Canine Heartworm antibody | HW1-39

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Mouse anti Canine Heartworm

Product Type
Monoclonal Antibody
Clone
HW1-39
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2554 E WB 0.25 mg
Mouse anti Canine Heartworm antibody, clone HW1-39 recognizes both male and female ether extracted parasite antigen of canine heartworm.

Canine heartworm infection, caused by the filarial nematode Dirofilaria immitis, is an insidious and potentially fatal condition transmitted by mosquitoes, which primarily affects the heart and lungs of dogs, but may also affect other species including cats, foxes, sea-lions, ferrets and humans.

Canine heartworm disease, resulting ultimately in damage to the pulmonary vasculature system, is largely attributed to the immunological response of the host to the presence of the adult parasites, and in many cases their microscopic offspring (microfilaria) which circulate in the bloodstream, but growing evidence suggests the involvement of further factors/mediators.

Product Details

Target Species
Invertebrates
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Canine Heartworm antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with product code MCA2555B as a detection reagent.
Western Blotting
MCA2554 detects band/s of approximately 170, 155, 98, 80, 70 and 62kDa under reducing conditions.

Product Specific References

Further Reading

  1. Mupanomunda, M. et al. (1997) Dirofilaria immitis: heartworm infection alters pulmonary artery endothelial cell behavior.
    J Appl Physiol (1985). 82 (2): 389-98.
  2. Uchide, T. & Saida, K. (2005) Elevated endothelin-1 expression in dogs with heartworm disease.
    J Vet Med Sci. 67 (11): 1155-61.