CD11R1 antibody | MIL4
Mouse anti Pig CD11R1 antibody, clone MIL4 (MCA1220GA) used for the identification of CD11R1 cell populations by flow cytometry.
Image caption:
Effect of Lactobacillus jensenii TL2937 on porcine intestinal epithelial (PIE) cells co-cultured with adherent population from swine Peyer's Patches (PPs) under inflammatory conditions. PIE-Adherent cells co-cultures were treated with L. jensenii TL2937 or L. plantarum TL2966 for 48 h. Untreated PIE-adherent cells co-cultures were used as controls. After lactobacilli stimulation, treated cells and untreated controls were challenge with ETEC (5 × 107 cells/ml). Expression of MHC-II, CD80/86, IL-10, and IFN-γ was studied in CD172a+CD11R1-, CD172a+CD11R1high, and CD172a-CD11R1low adherent cells by flow cytometric analysis. Values for bars with different letters were significantly different (P < 0.05). Values for bars with shared letters do not differ significantly. The results represent data from three independent experiments using ileal PPs from at least three different swine.
From: Suda Y, Villena J, Takahashi Y, Hosoya S, Tomosada Y, Tsukida K, Shimazu T, Aso H, Tohno M, Ishida M, Makino S, Ikegami S, Kitazawa H.
Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.
BMC Immunol. 2014 Jun 19;15:24.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD11R1, clone MIL4 (MCA1220GA) used to identify CD11R1 expressing cells by flow cytometry.
Image caption:
Phagocytosis of immunobiotic Lactobacillus jensenii TL2937 by porcine blood monocytes-derived dendritic cells (MoDCs). MoDCs were stained with antibodies for CD172a, and CD11R1 and two cell populations were defined: CD172a+CD11R1+ or CD172a−CD11R1+ (a). MoDCs were treated with the immunobiotic strain L. jensenii TL2937 or the non-immunoregulatory strain L. plantarum TL2766. Untreated MoDCs cells were used as controls. Bacterial phagocytosis by CD172a+CD11R1+ or CD172a−CD11R1+ MoDCs cells was evaluated using flow cytometric analysis (b). Histograms represent data from the flow cytometric analysis, as follows: cells treated with lactobacilli (solid line) and, untreated control cells (dot line). The results represent data from three independent experiments using different donors. In experiments cells were used in a concentration of 5.0 × 106 cells/well
From: Tsukida K, Takahashi T, Iida H, Kanmani P, Suda Y, Nochi T, Ohwada S, Aso H, Ohkawara S, Makino S, Kano H, Saito T, Villena J, Kitazawa H.
Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
BMC Immunol. 2016 Jun 24;17(1):21.
doi: 10.1186/s12865-016-0160-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD11R1, clone MIL4 (MCA1220GA) used to identify CD11R1 expressing cells by flow cytometry.
Image caption:
Phagocytosis of immunobiotic Lactobacillus jensenii TL2937 by mononuclear phagocytes from porcine Peyer’s patches (PPMPs). Adherent PPMPs were treated with the immunobiotic strain L. jensenii TL2937 or the non-immunoregulatory strain L. plantarum TL2766. Untreated adherent PPMPs were used as controls. Bacterial phagocytosis by adherent CD172a+CD11R1+ or CD172a−CD11R1+ PPMPs was evaluated using flow cytometric analysis. Histograms represent data from the flow cytometric analysis as follows: cells treated with lactobacilli (solid line), and untreated control cells (dot line) (a). Isolated CD172a+ PPMPs cells were obtained by a MACS cell separation system (magnetic cell labeling). Isolated CD172a+ cells were treated with L. jensenii TL2937 or L. plantarum TL2766. Bacterial phagocytosis was evaluated using flow cytometric analysis (b). In all the experiments cells were used in a concentration of 5.0 × 106 cells/well. Values of mean fluorescence intensity (MFI) are shown for each group. The results represent data from three independent experiments using different donors.
From: Tsukida K, Takahashi T, Iida H, Kanmani P, Suda Y, Nochi T, Ohwada S, Aso H, Ohkawara S, Makino S, Kano H, Saito T, Villena J, Kitazawa H.
Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
BMC Immunol. 2016 Jun 24;17(1):21.
doi: 10.1186/s12865-016-0160-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD11R1, clone MIL4 (MCA1220GA) used to identify CD11R1 expressing cells by immunofluorescence..
Image caption:
Generation of porcine blood monocytes-derived dendritic cells (MoDCs). Porcine immature MoDCs were generated from blood monocytes after the differentiation with GM-CSF and IL-4 for 5 days. Maturation of MoDCs was induced by stimulation with LPS. Cell morphology was evaluated by microscopic analysis (a). Expression of CD11R1, MHC-II, TLR2, and TLR4 in blood monocytes and mature MoDCs was determined by fluorescent microscopy (b). Photos represent data from three independent experiments using different donors. In experiments cells were used in a concentration of 5.0 × 106 cells/well. Scale bar = 50 μm.
From: Tsukida K, Takahashi T, Iida H, Kanmani P, Suda Y, Nochi T, Ohwada S, Aso H, Ohkawara S, Makino S, Kano H, Saito T, Villena J, Kitazawa H.
Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
BMC Immunol. 2016 Jun 24;17(1):21.
doi: 10.1186/s12865-016-0160-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Pig CD11R1
- Product Type
- Monoclonal Antibody
- Clone
- MIL4
- Isotype
- IgG1
- Specificity
- CD11R1
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD11R1, clone MIL4 recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Mouse anti Pig CD11R1, clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994). Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of ~95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001) Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine Lamina Propria Leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD11R1 antibody
-
Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45. -
Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.
Vet Immunol Immunopathol. 80 (1-2): 111-9. -
Ordway, D.et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
J Immunol. 179: 2532-41. -
Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88: 571-8. -
Cheng, Q. et al. (2010) Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets.
Int Immunopharmacol. 10: 611-8. -
Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55 (1): 124-31. -
Rank, R.G. et al. (2012) Effect of Inflammatory Response on In Vivo Competition between Two Chlamydial Variants in the Guinea Pig Model of Inclusion Conjunctivitis.
Infect Immun. 80: 612-9. -
Suda, Y. et al. (2014) Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.
BMC Immunol. 15: 24.
View The Latest Product References
-
Shegarfi, H. et al. (2015) Regulation of CCN1 (Cyr61) in a porcine model of intestinal ischemia/reperfusion.
Innate Immun. 21 (5): 453-62. -
Yeruva, L. et al. (2015) Chlamydial variants differ in ability to ascend the genital tract in the guinea pig model of chlamydial genital infection.
Infect Immun. 83 (8): 3176-83. -
Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
J Immunol. 197 (12): 4791-806. -
Tsukida, K. et al. (2016) Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells.
BMC Immunol. 17 (1): 21. -
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92. -
Iida, H. et al. (2019) Paraimmunobiotic Bifidobacteria Modulate the Expression Patterns of Peptidoglycan Recognition Proteins in Porcine Intestinal Epitheliocytes and Antigen Presenting Cells.
Cells. 8(8):891. -
Ferret-Bernard, S. et al. (2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.
Nutrients. 12 (7): 2115. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
MCA1220GA
155756
163787
1707
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Pig ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up