WIPI2 antibody | 2A2
Mouse anti Human WIPI2 antibody, clone 2A2 (MCA5780) used to evaluate expression of the shortened isoform of human WIPI2, WIPI2B by western blotting.
Image caption:
Recombinant wild-type or mutant GST-RAB33B were incubated with cell lysate from HeLa cells previously incubated in EBSS for 2 h as indicated. Samples were subjected to affinity isolation using glutathione beads and immunoblot analysis. WIPI2B binding was trapped by crosslinking.
I: Input, FT: Flow-through, W: Wash, E: Elution.
From: Pantoom S, Konstantinidis G, Voss S, Han H, Hofnagel O, Li Z, Wu YW.
RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein.
Autophagy. 2020 Sep 22:1-15.
doi: 10.1080/15548627.2020.1822629.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human WIPI2 antibody, clone 2A2 recognies WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2), also known as WIPI49-like protein 2. WIPI2 is a 454 amino acid ~54 kDa autophagosomal marker containing three WD repeats. WIPI2 is a mammalian orthologue of the yeast protein Atg18 and is similarly recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes (Polson et al. 2010).
Human WIPI2 exists in multiple isoforms including WIPI2A, the canonical 454 amino acid isoform and WIPI2B with deletions towards both the N and C terminal regions. Mouse anti Human WIPI2 antibody, clone 2A2 was generated using a C-terminal sequence and recognizes both WIPI2A and WIPI2B by western blotting (Pantoom et al. 2020)
Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014).
Human WIPI2 exists in multiple isoforms including WIPI2A, the canonical 454 amino acid isoform and WIPI2B with deletions towards both the N and C terminal regions. Mouse anti Human WIPI2 antibody, clone 2A2 was generated using a C-terminal sequence and recognizes both WIPI2A and WIPI2B by western blotting (Pantoom et al. 2020)
Mouse anti Human WIPI2 antibody, clone 2A2 has been used for the immunofluorescent detection of WIPI2 in the human retinal epithelial cell line RPE1 (MacVicar and Lane 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Synthetic peptide corresponding to the C-terminus of WIPI2b (CSALRLDEDSEHPPMILRTD)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y4P8
- Q80W47
- Entrez Gene
- Wipi2
- WIPI2
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0005829 cytosol
- GO:0032266 phosphatidylinositol-3-phosphate binding
- GO:0034045 pre-autophagosomal structure membrane
- GO:0080025 phosphatidylinositol-3,5-bisphosphate binding
- GO:0043234 protein complex
- Regulatory
- For research purposes only
Applications of WIPI2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for WIPI2 antibody
-
Polson, H.E. et al. (2010) Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Autophagy. 6 (4): 506-22. -
Dooley, H.C. et al. (2014) WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
Mol Cell. 55 (2): 238-52. -
MacVicar, T.D. and Lane, J.D. (2014) Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation.
J Cell Sci. 127: 2313-25. -
Karanasios, E. et al. (2014) Imaging autophagy.
Curr Protoc Cytom. 69: 12.34.1-12.34.16. -
Gomez-Sanchez, J.A. et al. (2015) Schwann cell autophagy, myelinophagy, initiates myelin clearance from injured nerves.
J Cell Biol. 210 (1): 153-68. -
Kjos, I. et al. (2017) Rab7b modulates autophagic flux by interacting with Atg4B.
EMBO Rep. 18 (10): 1727-39. -
Nascimbeni, A.C. et al. (2017) ER-plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis.
EMBO J. 36 (14): 2018-33. -
Pantoom, S. et al. (2020) RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein.
Autophagy. : 1-15.
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