CD203a antibody | PM18-7
Mouse anti Pig CD203a antibody, clone PM18-7 (MCA1973GA) used for the evaluation of CD203a expression on porcine leukocytes by flow cytometry.
Image caption:
Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.
From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M.
Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines.
Vet Res. 2013 Oct 17;44:98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine CD203a antibody, clone PM18-7 (MCA1973GA) used to assess SWC9 expression on porcine dendritic cells following PRRSV infection by flow cytometry.
Image caption:
Single colour flow cytometry analysis of changes in several immunologically relevant cell surface molecules of bone marrow-derived dendritic cells after 48 h of incubation with different PRRSV strains at a multiplicity of infection of 0.01 (as determined by titration in porcine alveolar macrophages). Grey histograms show cells stained with an irrelevant isotype-matched antibody and a secondary FITC or phycoerythrin conjugated antibody. Blue histograms show uninfected cells and red line histograms correspond to PRRSV-infected cells. Averages and variation coefficients are included. Grey values show the uninfected cell parameters and black values correspond to PRRSV-infected cells. SwC3, SwC9, CD163, CD16.
From: Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E.
Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 2011 Jan 18;42:9.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Porcine CD203a antibody, clone PM18/7 (MCA1973F) used as part of a panel to phenotype porcine peripheral blood derived macrophages by flow cytometry.
Image caption:
ΔSRCR5 peripheral blood monocyte-derived macrophages (PMMs) are fully differentiated and express macrophage-specific markers.
Peripheral blood monocytes were isolated from the blood of the wild type (red), heterozygous (blue), and ΔSRCR5 (green) animals. Following cultivation in the presence of recombinant human CSF1 (rhCSF1) for seven days PMMs were analyzed by FACS. A) Co-staining with CD14-FITC and CD16-PE antibodies recognizing the native structure of the proteins (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). B) Co-staining with CD169-FITC and CD172a-PE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). C) Co-staining with SWC9 (CD203a)-FITC and CD151-RPE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). D) Staining against the native structure of surface expressed CD163 (colored) relative to an isotype control staining (grey).
From: Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, et al. (2017)
Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog 13(2): e1006206.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License. Published customer image:
Image caption:
ΔSRCR5 peripheral blood monocyte-derived macrophages (PMMs) are fully differentiated and express macrophage-specific markers.
Peripheral blood monocytes were isolated from the blood of the wild type (red), heterozygous (blue), and ΔSRCR5 (green) animals. Following cultivation in the presence of recombinant human CSF1 (rhCSF1) for seven days PMMs were analyzed by FACS. A) Co-staining with CD14-FITC and CD16-PE antibodies recognizing the native structure of the proteins (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). B) Co-staining with CD169-FITC and CD172a-PE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). C) Co-staining with SWC9 (CD203a)-FITC and CD151-RPE antibodies recognizing the native structure of the proteins (colored contour plots) relative to isotype controls (grey). D) Staining against the native structure of surface expressed CD163 (colored) relative to an isotype control staining (grey).
From: Burkard C, Lillico SG, Reid E, Jackson B, Mileham AJ, Ait-Ali T, et al. (2017)
Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog 13(2): e1006206.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
IMouse anti Porcine CD203a antibody, clone PM18-7 (MCA1973GA) used to evaluate CD203a expression on porcine pulmonary lesion cells by flow cytometry.
Image caption:
Representative dot-plots of CD14, CD172a, CD203a and SWC8 expression by CD163+ CD203a− and CD163+ CD203a+ cells from a lung lesion. (A) Gating of CD163+ and CD203a+ cells. (B) Light scatter properties of CD163+ CD203a− and CD163+ CD203a+ cells. (C) SLA-DR expression by CD163+ CD203a− and CD163+ CD203a+ cells (SLA-DR stained with PE-Cy5.5 had to be depicted against CD14 stained with SPRD due to strong spread of fluorescence from SPRD channel to PE-Cy5.5 channel). (D) CD172a, CD14 and SWC8 expression by CD163+ CD203a− and CD163+ CD203a+ cells.
From: Jarosova R, Ondrackova P, Leva L, Nedbalcova K, Vicenova M, Masek J, Volf J, Gebauer J, Do T, Guran R, Sladek Z, Dominguez J, Faldyna M. Cytokine expression by CD163+ monocytes in healthy and Actinobacillus pleuropneumoniae.-infected pigs.
Res Vet Sci. 2022 Jul 21;152:1-9.
doi: 10.1016/j.rvsc.2022.07.015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IF ResetMouse anti Pig CD203a
- Product Type
- Monoclonal Antibody
- Clone
- PM18-7
- Isotype
- IgG1
- Specificity
- CD203a
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD203a, clone PM18-7 recognizes porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the porcine homologue of human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007). Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of porcine alveolar macrophages. CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015). SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with P3X63-Ag8-653 murine myeloma cells (Kearney et al. 1979).
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD203a antibody
-
McCullough, K.C. et al. (1997) Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages.
Vet Immunol Immunopathol. 58 (3-4): 265-75. -
Dominguez, J. et al. (1998) Workshop studies with monoclonal antibodies identifying a novel porcine differentiation antigen, SWC9.
Vet Immunol Immunopathol. 60 (3-4): 343-9. -
Domínguez, J. et al. (1998) Porcine myelomonocytic markers: summary of the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60 (3-4): 329-41. -
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7. -
Basta, S. et al. (1999) Modulation of monocytic cell activity and virus susceptibility during differentiation into macrophages.
J Immunol. 162 (7): 3961-9. -
Basta, S. et al. (2001) Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility.
Immunology. 103 (4): 488-97. -
Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80 (1-2): 63-78. -
Cantu, E. et al. (2006) Depletion of pulmonary intravascular macrophages prevents hyperacute pulmonary xenograft dysfunction.
Transplantation. 81 (8): 1157-64.
View The Latest Product References
-
Petersen, C.B. et al. (2007) Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies.
Dev Comp Immunol. 31 (6): 618-31. -
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. -
Ondrackova, P. et al. (2013) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and< i> Salmonella-infected piglets by seven-colour flow cytometry.
Res Vet Sci. 94: 240 - 5. -
Tsai, Y.C. et al. (2014) Differences in the expression of innate immune response-modulating genes in blood monocytes between subclinically porcine circovirus type s (PCV2)-infected and PCV2-free pigs prior to and after lipopolysaccharide stimulation in vitro
Taiwan Vet J. 40 (01): 37-48. -
Hwang, J.H.et al. (2015) Characterization of monoclonal antibodies against porcine pulmonary alveolar macrophages of gnotobiotic miniature swine.
Biochem Biophys Res Commun. 461 (2): 427-34. -
Shao, L. et al. (2016) Tissue-specific mRNA expression profiles of porcine Toll-like receptors at different ages in germ-free and conventional pigs.
Vet Immunol Immunopathol. 171: 7-16. -
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206. -
Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
Dev Comp Immunol. 81: 95-104. -
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92. -
Zimmermann, C.E. et al. (2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.
J Stem Cells Regen Med. 17 (2): 49-55. -
Jarosova, R. et al. (2022) Cytokine expression by CD163+ monocytes in healthy and Actinobacillus pleuropneumoniae.-infected pigs.
Res Vet Sci. 152: 1-9.
Further Reading
-
Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54.
- Synonyms
- SWC9
MCA1973GA
154334
162948
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