Histidine Tag antibody | AD1.1.10

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Mouse anti Histidine Tag:Alexa Fluor® 488

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:Alexa Fluor® 647

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:Alk.Phos.

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:Biotin

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:FITC

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:HRP

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:DyLight®549

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Mouse anti Histidine Tag:DyLight®800

Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and so detects proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in both mammalian and non-mammalian cell lines.

Product Type
Monoclonal Antibody
Clone
AD1.1.10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1396A488 F 100 Tests/1ml
MCA1396A647 F 1 ml
MCA1396A E WB 0.1 mg
MCA1396B E WB 0.1 mg
MCA1396F F IF 0.1 mg
MCA1396P E WB 0.1 mg
MCA1396GA E IP WB 0.1 mg
MCA1396 E IP WB 1 mg
MCA1396G E IP WB 2 mg
MCA1396D549GA F IF 0.1 mg
MCA1396D800GA WB 0.1 mg
Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.

In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Product Details

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®549 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
Pack Size: 0.1 mg, 2 mg
0.09%Sodium Azide
Pack Size: 1 mg
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
PAX6 transcription factor linked to histidine tag.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Histidine Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry
ELISA 1/20 1/1000
Western Blotting 1/1000 1/5000
ELISA 1/500 1/1000
Western Blotting 1/1000
Flow Cytometry 1/10
Immunofluorescence
ELISA 1/20 1/1000
Western Blotting 1/1000 1/5000
ELISA
Immunoprecipitation
Western Blotting 1/500 1/1000
Flow Cytometry
Immunofluorescence 1/50 1/200
Western Blotting 1/1000 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E
Proteus IMAC Mini Purification Kit PUR025 4 Units PP

Application Based External Images

ELISA

Flow Cytometry

Immunohistology - Frozen

Immunoprecipitation

Western Blotting

Product Specific References

References for Histidine Tag antibody

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    J Biol Chem. 276 (10): 7346-50.
  2. Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
    Immunol. 103: 301-309.
  3. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
    J Biol Chem. 282 (31): 22370-5.
  4. Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
    BMC Immunol. 8: 4.
  5. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
    Genes Brain Behav. 7 (2): 244-56.
  6. Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
    J Biol Chem. 284 (15): 9755-63.
  7. Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
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  8. Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
    PLoS One. 5: e10176.
  9. Bahi, A. et al. (2008) The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement.
    Neuropsychopharmacology. 33: 2726-34.
  10. Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
    BMC Biotechnol. 10: 8.
  11. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
    Mol Immunol. 45: 2570-80.
  12. De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.
    Microb Cell Fact. 11: 23.
  13. Saerens, D. et al. (2004) Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen.
    J Biol Chem. 279 (50): 51965-72.
  14. Than, N.G. et al. (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
    Placenta. 35: 855-65.
  15. Elders RC et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
    Vet Immunol Immunopathol. 159 (1-2): 29-40.
  16. Chin, S.E. et al. (2015) Isolation of high-affinity, neutralizing anti-idiotype antibodies by phage and ribosome display for application in immunogenicity and pharmacokinetic analyses.
    J Immunol Methods. 416: 49-58.
  17. Peyrassol, X. et al. (2016) Development by Genetic Immunization of Monovalent Antibodies (Nanobodies) Behaving as Antagonists of the Human ChemR23 Receptor.
    J Immunol. 196 (6): 2893-901.
  18. Kim H & Loparo JJ (2016) Multistep assembly of DNA condensation clusters by SMC.
    Nat Commun. 7: 10200.
  19. Borg M et al. (2014) A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration.
    J Cell Sci. 127 (Pt 22): 4927-39.
  20. De Meyer, T. et al. (2015) Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.
    Plant Biotechnol J. 13 (7): 938-47.
  21. Siddiqui AA et al. (2015) Humoral immune responses to a recombinant Plasmodium vivax tryptophan-rich antigen among Plasmodium vivax-infected patients and its localization in the parasite.
    Appl Biochem Biotechnol. 175 (4): 2166-77.
  22. Warnecke, A. et al. (2016) Nitration of MOG diminishes its encephalitogenicity depending on MHC haplotype.
    J Neuroimmunol. pii: S0165-5728(16)30422-2. [Epub ahead of print]
  23. Bertucci, A. et al. (2011) A new coral carbonic anhydrase in Stylophora pistillata.
    Mar Biotechnol (NY). 13 (5): 992-1002.
  24. Liu, C.C. et al. (2016) The Fimbrial Protein is a Virulence Factor and Potential Vaccine Antigen of
    Avibacterium paragallinarum
    .
    Avian Dis. 60 (3): 649-55.
  25. Boujon, C.L. et al. (2017) Development and validation of an immunohistochemistry procedure for the detection of a neurotropic bovine astrovirus.
    J Virol Methods. 239: 26-33.
  26. Cartwright, S.P. et al. (2017) Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris.
    BMC Res Notes. 10 (1): 340.
  27. Thanongsaksrikul, J. et al. (2018) Identification and production of mouse scFv to specific epitope of enterovirus-71 virion protein-2 (VP2).
    Arch Virol. Jan 22 [Epub ahead of print].
  28. Gunnarsen, K.S. et al. (2018) Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system.
    PLoS One. 13 (4): e0195868.