CD203a antibody | PM18-7

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Anti Pig CD203a Antibody, clone PM18-7 thumbnail image 1

Anti Pig CD203a Antibody, clone PM18-7 thumbnail image 2

Anti Pig CD203a Antibody, clone PM18-7 thumbnail image 3

Anti Pig CD203a Antibody, clone PM18-7 thumbnail image 4

Anti Pig CD203a Antibody, clone PM18-7 gallery image 1 — Staining of pig alveolar macrophages with Mouse anti Pig CD203a (**MCA1973GA**) followed by Goat anti Mouse IgG:FITC (STAR117F)

Anti Pig CD203a Antibody, clone PM18-7 gallery image 2 — **Published customer image:**
Mouse anti Pig CD203a antibody, clone PM18-7 (**MCA1973GA**) used for the evaluation of CD203a expression on porcine leukocytes by flow cytometry.
**Image caption:**
Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203a^hi leukocytes (d) and marked with blue color. Monocytes were gated as CD203a^low/- SWC8^-(e) CD172a^hi(f) leukocytes where the CD203a^low/- region was defined as the complementary region to the CD203a^hi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.

From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental *Actinobacillus pleuropneumoniae* infection and the role of chemokines.
Vet Res. 2013 Oct 17;44:98.

Anti Pig CD203a Antibody, clone PM18-7 gallery image 3 — **Published customer image:**
Mouse anti Porcine CD203a antibody, clone PM18-7 (**MCA1973GA**) used to assess SWC9 expression on porcine dendritic cells following PRRSV infection by flow cytometry.
**Image caption:**
Single colour flow cytometry analysis of changes in several immunologically relevant cell surface molecules of bone marrow-derived dendritic cells after 48 h of incubation with different PRRSV strains at a multiplicity of infection of 0.01 (as determined by titration in porcine alveolar macrophages). Grey histograms show cells stained with an irrelevant isotype-matched antibody and a secondary FITC or phycoerythrin conjugated antibody. Blue histograms show uninfected cells and red line histograms correspond to PRRSV-infected cells. Averages and variation coefficients are included. Grey values show the uninfected cell parameters and black values correspond to PRRSV-infected cells. SwC3, SwC9, CD163, CD16.

From: Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E.
Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 2011 Jan 18;42:9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Pig CD203a Antibody, clone PM18-7 gallery image 4 — **Published customer image:**
FITC conjugated Mouse anti Porcine CD203a antibody, clone PM18/7 (**MCA1973F**) used as part of a panel to phenotype porcine peripheral blood derived macrophages by flow cytometry.
**Image caption:**
ΔSRCR5 peripheral blood monocyte-derived macrophages (PMMs) are fully differentiated and express macrophage-specific markers.
Peripheral blood monocytes were isolated from the blood of the wild type (red), heterozygous (blue), and ΔSRCR5 (green) animals. Following cultivation in the presence of recombinant human CSF1 (rhCSF1) for seven days PMMs were analyzed by FACS. A) Co-staining with CD14-FITC and CD16-PE antibodies recognizing the native structure of the proteins (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). B) Co-staining with CD169-FITC and CD172a-PE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). C) Co-staining with SWC9 (CD203a)-FITC and CD151-RPE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). D) Staining against the native structure of surface expressed CD163 (colored) relative to an isotype control staining (grey).

From: Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, *et al*. (2017)
Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog 13(2): e1006206.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License. **Published customer image:**

**Image caption:**
ΔSRCR5 peripheral blood monocyte-derived macrophages (PMMs) are fully differentiated and express macrophage-specific markers.
Peripheral blood monocytes were isolated from the blood of the wild type (red), heterozygous (blue), and ΔSRCR5 (green) animals. Following cultivation in the presence of recombinant human CSF1 (rhCSF1) for seven days PMMs were analyzed by FACS. A) Co-staining with CD14-FITC and CD16-PE antibodies recognizing the native structure of the proteins (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). B) Co-staining with CD169-FITC and CD172a-PE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). C) Co-staining with SWC9 (CD203a)-FITC and CD151-RPE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). D) Staining against the native structure of surface expressed CD163 (colored) relative to an isotype control staining (grey).

From: Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, *et al*. (2017)
Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog 13(2): e1006206.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Pig CD203a:FITC

Mouse anti Pig CD203a

Product Type: Monoclonal Antibody
Clone: PM18-7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1973F	F			0.1 mg
MCA1973GA	C F IP			0.1 mg

Mouse anti Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007).

Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of porcine alveolar macrophages.

CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015).

SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999).

Product Details

Target Species

Pig

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Porcine alveolar macrophages.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized mice were fused with P3X63-Ag8-653 murine myeloma cells (Kearney et al. 1979).

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch

More Information

Regulatory: For research purposes only

Applications of CD203a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry	1/25	1/200
Immunohistology - Frozen
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD203a antibody

McCullough, K.C. et al. (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.
Vet Immunol Immunopathol. 58 (3-4): 265-75.
McCullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology. 98 (2): 203-12.
Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80 (1-2): 63-78.
Domínguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60 (3-4): 329-41.
Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentiation antigen, SWC9.
Vet Immunol Immunopathol. 60 (3-4): 343-9.
Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies.
Dev Comp Immunol. 31 (6): 618-31.
Basta, S. et al. (1999) Modulation of monocytic cell activity and virus susceptibility during differentiation into macrophages.
J Immunol. 162 (7): 3961-9.
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9.
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7.
Cantu, E. et al. (2006) Depletion of pulmonary intravascular macrophages prevents hyperacute pulmonary xenograft dysfunction.
Transplantation. 81 (8): 1157-64.
Basta, S. et al. (2001) Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility.
Immunology. 103 (4): 488-97.
Lithgow, P. et al. (2014) Correlation of cell surface marker expression with African swine fever virus infection.
Vet Microbiol. 168: 413 - 9.
Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and< i> Salmonella-infected piglets by seven-colour flow cytometry.
Res Vet Sci. 94: 240 - 5.
Tsai, Y.C. et al. (2014) Differences in the expression of innate immune response-modulating genes in blood monocytes between subclinically porcine circovirus type s (PCV2)-infected and PCV2-free pigs prior to and after lipopolysaccharide stimulation in vitro
Taiwan Veterinary Journal. 40 (01): 37-48.
Hwang, J.H.et al. (2015) Characterization of monoclonal antibodies against porcine pulmonary alveolar macrophages of gnotobiotic miniature swine.
Biochem Biophys Res Commun. 461 (2): 427-34.
Shao, L. et al. (2016) Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs.
Vet Immunol Immunopathol. 171: 7-16.
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206.
Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
Dev Comp Immunol. 81: 95-104.
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92.