CD45 antibody | K252.1E4

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Mouse anti Pig CD45:Alexa Fluor® 647

Mouse anti Pig CD45:FITC

Mouse anti Pig CD45

Product Type
Monoclonal Antibody
Clone
K252.1E4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1222A647 F 100 Tests/1ml
MCA1222F F 100 Tests
MCA1222GA C F IF 0.1 mg
Mouse anti Pig CD45, clone K252.1E4 recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998). CD45 is also known as leukocyte common antigen (LCA).

Mouse anti Pig CD45, clone K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190 kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermann et al. 1994).

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine Peripheral Blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD45 antibody

  1. Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
    Invest Ophthalmol Vis Sci. 43 (5): 1488-92.
  2. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273-83.
  3. Barker, E. et al. (2006) The larynx as an immunological organ: immunological architecture in the pig as a large animal model.
    Clin Exp Immunol. 143: 6-14.
  4. Vilahur, G. et al. (2015) Roflumilast-induced Local Vascular Injury Is Associated with a Coordinated Proteome and Microparticle Change in the Systemic Circulation in Pigs.
    Toxicol Pathol. 43 (4): 569-80.
  5. O'Leary, S. et al. (2004) Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig.
    Reproduction. 128: 237-47.
  6. Zelnickova, P. et al. (2006) Postnatal functional maturation of blood phagocytes in pig.
    Vet Immunol Immunopathol. 113: 383-91.
  7. Bimczok, D. et al. (2006) Phenotype and distribution of dendritic cells in the porcine small intestinal and tracheal mucosa and their spatial relationship to epithelial cells.
    Cell Tissue Res. 325: 461-8.
  8. Nochi, T. et al. (2004) Biological role of Ep-CAM in the physical interaction between epithelial cells and lymphocytes in intestinal epithelium.
    Clin Immunol. 113: 326-39.
  9. Bimczok, D. et al. (2010) Primary porcine CD11R1+ antigen-presenting cells isolated from small intestinal mucosa mature but lose their T cell stimulatory function in response to cholera toxin treatment.
    Vet Immunol Immunopathol. 134: 239-48.
  10. Ebdrup, L. et al. (2008) Dynamic expression of the signal regulatory protein alpha and CD18 on porcine PBMC during acute endotoxaemia.
    Scand J Immunol. 68: 430-7.
  11. Plánka, L. et al. (2009) Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs.
    Physiol Res. 58: 885-93.
  12. Plánka, L. et al. (2009) Comparison of Preventive and Therapeutic Transplantations of Allogeneic Mesenchymal Stem Cells in Healing of the Distal Femoral Growth Plate Cartilage Defects in Miniature Pigs.
    Acta Vet. Brno 78: 293-302.
  13. Plánka, L. et al. (2008) New options for management of posttraumatic articular cartilage defects.
    Rozhl Chir. 87: 42-5.
  14. Splíchal, I. et al. (2002) Escherichia coli Administered into Pig Amniotic Cavity Appear in Fetal Airways and Attract Macrophages into Fetal Lungs.
    Physiol Res. 51: 523-8.
  15. Juhásová, J. et al. (2011) Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment.
    Physiol Res. 60: 559-71.
  16. Heino, T.J. et al. (2012) Comparison of the osteogenic capacity of minipig and human bone marrow-derived mesenchymal stem cells.
    J Orthop Res. 30: 1019-25.
  17. Stepanova, H. et al. (2012) CD4+ and γδTCR+ T lymphocytes are sources of interleukin-17 in swine.
    Cytokine. 58: 152-7.
  18. Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets.
    Clin Vaccine Immunol. 19: 1499-508.
  19. Noort, W.A. et al. (2011) Human versus porcine mesenchymal stromal cells: phenotype, differentiation potential, immunomodulation and cardiac improvement after transplantation.
    J Cell Mol Med. 16: 1827-39.
  20. Thorum, S.C. et al. (2013) Impact of diet on development of bronchial-associated immunity in the neonatal piglet.
    Vet Immunol Immunopathol. 151: 63-72.
  21. Tran, L.A. et al. (2010) Gadonanotubes as Magnetic Nanolabels for Stem Cell Detection
    Biomaterials. 31: 9482-91.
  22. Thorum, S.C. et al. (2013) Dietary (1,3/1,6)-β-D-glucan decreases transforming growth factor β expression in the lung of the neonatal piglet.
    Nutr Res. 33 (4): 322-31.
  23. Koskinas, K.C. et al. (2013) Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs.
    Int J Cardiol. 169 (6): 394-401.
  24. Franzoni, G. et al. (2013) Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge.
    Clin Vaccine Immunol. 20: 1604-16.
  25. Filová E, et al. (2009) Regionally-selective cell colonization of micropatterned surfaces prepared by plasma polymerization of acrylic acid and 1,7-octadiene.
    Physiol Res. 58: 669-84.
  26. Bimczok, D. et al. (2005) Site-specific expression of CD11b and SIRPalpha (CD172a) on dendritic cells: implications for their migration patterns in the gut immune system.
    Eur J Immunol. 35: 1418-27.
  27. Sun, Z. et al. (2013) Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model.
    PLoS One. 8: e74672.
  28. Post, I.C. et al. (2013) Characterization and quantification of porcine circulating endothelial cells.
    Xenotransplantation. 20: 18-26.
  29. Foubert, P. et al. (2015) Uncultured adipose-derived regenerative cells (ADRCs) seeded in collagen scaffold improves dermal regeneration, enhancing early vascularization and structural organization following thermal burns.
    Burns. 41 (7): 1504-16.
  30. Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
    Xenotransplantation. Feb 29. [Epub ahead of print]
  31. Antonson, A.M. et al. (2016) Maternal viral infection during pregnancy elicits anti-social behavior in neonatal piglet offspring independent of postnatal microglial cell activation.
    Brain Behav Immun. Sep 17. pii: S0889-1591(16)30428-7. [Epub ahead of print]
  32. Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
    J Diabetes Res. 2016: 3486727.
  33. Kaczmarek, M.M. et al. (2013) Seminal plasma affects prostaglandin synthesis and angiogenesis in the porcine uterus.
    Biol Reprod. 88 (3): 72.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.