Infliximab antibody | AbD20436

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Human anti Infliximab

Anti-infliximab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays for infliximab and biosimilars. It is suitable as the capture antibody in a PK bridging ELISA.

Product Type
Monoclonal Antibody
Clone
AbD20436
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA234 E 0.1 mg
Human anti Infliximab, clone AbD20436, is an affinity matured anti-idiotypic antibody in monovalent Fab format that specifically recognizes free infliximab but not infliximab bound to its target, Tumor Necrosis Factor Alpha (TNFα). It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples.

Human anti Infliximab, clone AbD20436 inhibits the binding of infliximab to TNFα and can be used as a capture antibody in a pharmacokinetic (PK) assay in the bridging format to measure free drug.

Infliximab (branded as Remicade®) is a chimeric IgG1 kappa monoclonal antibody approved for the treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab is directed against TNFα and acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-infliximab antibodies.

Product Details

Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Infliximab.
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.12 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

Remicade® is a registered trademark of Janssen Biotech Inc. Inflectra® is a registered trademark of Hospira, a Pfizer Company. RENFLEXIS™ is a trademark of Merck Sharp & Dohme Corp.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Infliximab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA system or as a capture reagent in PK assay development together with HRP-conjugated HCA215 or HCA233 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Rabbit anti DYKDDDDK Tag:HRP AHP1074P 0.1 mg E WB
Goat anti Histidine Tag:Biotin AHP1656B 0.1 mg E WB
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Rat anti DYKDDDDK Tag:HRP MCA4764P 0.1 mg E P WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Infliximab HCA213 0.1 mg E
Human anti Infliximab HCA215 0.1 mg E
Human anti Infliximab HCA233 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
Recombinant Human TNF Alpha PHP051 50 µg E FN WB