Infliximab antibody | AbD20436





Human anti Infliximab
Anti-infliximab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays for infliximab and biosimilars. It is suitable as the capture antibody in a PK bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD20436
- Isotype
- HuCAL Fab monovalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA234 | E | 0.1 mg |
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Human anti Infliximab, clone AbD20436 inhibits the binding of infliximab to TNFα and can be used as a capture antibody in a pharmacokinetic (PK) assay in the bridging format to measure free drug.
Infliximab (branded as Remicade®) is a chimeric IgG1 kappa monoclonal antibody approved for the treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab is directed against TNFα and acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-infliximab antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Infliximab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.12 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents.
This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Remicade® is a registered trademark of Janssen Biotech Inc. Inflectra® is a registered trademark of Hospira, a Pfizer Company. RENFLEXIS™ is a trademark of Merck Sharp & Dohme Corp.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Infliximab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA system or as a capture reagent in PK assay development together with HRP-conjugated HCA215 or HCA233 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Infliximab | HCA213 | E | 0.1 mg |
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Human anti Infliximab | HCA215 | E | 0.1 mg |
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Human anti Infliximab | HCA233 | E | 0.1 mg |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg |
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