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CD16 antibody | G7

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Anti Pig CD16 Antibody, clone G7 thumbnail image 1 Anti Pig CD16 Antibody, clone G7 thumbnail image 2 Anti Pig CD16 Antibody, clone G7 thumbnail image 3 Anti Pig CD16 Antibody, clone G7 thumbnail image 4 Anti Pig CD16 Antibody, clone G7 thumbnail image 5
Anti Pig CD16 Antibody, clone G7 gallery image 1
Figure A. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD16 (MCA1971F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD16 Antibody, clone G7 gallery image 2
Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse anti porcine CD16 (MCA1971PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD16 Antibody, clone G7 gallery image 3
Figure A. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse anti porcine CD45 (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD16 Antibody, clone G7 gallery image 4
Published customer image:
Mouse anti Porcine CD16 antibody, clone G7 (MCA1971GA used to identify CD16 expressing cells in porcine jejenum by immunofluorescence.
Image caption:
Colonisation affects mucosal MHCII+ APC subsets.
(A) Area of MHCII+ cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). (B) SIRPα staining in jejunal mucosa from a GF pig and (C) a colonised pig at 21 days of age. (B) (i) and (C) (i) show staining with antibodies to CD16 (red), SIRPα (green) and CD11R1 (blue). (B) (ii) and (C) (ii) show the same sections with the same cells in red and blue but with MHCII DR in green. In both (B) and (C), (iii) shows the red channel (SIRPα); (iv) shows the green channel (CD16), (v) shows the blue channel (CD11R1); (vi) shows the infra-red channel (MHC class II). Scale bar represents 10 μm. (D) Partitioning of MHCII between SIRPα+ (green shading) and SIRPα− (purple shading) APC subsets in the intestines of pigs at birth and from both treatment groups at 5 days and 21 days of age (see Table 1 for numbers of piglets in each experiment).

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012)
Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Pig CD16 Antibody, clone G7 gallery image 5
Published customer image:
FITC conjugated Mouse anti Pig CD16, clone G7 (MCA1971F) used to label porcine APCs by flow cytometry.
Image caption:
Influence of F18 fimbriae on antigen presenting capacity of intestinal antigen presenting cells. (A) Phenotypical analysis of small intestinal lamina propria mononuclear cells (LP MC). LP MCs were stained to determine CD172a, CD16 and MHCII expression in the live cell gate (CytoxBlue neg.) upon doublet discrimination. R1: lymphocytes; R6: MHCII+ activated T- and B-cells; R5: CD3+ T cells; R2-R4: myeloid cells. Representative plots for five separate experiments. (B) The LP MC population R6 consists mainly of IgM+ B-cells, while CD3+ T-cells make up the R5 population. (C) Morphological differences between CD16hi and CD16+ populations. (D) Macrophage-specific staining (CD68; red) of CD16hi and CD16+ population. Cell nuclei are shown in blue (Hoechst staining).

From: Van der Weken H, Sanz Garcia R, Sanders NN, Cox E, Devriendt B.
Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
Front Immunol. 2021 Oct 14;12:753371.
doi: 10.3389/fimmu.2021.753371.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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F IF

Mouse anti Pig CD16

Product Type
Monoclonal Antibody
Clone
G7
Isotype
IgG1
Specificity
CD16

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Product Code Applications Pack Size List Price Your Price Qty
MCA1971GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).

Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood leucocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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References for CD16 antibody

  1. Dato, M.E. et al. (1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
    Cell Immunol. 140 (2): 468-77.
  2. Wierda, W.G. et al. (1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.
    Cell Immunol. 146 (2): 270-83.
  3. Halloran, P.J. et al. (1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.
    Cell Immunol. 158 (2): 400-13.
  4. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
    Vet Immunol Immunopathol. 135 (3-4): 188-98.
  5. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
  6. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273 - 83.
  7. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
    PLoS Negl Trop Dis. 7: e2138.
  8. Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets.
    Clin Vaccine Immunol. 19 (9): 1499-508.
    9. View The Latest Product References
  9. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382 - 94.
  10. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
    Vet Res. 42: 9.
  11. Mussá, T. et al. (2011) Interaction of porcine conventional dendritic cells with swine influenza virus.
    Virology 420: 125-34.
  12. Vincent, I.E. et al. (2003) Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.
    J Virol. 77: 13288 - 300.
  13. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One 7: e33707.
  14. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162 (9): 5230-7.
  15. Lecours, M.P. et al. (2011) Characterization of porcine dendritic cell response to Streptococcus suis.
    Vet Res. 42: 72.
  16. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
    Clin Exp Immunol. 160 (3): 431-9.
  17. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
    Immunology 110: 440-9.
  18. Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
    Eur J Immunol. 42: 1261-71.
  19. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  20. Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
    J Immunol. Nov 11. pii: 1600672. [Epub ahead of print]
  21. Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.
  22. Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
    Vet Immunol Immunopathol. 178: 37-49.
  23. Waide, E.H. et al. (2015) Not All SCID Pigs Are Created Equally: Two Independent Mutations in the Artemis Gene Cause SCID in Pigs.
    J Immunol. 195 (7): 3171-9.
  24. Loss, H. et al. (2018) Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells.
    Vet Immunol Immunopathol. 203: 78-87.
  25. LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
    Vaccine. 34 (7): 914-22.
  26. Ferret-Bernard, S. et al. (2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.
    Nutrients. 12 (7): 2115.
  27. Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
    PLoS One. 14 (10): e0223647.
  28. Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
    Dev Comp Immunol. 81: 95-104.
  29. Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
  30. Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
    Injury. 52 (3): 426-33.
  31. Van der Weken, H. et al. (2021) Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
    Front Immunol. 12: 753371.
  32. Boettcher, A.N. et al. (2020) CD3ε+ Cells in Pigs With Severe Combined Immunodeficiency Due to Defects in ARTEMIS
    Frontiers in Immunology. 11 [Epub ahead of print].
  33. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.
  34. Štěpánová, H. et al. (2022) Characterization of Porcine Monocyte-Derived Macrophages Cultured in Serum-Reduced Medium.
    Biology (Basel). 11(10):1457.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
    Mol Immunol. 66 (1): 3-13.

Flow Cytometry

Immunofluorescence

Immunomagnetic assay

Synonyms
FcRIII
UniProt
Q28942
Entrez Gene
FCGR3B
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding

MCA1971GA

148178 157952 162371

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