CD163 antibody | 2A10/11














Mouse anti Pig CD163:FITC
Mouse anti Pig CD163
Mouse anti Pig CD163:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2A10/11
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2311F | F | 0.1 mg |
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MCA2311GA | C F IF IP WB* | 0.1 mg |
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MCA2311PE | F | 100 Tests |
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Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q2VL90
- Entrez Gene
- CD163
- GO Terms
- GO:0005886 plasma membrane
- GO:0006953 acute-phase response
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
- Regulatory
- For research purposes only
Applications of CD163 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/50 | 1/200 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Western Blotting 1 | |||
Flow Cytometry | Neat | 1/10 |
- 1Clone 2A10/11 recognizes porcine CD163 under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Western Blotting
- Clone 2A10/11 detects a band of approximately 120kD in alveolar macrophage cell lysates under non-reducing conditions.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Application Based External Images
Flow Cytometry
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Product Specific References
Source Reference
-
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
Antibody Characterization Reference
-
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7.
References for CD163 antibody
-
Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis Sci. 43 (5): 1488-92. -
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. -
Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. -
Gómez del Moral M et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis.
J Virol. 73 (3): 2173-80. -
De Baere, M.I. et al. (2012) Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
Vet Res. 43: 47. -
Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46. -
Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163.
BMC Biotechnol. 10: 48. -
Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55. -
Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 41: 76. -
Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64. -
Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction.
Am J Physiol Heart Circ Physiol. 300: H693-701. -
Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163.
J Virol. 84: 1731-40. -
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. -
Costa-Hurtado, M. et al. (2013) Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence.
Infect Immun. 81 (7): 2327-33. -
Ma, H. et al. (2016) Crystal Structure of the Fifth Scavenger Receptor Cysteine-Rich Domain (SRCR5) from Porcine CD163 Reveals an Important Residue Involved in Porcine Reproductive and Respiratory Syndrome Virus Infection.
J Virol. pii: JVI.01897-16. [Epub ahead of print] -
Popescu, L. et al. (2017) Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1.
Virology. 501: 102-6. -
Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. -
Sang, Y. et al. (2014) Antiviral Regulation in Porcine Monocytic Cells at Different Activation States.
J Virol. pii: JVI.01714-14. -
Haslauer, C.M. et al. (2014) Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model.
J Inflamm (Lond). 11 (1): 34. -
Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244. -
Le Luduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22. -
Zhang, L. et al. (2016) Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection.
PLoS One. 11 (5): e0154238. -
Gu, M.J. et al. (2016) Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol.
Vet Res. 47: 25. -
Li, H. et al. (2015) Function of CD163 fragments in porcine reproductive and respiratory syndrome virus infection.
Int J Clin Exp Med. 8 (9): 15373-82. -
Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
Dev Comp Immunol. 65: 31-40. -
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. -
Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
J Diabetes Res. 2016: 3486727. -
Contreras, G.A. et al. (2016) Adipose tissue remodeling in late-lactation dairy cows during feed-restriction-induced negative energy balance.
J Dairy Sci. 99 (12): 10009-21. -
Garba, A. et al. (2017) Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells
PLOS ONE. 12 (10): e0186343. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
Li, L. et al. (2017) Generation of murine macrophage-derived cell lines expressing porcine CD163 that support porcine reproductive and respiratory syndrome virus infection.
BMC Biotechnol. 17 (1): 77. -
Wu, X. et al. (2018) Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line.
Biomed Res Int. 2018: 4315861. -
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206. -
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92. -
Bacou, E. et al. (2017) β2-adrenoreceptor stimulation dampens the LPS-induced M1 polarization in pig macrophages.
Dev Comp Immunol. 76: 169-76. -
Li, P. et al. (2020) Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus.
J Vet Med Sci. 82 (9): 1404-9.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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