et al. 1995).

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunohistology - Frozen 1/10
Immunohistology - Paraffin 1/10
Immunohistology - Resin 1 1/10
Immunoprecipitation
  1. 1This product requires antigen retrieval using heat treatment of resin sections. This product does not require antigen retrieval prior to staining paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig CD3:FITC MCA5951F 0.1 mg F

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Immunohistology - Resin

Product Specific References

References for CD45 antibody

  1. Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.
    Immunology 86: 25-33.
  2. Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs.
    Cardiovasc Revasc Med. 7: 136-45.
  3. Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis.
    Pediatr Surg Int. 19: 326-30.
  4. Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions.
    J Clin Invest. 113: 1607-14.
  5. Kozian, A. et al. (2010) Increased alveolar damage after mechanical ventilation in a porcine model of thoracic surgery.
    J Cardiothorac Vasc Anesth. 24 (4): 617-23.
  6. Nugent, H.M. et al. (2012) Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.
    J Vasc Surg. 56 (4): 1078-88.
  7. VanDitzhuijzen, N.S. et al. (2017) Neoatherosclerosis development following bioresorbable vascular scaffold implantation in diabetic and non-diabetic swine.
    PLoS One. 12 (9): e0183419.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
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