Histidine Tag antibody

Goat anti Histidine Tag:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histidine Tag

Product Code Applications Pack Size List Price Your Price Qty
AHP1656B
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E WB 0.1 mg loader
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AHP1656F
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IF 0.1 mg loader
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AHP1656
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SDS Safety Datasheet SDS
E * WB 0.1 mg loader
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Search for Batch Specific Datasheets

Goat anti-Histidine tag antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins.

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antiserum to Histidine Tag was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.