CD13 antibody | R3-63
Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183) used for the identification of CD13 expressing cells in the blood brain barrier of normal and Pdfgb null mice by immunofluorescence.
Image caption:
Assessment of vessel pericyte coverage and blood-brain barrier integrity in aged Pdgfrbredeye/redeye and Pdgfb+/-; Pdgfrb+/- mice.
(ABC) Assessment of pericyte coverage. A CD13 and CD31 co-immunolabelling was performed on 50 μm-thick parasagittal vibratome sections. (A) Representative 2D projections of ~40 μm z-stacks taken from the dorsal pons of wild-type (left panels) and double heterozygous (right panels) animals; upper panels (red): CD13 immunostaining; lower panels: merged CD31 (green) and CD13 immunostainings. Inserts provide a more detailed indication of the coverage rate. Scale bar: 30 μm. The capillary surface coverage rate in Pdgfb+/-; Pdgfrb+/- (B) and Pdgfrbredeye/redeye mice (C) was calculated and is plotted as the percentage of the vessel surface enveloped by pericytes. Two pictures from the dorsal pons and two pictures from the cortex were analyzed for each animal. The standard deviation of pericyte coverage of 4 animals per genotype is indicated by the error bars. WT mice and heterozygous Pdgfrbredeye/redeye mice were used as controls. (DE) Blood-brain barrier permeability assessment in Pdgfb+/-; Pdgfrb+/- (D) and Pdgfrbredeye/redeye (E) mice. Lysine-fixable cadaverine conjugated to Alexa Fluor-555 was injected intravenously into the tail vein (5 mg/ml in saline) 2 hours before sacrifice. Fluorescence was measured in the brain homogenate and arbitrary fluorescence units (AFU) were normalized to the brain weight. Error bars indicate standard deviation of fluorescence level measurement in 4 different animals. (F). Pericyte coverage of vessels in calcification prone regions compared with non-calcification-prone regions. Different brain regions were assessed for pericyte coverage in Pdgfbret/ret mice and the result is plotted as the percentage of the vessel surface enveloped by pericytes. For each animal, two pictures from the dorsal pons (calcification-prone) and two pictures from the cortex (non-calcification-prone) were analyzed. The standard deviation of pericyte coverage of 4 animals per genotype is indicated by the error bars. *p<0,05 when comparing Pdgfbret/ret with Pdgfbret/+, #p<0,05 when comparing calcification-prone regions with non-calcification-prone regions in Pdgfbret/ret mice. (G). Analysis of blood-brain barrier integrity in calcification prone regions compared to non-calcification-prone regions. Alexa Fluor-555 conjugated cadaverine tracer was allowed to circulate for 2 hours prior to sacrifice of the mice. The calcification prone regions of the brain were microdissected, and after homogenizing of the tissue, fluorescence was measured and normalized over the tissue weight (AFU). The standard deviation of 4 animals per genotype is indicated by the error bars. *p<0,05 when comparing Pdgfbret/ret with Pdgfbret/+, #p<0,05 when comparing calcification-prone regions with non-calcification-prone regions in Pdgfbret/ret mice.
From: Citation: Vanlandewijck M, Lebouvier T, Andaloussi Mäe M, Nahar K, Hornemann S, Kenkel D, et al. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification.
PLoS ONE 10(11): e0143407.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Purified Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183GA) used for the detection of pericytes in murine brain by immunofluorescence on formalin fixed, agarose embedded vibratome sections.
Image caption:
AAV‐BR1‐mediated transduction of brain endothelial cells in vivoC57BL/6 mice (age 8 weeks) were injected with AAV‐BR1 harboring an eGFP reporter gene under the control of the CAG promoter. Images show representative examples of n = 6 mice.
A. Representative images from cerebellum, olfactory bulb, striatum, cerebral cortex, and the spinal cord, 14 days after vector injection. BR1‐eGFP‐transduced cells (green) were positive for the endothelial marker CD31 (red). Scale bars represent 250 μm.
B. Higher magnification confocal images. The endothelial marker CD31 (red) colocalizes with vector‐mediated eGFP expression (green). Scale bars represent 50 μm (upper panel) or 10 μm (lower panel).
C. CD13 staining of pericytes. The vector‐mediated eGFP expression pattern (green) does not colocalize with CD13 (red). Scale bars represent 250 μm (upper panel) or 10 μm (lower panel).
D. Aquaporin 4 staining of astrocytic endfeet. The vector‐mediated eGFP expression (green) does not colocalize with aquaporin 4 (red). Scale bars represent 100 μm (upper panel) or 10 μm (lower panel).
E. Expression of eGFP in primary brain endothelial cells prepared from C57BL/6 mice, 14 days after injection with AAV‐BR1‐eGFP. Scale bars represent 500 μm (upper panel) or 100 μm (lower panel).
From: Körbelin J, Dogbevia G, Michelfelder S, Ridder DA, Hunger A, Wenzel J, Seismann H, Lampe M, Bannach J, Pasparakis M, Kleinschmidt JA, Schwaninger M, Trepel M.
A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases.
EMBO Mol Med. 2016 Apr 22. pii: e201506078.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183) used to demonstrate pericytes in murine brain by immunofluorescence.
Image caption:
α-syn-GFP and pS129-α-syn inclusions are present in endothelial cells but not in pericytes.
Confocal images showing pS129-α-syn does not colocalizate with CD13+ pericytes in the striatum of TG mice at age of 3, 8 and 13 months. pS129-α-syn = phospho S129-alpha-synuclein. Scale bar: 10 μm.
From: Elabi O, Gaceb A, Carlsson R, Padel T, Soylu-Kucharz R, Cortijo I, Li W, Li JY, Paul G.
Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
Sci Rep. 2021 Jan 13;11(1):1120.
doi: 10.1038/s41598-020-80889-8..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183) used to demonstrate pericytes in murine brain by immunofluorescence.
Image caption:
Alterations in striatal vessel and pericyte density in TG mice. Confocal images showing CD13+ pericyte density in WT and TG mice at age of 3, 8 and 13 months.
Sci Rep. 2021 Jan 13;11(1):1120.
doi: 10.1038/s41598-020-80889-8..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183) used to reveal pericytes in murine brain by immunofluorescence.
Image caption:
Pericyte coverage analysis and quantification of brain capillary diameter. Pericyte coverage on brain capillaries and capillary diameter were quantified in 8- and 24-month-old mouse brain samples. (A) Representative 3D volume-rendered images of endothelial cells (red) and pericytes (green) in the cerebral cortex. The analysis was conducted using images obtained from the cerebral cortex (B) and the hippocampus (C). Bar graphs show the results of quantifications of pericyte coverage in each brain region. Three images were analyzed in each brain region per mouse. Data represent the mean ± SE, *P <0.05 and **P <0.01 according to a two-tailed unpaired t-test (n = 4–5 animals per group). Scale bar = 30 μm.
From: Kato T, Sekine Y, Nozaki H, Uemura M, Ando S, Hirokawa S, Onodera O.
Excessive Production of Transforming Growth Factor β1 Causes Mural Cell Depletion From Cerebral Small Vessels.
Front Aging Neurosci. 2020 Jun 3;12:151.
doi: 10.3389/fnagi.2020.00151.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD13 antibody, clone R3-63 (MCA2183) used to label pericytes in mouse brain by immunofluorescence.
Image caption:
Representative images of brain sections co-stained with AAV-X1 vector-mediated expression of eGFP (green), endothelial cell marker (podocalyxin, in cyan) and (in red, with yellow arrowheads) a marker for pericytes (CD13). N=3 mice were tested and examined.
From: Chen X, Wolfe DA, Bindu DS, et al.
Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates.
Nat Commun. 2023 Jun 8;14(1):3345.
doi: 10.1038/s41467-023-38582-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C IF ResetRat anti Mouse CD13
- Product Type
- Monoclonal Antibody
- Clone
- R3-63
- Isotype
- IgG2a
- Specificity
- CD13
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Hansen et al. 1993). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Mouse intestinal APN
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/200 | 1/1000 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100uμl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| Description | Rat IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for CD13 antibody
-
Kamoun, W.S. et al. (2009) Edema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in mice.
J Clin Oncol. 27: 2542-52. -
Hansen, A.S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules.
Eur J Immunol. 23 (9): 2358-64. -
Larsen, S.L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.
J Exp Med. 184 (1): 183-9. -
Rangel, R. et al. (2007) Impaired angiogenesis in aminopeptidase N-null mice.
Proc Natl Acad Sci U S A. 104: 4588-93. -
Lahdenranta, J. et al. (2007) Treatment of hypoxia-induced retinopathy with targeted proapoptotic peptidomimetic in a mouse model of disease.
FASEB J. 21: 3272-8. -
Li, P. et al. (2010) Use of adenoviral vectors to target chemotherapy to tumor vascular endothelial cells suppresses growth of breast cancer and melanoma.
Mol Ther. 18: 921-8. -
van Deventer, H.W. et al. (2008) C-C chemokine receptor 5 on pulmonary fibrocytes facilitates migration and promotes metastasis via matrix metalloproteinase 9.
Am J Pathol. 173: 253-64. -
Gabrilovac, J. et al. (2011) Expression, regulation and functional activities of aminopeptidase N (EC 3.4.11.2; APN; CD13) on murine macrophage J774 cell line.
Immunobiology. 216: 132-44.
View The Latest Product References
-
Ozawa, M.G. et al. (2008) Beyond receptor expression levels: the relevance of target accessibility in ligand-directed pharmacodelivery systems.
Trends Cardiovasc Med. 18: 126-32. -
Bertilaccio, M.T. et al. (2008) Vasculature-targeted tumor necrosis factor-alpha increases the therapeutic index of doxorubicin against prostate cancer.
Prostate. 68: 1105-15. -
Boström, M. et al. (2014) The hippocampal neurovascular niche during normal development and after irradiation to the juvenile mouse brain.
Int J Radiat Biol. 90: 778-89. -
Mayer-Barber, K.D. et al. (2011) Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection.
Immunity. 35: 1023-34. -
Winnicka, B. et al. (2010) CD13 is dispensable for normal hematopoiesis and myeloid cell functions in the mouse.
J Leukoc Biol. 88: 347-59. -
Ridder, D.A. et al. (2015) Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.
J Exp Med. 212 (10): 1529-49. -
Vanlandewijck, M. et al. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification.
PLoS One. 10 (11): e0143407. -
Körbelin J et al. (2016) A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases.
EMBO Mol Med. 8 (6): 609-25. -
Zotz, J.S.
et al. (2016) CD13/aminopeptidase N is a negative regulator of mast cell activation.
FASEB J. 30 (6): 2225-35. -
Sung, S.J. et al. (2017) Proximal Tubule CD73 Is Critical in Renal Ischemia-Reperfusion Injury Protection.
J Am Soc Nephrol. 28 (3): 888-902. -
Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
Proc Natl Acad Sci U S A. 114 (17): 4531-6. -
Elabi, O. et al. (2021) Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
Sci Rep. 11 (1): 1120. -
Kato, T. et al. (2020) Excessive Production of Transforming Growth Factor β1 Causes Mural Cell Depletion From Cerebral Small Vessels.
Front Aging Neurosci. 12: 151. -
Chen, X. et al. (2023) Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates.
Nat Commun. 14 (1): 3345.
- Synonyms
- Aminopeptidase N
- RRID
- AB_323691
- UniProt
- P97449
- Entrez Gene
- Anpep
- GO Terms
- GO:0001525 angiogenesis
- GO:0004177 aminopeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0016021 integral to membrane
- GO:0008270 zinc ion binding
- GO:0030154 cell differentiation
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