WIPI2 antibody | 2A2

Mouse anti WIPI2

Product Type
Monoclonal Antibody
Clone
2A2
Isotype
IgG1
Specificity
WIPI2

Product Code Applications Pack Size List Price Your Price Qty
MCA5780GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF IP P WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human WIPI2 antibody, clone 2A2 recognies WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2), also known as WIPI49-like protein 2. WIPI2 is a 454 amino acid ~54 kDa autophagosomal marker containing three WD repeats. WIPI2 is a mammalian orthologue of the yeast protein Atg18 and is similarly recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes (Polson et al. 2010).

Human WIPI2 exists in multiple isoforms including WIPI2A, the canonical 454 amino acid isoform and WIPI2B with deletions towards both the N and C terminal regions. Mouse anti Human WIPI2 antibody, clone 2A2 was generated using a C-terminal sequence and recognizes both WIPI2A and WIPI2B by western blotting (Pantoom et al. 2020)

Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the C-terminus of WIPI2b (CSALRLDEDSEHPPMILRTD)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

References for WIPI2 antibody

  1. Polson, H.E. et al. (2010) Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
    Autophagy. 6 (4): 506-22.
  2. Dooley, H.C. et al. (2014) WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
    Mol Cell. 55 (2): 238-52.
  3. MacVicar, T.D. and Lane, J.D. (2014) Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation.
    J Cell Sci. 127: 2313-25.
  4. Karanasios, E. et al. (2014) Imaging autophagy.
    Curr Protoc Cytom. 69: 12.34.1-12.34.16.
  5. Gomez-Sanchez, J.A. et al. (2015) Schwann cell autophagy, myelinophagy, initiates myelin clearance from injured nerves.
    J Cell Biol. 210 (1): 153-68.
  6. Kjos, I. et al. (2017) Rab7b modulates autophagic flux by interacting with Atg4B.
    EMBO Rep. 18 (10): 1727-39.
  7. Nascimbeni, A.C. et al. (2017) ER-plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis.
    EMBO J. 36 (14): 2018-33.
  8. Pantoom, S. et al. (2020) RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein.
    Autophagy. : 1-15.
  9. View The Latest Product References
  10. Deitersen, J. et al. (2021) High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol.
    Cell Death Dis. 12 (6): 560.
  11. Boukhalfa, A. et al. (2020) PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress.
    Nat Commun. 11 (1): 294.

Immunofluorescence

Western Blotting

RRID
AB_10845951
UniProt
Q9Y4P8
Q80W47
Entrez Gene
Wipi2
WIPI2
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005829 cytosol
GO:0032266 phosphatidylinositol-3-phosphate binding
GO:0034045 pre-autophagosomal structure membrane
GO:0080025 phosphatidylinositol-3,5-bisphosphate binding
GO:0043234 protein complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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