Vitronectin antibody | 8E6 (1.110)
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Mouse anti Human vitronectin antibody recognizes human vitronectin and will react with the native and denatured forms. It does not cross react with fibronectin.Vitronectin is one of the 2 most important cell adhesive proteins found in the blood and extracellular matrix.
It exists as a mixture of 2 monomeric glycoproteins that mediate cell adhesion through an interaction between the R-G-D sequence, present in the cell binding domains, and several different integrin receptors. It is also known as a complement lysis inhibitor and as a scavenger protein for macromolecular products of the complement and homeostasis cascades.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Purified vitronectin from human plasma.
- Approx. Protein Concentrations
- 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04004
- Entrez Gene
- VTN
- GO Terms
- GO:0005044 scavenger receptor activity
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008201 heparin binding
- GO:0010951 negative regulation of endopeptidase activity
- GO:0014911 positive regulation of smooth muscle cell migration
- GO:0030195 negative regulation of blood coagulation
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0032092 positive regulation of protein binding
- GO:0033627 cell adhesion mediated by integrin
- GO:0048260 positive regulation of receptor-mediated endocytosis
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0061302 smooth muscle cell-matrix adhesion
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- GO:0090303 positive regulation of wound healing
- Regulatory
- For research purposes only
Applications of Vitronectin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5 µg/ml | 20 µg/ml | |
| Immunohistology - Frozen | 5 µg/ml | 20 µg/ml | |
| Western Blotting | 5 µg/ml | 20 µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Source Reference
-
Hayman, E.G. et al. (1983) Serum spreading factor (vitronectin) is present at the cell surface and in tissues.
Proc Natl Acad Sci U S A. 80 (13): 4003-7.
References for Vitronectin antibody
-
Gladson, C.L. & Cheresh, D.A. (1991) Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells.
J Clin Invest. 88 (6): 1924-32. -
Kost, C. et al. (1996) Limited plasmin proteolysis of vitronectin. Characterization of the adhesion protein as morpho-regulatory and angiostatin-binding factor.
Eur J Biochem. 236 (2): 682-8. -
Kost, C. et al. (1992) Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation.
J Biol Chem. 267 (17): 12098-105. -
Dahm, L.M. & Bowers, C.W. (1998) Vitronectin regulates smooth muscle contractility via alphav and beta1 integrin.
J Cell Sci. 111 ( Pt 9): 1175-83. -
Aaboe, M. et al. (2003) Vitronectin in human breast carcinomas.
Biochim Biophys Acta. 1638 (1): 72-82.
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