CD13 antibody | R3-63

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MCA2183
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C F IF P * 0.25 mg loader
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MCA2183GA
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C F IF P * 0.1 mg loader
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MCA2183T
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C F IF P * 25 µg loader
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Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150 kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.

Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Hansen et al. 1993).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Mouse intestinal APN
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the IR983F rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/1000
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

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Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0

References for CD13 antibody

  1. Kamoun, W.S. et al. (2009) Edema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in mice.
    J Clin Oncol. 27: 2542-52.
  2. Hansen, A.S. et al. (1993) A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules.
    Eur J Immunol. 23 (9): 2358-64.
  3. Larsen, S.L. et al. (1996) T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptides.
    J Exp Med. 184 (1): 183-9.
  4. Rangel, R. et al. (2007) Impaired angiogenesis in aminopeptidase N-null mice.
    Proc Natl Acad Sci U S A. 104: 4588-93.
  5. Lahdenranta, J. et al. (2007) Treatment of hypoxia-induced retinopathy with targeted proapoptotic peptidomimetic in a mouse model of disease.
    FASEB J. 21: 3272-8.
  6. Li, P. et al. (2010) Use of adenoviral vectors to target chemotherapy to tumor vascular endothelial cells suppresses growth of breast cancer and melanoma.
    Mol Ther. 18: 921-8.
  7. van Deventer, H.W. et al. (2008) C-C chemokine receptor 5 on pulmonary fibrocytes facilitates migration and promotes metastasis via matrix metalloproteinase 9.
    Am J Pathol. 173: 253-64.
  8. Gabrilovac, J. et al. (2011) Expression, regulation and functional activities of aminopeptidase N (EC 3.4.11.2; APN; CD13) on murine macrophage J774 cell line.
    Immunobiology. 216: 132-44.
  9. View The Latest Product References
  10. Ozawa, M.G. et al. (2008) Beyond receptor expression levels: the relevance of target accessibility in ligand-directed pharmacodelivery systems.
    Trends Cardiovasc Med. 18: 126-32.
  11. Bertilaccio, M.T. et al. (2008) Vasculature-targeted tumor necrosis factor-alpha increases the therapeutic index of doxorubicin against prostate cancer.
    Prostate. 68: 1105-15.
  12. Boström, M. et al. (2014) The hippocampal neurovascular niche during normal development and after irradiation to the juvenile mouse brain.
    Int J Radiat Biol. 90: 778-89.
  13. Mayer-Barber, K.D. et al. (2011) Innate and adaptive interferons suppress IL-1α and IL-1β production by distinct pulmonary myeloid subsets during Mycobacterium tuberculosis infection.
    Immunity. 35: 1023-34.
  14. Winnicka, B. et al. (2010) CD13 is dispensable for normal hematopoiesis and myeloid cell functions in the mouse.
    J Leukoc Biol. 88: 347-59.
  15. Ridder, D.A. et al. (2015) Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.
    J Exp Med. 212 (10): 1529-49.
  16. Vanlandewijck, M. et al. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification.
    PLoS One. 10 (11): e0143407.
  17. Körbelin J et al. (2016) A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases.
    EMBO Mol Med. 8 (6): 609-25.
  18. Zotz, J.S. et al. (2016) CD13/aminopeptidase N is a negative regulator of mast cell activation.
    FASEB J. 30 (6): 2225-35.
  19. Sung, S.J. et al. (2017) Proximal Tubule CD73 Is Critical in Renal Ischemia-Reperfusion Injury Protection.
    J Am Soc Nephrol. 28 (3): 888-902.
  20. Yanagida, K. et al. (2017) Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.
    Proc Natl Acad Sci U S A. 114 (17): 4531-6.
  21. Elabi, O. et al. (2021) Human α-synuclein overexpression in a mouse model of Parkinson's disease leads to vascular pathology, blood brain barrier leakage and pericyte activation.
    Sci Rep. 11 (1): 1120.
  22. Kato, T. et al. (2020) Excessive Production of Transforming Growth Factor β1 Causes Mural Cell Depletion From Cerebral Small Vessels.
    Front Aging Neurosci. 12: 151.

Immunofluorescence

Synonyms
Aminopeptidase N
RRID
AB_323691
UniProt
P97449
Entrez Gene
Anpep
GO Terms
GO:0001525 angiogenesis
GO:0004177 aminopeptidase activity
GO:0008237 metallopeptidase activity
GO:0016021 integral to membrane
GO:0008270 zinc ion binding
GO:0030154 cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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