CD11R3 antibody | 2F4/11
Mouse anti Porcine CD11R3 antibody, clone 2F4/11 (MCA2309) employed in the measurement of CD11R3 expression as a measure of neutrophil activation in a porcine model of metabolic syndrome by flow cytometry.
Image caption:
Systemic neutrophil activation can be evaluated by acute changes in CD11R3 expression and the percent circulating neutrophil population that is resistant to apoptosis in vitro. CD11R3 expression is increased acutely with neutrophil activation. With SCD exposure, CD11R3 expression decreased (a, n-6*, p=0.033). During the activated state neutrophil apoptosis is delayed. In these MetS animals, prior to SCD treatment, 34% of neutrophils are activated (resistant to apoptosis), while in healthy animals, less than 10% of circulating neutrophils are expected to be resistant to apoptosis. Analysis of apoptosis, before and after treatments, showed SCD Rx to move the neutrophil population to a less activated state, thus shifting toward naïve percentages of neutrophils resistant to apoptosis (b, n=9, p=0.023). For CD11R3 expression, only 2 of the 3 SCD Rx courses with three 6-hour treatments each were included in the analysis (n=6) due to an unanticipated analytic procedural complication.
From: Angela J. Westover, Kimberly A. Johnston, Deborah A. Buffington, and H. David Humes,
“An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome,”
Journal of Diabetes Research, vol. 2016, Article ID 3486727, 10 pages, 2016.
Mouse anti Pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family. Mouse anti Pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets (Dominguez et al. 2001).
Mouse anti Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages (Bullido et al. 1996).
Mouse anti Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages (Bullido et al. 1996).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine alveolar macrophages
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD11R3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to 1x106 cells in 100ul
- Histology Positive Control Tissue
- Porcine spleen
- Western Blotting
- Clone 2F4/11 detects a band of approximately 155 kDa in alveolar macrophage lysates under reducing conditions
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
Source Reference
-
Bullido R et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.
J Immunol Methods. 195 (1-2): 125-34.
References for CD11R3 antibody
-
Sbrana, S. et al. (2014) Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery Disease
J Cytol Histol 5:270. -
Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.
Vet Immunol Immunopathol. 80 (1-2): 111-9. -
Sánchez-Torres C et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. -
Van de Walle, G.R. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.
J Gen Virol. 84 (Pt 3): 629-37. -
Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
Xenotransplantation 7: 258-266 -
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162: 5230-7 -
Thorgersen, E.B. et al. (2010) Anti-inflammatory effects of C1-Inhibitor in porcine and human whole blood are independent of its protease inhibition activity.
Innate Immun. 16: 254-64 -
Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
FASEB J. 24: 712-22 -
Baert K et al. (2015) Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells.
Dev Comp Immunol. 48 (1): 192-203. -
Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9 -
Jacobsen, M.J. (2015) Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity
Journal of Diabetes Research Article ID 610546 [Epub ahead of print] -
Crisci, E. et al. (2012) Chimeric calicivirus-like particles elicit specific immune responses in pigs.
Vaccine. 30 (14): 2427-39. -
Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.
Eur J Dermatol. 23 (4): 456-66. -
Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
J Diabetes Res. 2016: 3486727. -
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54
