CD11R1 antibody | MIL4

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Anti Pig CD11R1 Antibody, clone MIL4 thumbnail image 1

Anti Pig CD11R1 Antibody, clone MIL4 thumbnail image 2

Anti Pig CD11R1 Antibody, clone MIL4 thumbnail image 3

Anti Pig CD11R1 Antibody, clone MIL4 thumbnail image 4

Anti Pig CD11R1 Antibody, clone MIL4 gallery image 1 — Pig peripheral blood lymphocytes stained with Mouse anti Pig CD11R1 (**MCA1220GA**) followed by Goat anti Mouse IgG:FITC (STAR117F).

Anti Pig CD11R1 Antibody, clone MIL4 gallery image 2 — Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD11R1 (**MCA1220GA**) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Anti Pig CD11R1 Antibody, clone MIL4 gallery image 3 — Figure A. Purified mouse anti porcine CD11R1 (**MCA1220GA**) detected with goat anti mouse IgG PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD11R1 (**MCA1220GA**) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD11a FITC (MCA2308F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Anti Pig CD11R1 Antibody, clone MIL4 gallery image 4 — **Published customer image:**
Mouse anti Pig CD11R1 antibody, clone MIL4 (**MCA1220GA**) used for the identification of CD11R1 cell populations by flow cytometry.
**Image caption:**
Effect of *Lactobacillus jensenii* TL2937 on porcine intestinal epithelial (PIE) cells co-cultured with adherent population from swine Peyer's Patches (PPs) under inflammatory conditions. PIE-Adherent cells co-cultures were treated with *L. jensenii* TL2937 or *L. plantarum* TL2966 for 48 h. Untreated PIE-adherent cells co-cultures were used as controls. After lactobacilli stimulation, treated cells and untreated controls were challenge with ETEC (5 × 10⁷ cells/ml). Expression of MHC-II, CD80/86, IL-10, and IFN-γ was studied in CD172a⁺CD11R1^-, CD172a⁺CD11R1^high, and CD172a^-CD11R1^low adherent cells by flow cytometric analysis. Values for bars with different letters were significantly different (P < 0.05). Values for bars with shared letters do not differ significantly. The results represent data from three independent experiments using ileal PPs from at least three different swine.

From: Suda Y, Villena J, Takahashi Y, Hosoya S, Tomosada Y, Tsukida K, Shimazu T, Aso H, Tohno M, Ishida M, Makino S, Ikegami S, Kitazawa H.
Immunobiotic *Lactobacillus jensenii* as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.
BMC Immunol. 2014 Jun 19;15:24.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Pig CD11R1

Product Type: Monoclonal Antibody
Clone: MIL4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1220GA	C F IP			0.1 mg

Mouse anti Pig CD11R1, clone MIL4 recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Mouse anti Pig CD11R1, clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994).

Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin β2 (CD18) of ~95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001)

Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980).

Product Details

Target Species

Pig

Species Cross-Reactivity

Target Species	Cross Reactivity
Human
Guinea Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Porcine Lamina Propria Leucocytes.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of CD11R1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/25	1/200
Immunohistology - Frozen
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for CD11R1 antibody

Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45.
Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.
Vet Immunol Immunopathol. 80 (1-2): 111-9.
Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88: 571-8.
Cheng, Q. et al. (2010) Administered CpG oligodeoxynucleotide induces mRNA expression of CXC and CC chemokines at the intestinal mucosa and PBMCs in piglets.
Int Immunopharmacol. 10: 611-8.
Ordway, D.et al. (2007) The cellular immune response to Mycobacterium tuberculosis infection in the guinea pig.
J Immunol. 179: 2532-41.
Shang, S. et al. (2010) The activity of TMC 207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
Antimicrob Agents Chemother. 55: 124-31.
Rank, R.G. et al. (2012) Effect of Inflammatory Response on In Vivo Competition between Two Chlamydial Variants in the Guinea Pig Model of Inclusion Conjunctivitis.
Infect Immun. 80: 612-9.
Suda, Y. et al. (2014) Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs.
BMC Immunol. 15: 24.
Shegarfi, H. et al. (2015) Regulation of CCN1 (Cyr61) in a porcine model of intestinal ischemia/reperfusion.
Innate Immun. 21 (5): 453-62.
Auray G et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
J Immunol. Nov 11. pii: 1600672. [Epub ahead of print]
Yeruva, L. et al. (2015) Chlamydial variants differ in ability to ascend the genital tract in the guinea pig model of chlamydial genital infection.
Infect Immun. 83 (8): 3176-83.
Sautter, C.A. et al. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.
Dev Comp Immunol. 84: 181-92.