CD163 antibody | 2A10/11















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- Product Type
- Monoclonal Antibody
- Clone
- 2A10/11
- Isotype
- IgG1
- Specificity
- CD163
Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
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Flow Cytometry | ![]() |
1/50 | 1/200 |
Immunofluorescence | ![]() |
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Immunohistology - Frozen | ![]() |
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Immunoprecipitation | ![]() |
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Western Blotting 1 | ![]() |
- 1Clone 2A10/11 recognizes porcine CD163 under non-reducing conditions.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
- Western Blotting
- Mouse anti Pig CD163 antibody, clone 2A10/11 detects a band of approximately 120 kDa in alveolar macrophage cell lysates under non-reducing conditions.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Description | Mouse IgG1 Negative Control |
Source Reference
-
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
Antibody Characterization Reference
-
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7. J Immunol. 162 (9): 5230-7.
References for CD163 antibody
-
Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis Sci. 43 (5): 1488-92. -
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. -
Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. -
Gómez del Moral M et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis.
J Virol. 73 (3): 2173-80. -
De Baere, M.I. et al. (2012) Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis.
Vet Res. 43: 47. -
Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46. -
Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163.
BMC Biotechnol. 10: 48. -
Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55.
View The Latest Product References
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Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.
Vet Res. 41: 76. -
Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64. -
Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction.
Am J Physiol Heart Circ Physiol. 300: H693-701. -
Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163.
J Virol. 84: 1731-40. -
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9. -
Costa-Hurtado, M. et al. (2013) Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence.
Infect Immun. 81 (7): 2327-33. -
Ma, H. et al. (2016) Crystal Structure of the Fifth Scavenger Receptor Cysteine-Rich Domain (SRCR5) from Porcine CD163 Reveals an Important Residue Involved in Porcine Reproductive and Respiratory Syndrome Virus Infection.
J Virol. pii: JVI.01897-16. [Epub ahead of print] -
Popescu, L. et al. (2017) Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1.
Virology. 501: 102-6. -
Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. -
Sang, Y. et al. (2014) Antiviral Regulation in Porcine Monocytic Cells at Different Activation States.
J Virol. pii: JVI.01714-14. -
Haslauer, C.M. et al. (2014) Gene expression of catabolic inflammatory cytokines peak before anabolic inflammatory cytokines after ACL injury in a preclinical model.
J Inflamm (Lond). 11 (1): 34. -
Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244. -
Le Luduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22. -
Zhang, L. et al. (2016) Developing a Triple Transgenic Cell Line for High-Efficiency Porcine Reproductive and Respiratory Syndrome Virus Infection.
PLoS One. 11 (5): e0154238. -
Gu, M.J. et al. (2016) Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol.
Vet Res. 47: 25. -
Li, H. et al. (2015) Function of CD163 fragments in porcine reproductive and respiratory syndrome virus infection.
Int J Clin Exp Med. 8 (9): 15373-82. -
Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
Dev Comp Immunol. 65: 31-40. -
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382-94. -
Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
J Diabetes Res. 2016: 3486727. -
Contreras, G.A. et al. (2016) Adipose tissue remodeling in late-lactation dairy cows during feed-restriction-induced negative energy balance.
J Dairy Sci. 99 (12): 10009-21. -
Garba, A. et al. (2017) Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells
PLOS ONE. 12 (10): e0186343. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
Li, L. et al. (2017) Generation of murine macrophage-derived cell lines expressing porcine CD163 that support porcine reproductive and respiratory syndrome virus infection.
BMC Biotechnol. 17 (1): 77. -
Wu, X. et al. (2018) Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line.
Biomed Res Int. 2018: 4315861. -
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206. -
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92. -
Bacou, E. et al. (2017) β2-adrenoreceptor stimulation dampens the LPS-induced M1 polarization in pig macrophages.
Dev Comp Immunol. 76: 169-76. -
Li, P. et al. (2020) Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus.
J Vet Med Sci. 82 (9): 1404-9. -
Pasternak, J.A. et al. (2019) Development and application of a porcine specific ELISA for the quantification of soluble CD163.
Vet Immunol Immunopathol. 210: 60-7. -
Franzoni, G. et al. (2022) Analyses of the Impact of Immunosuppressive Cytokines on Porcine Macrophage Responses and Susceptibility to Infection to African Swine Fever Viruses.
Pathogens. 11 (2): 166. -
Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
Vaccines. 10 (5): 684. -
Zhou, L. et al. (2022) Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model.
J Transl Med. 20 (1): 538. -
Štěpánová, H. et al. (2022) Characterization of Porcine Monocyte-Derived Macrophages Cultured in Serum-Reduced Medium.
Biology (Basel). 11(10):1457.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- UniProt
- Q2VL90
- Entrez Gene
- CD163
- GO Terms
- GO:0005886 plasma membrane
- GO:0006953 acute-phase response
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
MCA2311GA



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