CD169 antibody | 3B11/11
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|Mouse anti Pig CD169, clone 3B11/11 recognizes porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003) .
Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches.
Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) .
Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Porcine spleen
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
References for CD169 antibody
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109.
Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46.
Revilla, C. et al. (2009) Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 40 (3): 14.
Rodríguez-Gómez IM et al. (2015) PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate.
Vet Res. 46: 54.
Costa-Hurtado, M. et al. (2013) Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence.
Infect Immun. 81 (7): 2327-33.
Perdiguero, B. et al. (2008) Vaccinia virus A34 glycoprotein determines the protein composition of the extracellular virus envelope.
J Virol. 82 (5): 2150-60.
Perdiguero, B. & Blasco, R. (2006) Interaction between vaccinia virus extracellular virus envelope A33 and B5 glycoproteins.
J Virol. 80 (17): 8763-77.
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206.
View The Latest Product References
Whitworth, K.M. et al. (2016) Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus.
Nat Biotechnol. 34 (1): 20-2.
Wells, K.D. et al. (2017) Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 91 (2): pii: e01521-16.
Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
Dev Comp Immunol. 33 (3): 284-98.
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832.
Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
Int J Biol Sci. 15 (2): 481-492.
Li, P. et al. (2020) Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus.
J Vet Med Sci. 82 (9): 1404-9.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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