von Willebrand Factor antibody | RFF-VIII R/1

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Mouse anti Human von Willebrand Factor

Product Type
Monoclonal Antibody
Clone
RFF-VIII R/1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4682
Datasheet
C E PP R
SDS
0.5 mg loader

MCA4682G
Datasheet
C E PP R
SDS
0.2 mg loader

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P04275
Entrez Gene
VWF
GO Terms
GO:0001948 glycoprotein binding
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0005783 endoplasmic reticulum
GO:0005178 integrin binding
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0007597 blood coagulation, intrinsic pathway
GO:0019865 immunoglobulin binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031589 cell-substrate adhesion
GO:0033093 Weibel-Palade body
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0047485 protein N-terminus binding
Regulatory
For research purposes only

Applications of von Willebrand Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Protein Purification
Radioimmunoassays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil, thymus, liver, spleen or kidney.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for von Willebrand Factor antibody

  1. Goodall, A.H. et al. (1985) An immunoradiometric assay for human factor VIII/von Willebrand factor (VIII:vWF) using a monoclonal antibody that defines a functional epitope.
    Br J Haematol. 59 (4): 565-77.
  2. Goodall, A.H. & Meyer, D. (1985) Registry of monoclonal antibodies to factor VIII and von Willebrand factor. International Committee on Thrombosis and Haemostasis.
    Thromb Haemost. 54 (4): 878-91.
  3. Chand, S. et al. (1986) A two-site, monoclonal antibody-based immunoassay for von Willebrand factor--demonstration that vWF function resides in a conformational epitope.
    Thromb Haemost. 55 (3): 318-24.
  4. Kraus, E. et al. (2014) Platelet-free shear flow assay facilitates analysis of shear-dependent functions of VWF and ADAMTS13.
    Thromb Res. 2134: 1285-91.
  5. Chen, Y.J. et al. (2015) Blood-brain barrier KCa3.1 channels: evidence for a role in brain Na uptake and edema in ischemic stroke.
    Stroke. 46 (1): 237-44.
  6. Kölm, R. et al. (2016) Von Willebrand Factor Interacts with Surface-Bound C1q and Induces Platelet Rolling.
    J Immunol. Oct 3. pii: 1501876. [Epub ahead of print]

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