CD79b antibody | AT107-2

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1		1/50	1/100
Immunoprecipitation
Western Blotting

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

References for CD79b antibody

1. Dornan, D. et al. (2009) Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma.
   Blood. 114: 2721-9.
2. Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
   J Immunol. 182: 1509-17
3. Poggi, A. et al (2017) Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.
   Vet Comp Oncol. 15 (2): 431-40.
4. Kreiss, A. et al. (2009) A histological and immunohistochemical analysis of lymphoid tissues of the Tasmanian devil.
   Anat Rec (Hoboken). 292: 611-20.
5. Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
   Vet Immunol Immunopathol. 147: 35-43.
6. Kandasamy S et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
   Vaccine. 32 (7): 816-24.
7. Whitney, J.L. et al. (2016) Immunohistochemical Analysis of Leucocyte Subsets in the Sinonasal Mucosa of Cats with Upper Respiratory Tract Aspergillosis.
   J Comp Pathol. 155 (2-3): 130-40.
8. McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
   J Zoo Wildl Med. 45 (4): 906-10.
View The Latest Product References
9. Allan, J.N. et al. (2019) CD79b Expression in Richter's Transformation
   Blood. 134 (Supplement_1): 4279.
10. Thalheim, L. et al. (2013) Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements.
    J Vet Intern Med. 27 (6): 1509-16.
11. Ouyang, K. et al. (2015) Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions.
    Vet Res. 46: 140.
12. Bennett, A.L> et al. (2017) Canine acute leukaemia: 50 cases (1989-2014).
    Vet Comp Oncol. 15 (3): 1101-14.
13. Borthwick, C.R. et al. (2019) An Examination of the Development and Localization of Key Immune Cells in Developing Pouch Young of the Red-Tailed Phascogale (Phascogale calura).
    Anat Rec (Hoboken). 302 (11): 1985-2002.
14. Tilly, H. et al. (2019) Polatuzumab vedotin in combination with immunochemotherapy in patients with previously untreated diffuse large B-cell lymphoma: an open-label, non-randomised, phase 1b–2 study
    The Lancet Oncology. 20 (7): 998-1010.
15. Sehn, L.H. et al. (2020) Polatuzumab Vedotin in Relapsed or Refractory Diffuse Large B-Cell Lymphoma.
    J Clin Oncol. 38 (2): 155-65.
16. Herrera, A.F. et al. (2022) Anti-CD79B Antibody-Drug Conjugate DCDS0780A in Patients with B-Cell Non-Hodgkin Lymphoma: Phase 1 Dose-Escalation Study.
    Clin Cancer Res. Jan 03 [Epub ahead of print].

Further Reading

1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
   Vet Res. 39: 54.

Synonyms

B29

RRID

AB_324357

UniProt

P15530

Entrez Gene

Cd79b

GO Terms

GO:0005515 protein binding

GO:0016021 integral to membrane

GO:0004888 transmembrane receptor activity

GO:0009897 external side of plasma membrane

GO:0019815 B cell receptor complex

GO:0050853 B cell receptor signaling pathway