YKL-40 antibody | G06-10H11

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Mouse anti Human YKL-40

Product Type
Monoclonal Antibody
Clone
G06-10H11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6290GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human YKL-40 antibody, clone G06-10H11, recognizes YKL-40, also known as chitinase-3-like protein 1, 39kDA synovial protein and cartilage glycoprotein 39. YKL-40 is a glycoprotein and a member of the mammalian chitinase-like proteins (Johansen et al. 2006). It is expressed by a variety of cells including macrophages, neutrophils, chondrocytes, fibroblasts, vascular smooth muscle cells, endothelial cells and epithelials cells (Lee et al. 2011). YKL-40 induces the maturation of monocytes to macrophages and is secreted by macrophages during late stages of differentiation and by activated macrophages. It has also been shown to be an adhesion and migration factor for vascular cells (Rathcke & Vestergaard 2009).

The purified Mouse anti Human YKL-40 antibody, clone G06-10H11 (MCA6290GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human YKL-40 antibody, clone G03-2H1 (MCA6291B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
NS0 derived recombinant full length human YKL-40 (Tyr22-Thr383)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P36222
Entrez Gene
CHI3L1
GO Terms
GO:0004568 chitinase activity
GO:0005201 extracellular matrix structural constituent
GO:0005529 sugar binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006032 chitin catabolic process
GO:0043169 cation binding
Regulatory
For research purposes only

Applications of YKL-40 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human YKL-40:Biotin MCA6291B E 0.1 mg loader

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