WIPI2 antibody | 2A2

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Mouse anti WIPI2

Product Type
Monoclonal Antibody
Clone
2A2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5780GA
Datasheet
IF IP P WB
SDS
0.1 mg loader

Mouse anti Human WIPI2 antibody, clone 2A2 recognies WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2), also known as WIPI49-like protein 2. WIPI2 is a 454 amino acid ~54 kDa autophagosomal marker containing three WD repeats. WIPI2 is a mammalian orthologue of the yeast protein Atg18 and is similarly recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes (Polson et al. 2010).

Human WIPI2 exists in multiple isoforms including WIPI2A, the canonical 454 amino acid isoform and WIPI2B with deletions towards both the N and C terminal regions. Mouse anti Human WIPI2 antibody, clone 2A2 was generated using a C-terminal sequence and recognizes both WIPI2A and WIPI2B by western blotting (Pantoom et al. 2020)

Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to the C-terminus of WIPI2b (CSALRLDEDSEHPPMILRTD)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y4P8
Q80W47
Entrez Gene
Wipi2
WIPI2
GO Terms
GO:0000045 autophagic vacuole assembly
GO:0005829 cytosol
GO:0032266 phosphatidylinositol-3-phosphate binding
GO:0034045 pre-autophagosomal structure membrane
GO:0080025 phosphatidylinositol-3,5-bisphosphate binding
GO:0043234 protein complex
Regulatory
For research purposes only

Applications of WIPI2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for WIPI2 antibody

  1. Polson, H.E. et al. (2010) Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
    Autophagy. 6 (4): 506-22.
  2. Dooley, H.C. et al. (2014) WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
    Mol Cell. 55 (2): 238-52.
  3. MacVicar, T.D. and Lane, J.D. (2014) Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation.
    J Cell Sci. 127: 2313-25.
  4. Karanasios, E. et al. (2014) Imaging autophagy.
    Curr Protoc Cytom. 69: 12.34.1-12.34.16.
  5. Gomez-Sanchez, J.A. et al. (2015) Schwann cell autophagy, myelinophagy, initiates myelin clearance from injured nerves.
    J Cell Biol. 210 (1): 153-68.
  6. Kjos, I. et al. (2017) Rab7b modulates autophagic flux by interacting with Atg4B.
    EMBO Rep. 18 (10): 1727-39.
  7. Nascimbeni, A.C. et al. (2017) ER-plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis.
    EMBO J. 36 (14): 2018-33.
  8. Pantoom, S. et al. (2020) RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein.
    Autophagy. : 1-15.
  9. Deitersen, J. et al. (2021) High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol.
    Cell Death Dis. 12 (6): 560.

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