CD79b antibody | AT107-2
Rat anti Mouse CD79b:FITC
Rat anti Mouse CD79b
- Product Type
- Monoclonal Antibody
- Clone
- AT107-2
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2209F | F* | 0.1 mg |
|
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| MCA2209FT | F* | 25 µg |
|
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| MCA2209T | E F* IP | 25 µg |
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| MCA2209 | E F* IP WB | 0.2 mg |
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Rat anti Mouse CD79b antibody, clone AT107-2 recognizes a cytoplasmic region of mouse B-cell antigen receptor complex-associated protein beta chain, also known as B-cell-specific glycoprotein B29, Ig-beta, or Immunoglobulin-associated B29 protein. CD79b is a 228 amino acid, including a 26 signal peptide ~40 kDa type I single pass transmembrane glycoprotein. CD79b is expressed by B lymphocytes and associates with CD79a to form a heterodimer, non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. Rat anti Mouse CD79b antibody, clone AT107-2 also recognizes a homologous region of human CD79b.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat Pig Dog Chicken Koala Tasmanian Devil Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Peptide containing 20 amino acid residues from mouse CD79b conjugated to Keyhole Limpet Hemocyanin (KLH).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised rat were fused with cells of the mouse NS-1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15530
- Entrez Gene
- Cd79b
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0050853 B cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of CD79b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | 1/5 | |
| ELISA | |||
| Flow Cytometry 1 | 1/50 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
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| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for CD79b antibody
-
Dornan, D. et al. (2009) Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma.
Blood. 114: 2721-9. -
Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK.
J Immunol. 182: 1509-17 -
Poggi, A. et al (2017) Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.
Vet Comp Oncol. 15 (2): 431-40. -
Kreiss, A. et al. (2009) A histological and immunohistochemical analysis of lymphoid tissues of the Tasmanian devil.
Anat Rec (Hoboken). 292: 611-20. -
Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
Vet Immunol Immunopathol. 147: 35-43. -
Kandasamy S et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
Vaccine. 32 (7): 816-24. -
Whitney, J.L. et al. (2016) Immunohistochemical Analysis of Leucocyte Subsets in the Sinonasal Mucosa of Cats with Upper Respiratory Tract Aspergillosis.
J Comp Pathol. 155 (2-3): 130-40. -
McCurdy, P. et al. (2014) Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).
J Zoo Wildl Med. 45 (4): 906-10. -
Allan, J.N. et al. (2019) CD79b Expression in Richter's Transformation
Blood. 134 (Supplement_1): 4279. -
Thalheim, L. et al. (2013) Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements.
J Vet Intern Med. 27 (6): 1509-16. -
Ouyang, K. et al. (2015) Evaluation of humoral immune status in porcine epidemic diarrhea virus (PEDV) infected sows under field conditions.
Vet Res. 46: 140. -
Bennett, A.L> et al. (2017) Canine acute leukaemia: 50 cases (1989-2014).
Vet Comp Oncol. 15 (3): 1101-14. -
Borthwick, C.R. et al. (2019) An Examination of the Development and Localization of Key Immune Cells in Developing Pouch Young of the Red-Tailed Phascogale (Phascogale calura).
Anat Rec (Hoboken). 302 (11): 1985-2002. -
Tilly, H. et al. (2019) Polatuzumab vedotin in combination with immunochemotherapy in patients with previously untreated diffuse large B-cell lymphoma: an open-label, non-randomised, phase 1b–2 study
The Lancet Oncology. 20 (7): 998-1010. -
Sehn, L.H. et al. (2020) Polatuzumab Vedotin in Relapsed or Refractory Diffuse Large B-Cell Lymphoma.
J Clin Oncol. 38 (2): 155-65.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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