Vitronectin antibody | 8E6 (1.110)
Insert meta-description and applications
Image caption:
Limited proteolysis of purified vitronectin by plasmin. Human vitronectin was exposed to plasmin at different time intervals (0, 2, 5, 10, 20, 40,80, 120 min) and parallel samples (lanes 1-8) were subjected to SDS-polyacrylamide gel electrophoresis and subsequent Western blot analysis using monoclonal antibodies P1C5 or 8E6. Note the disappearance of the intact vitronectin band (rec- ognized by PlC5) with concomitant appearance of an intermediate cleavage product of M, = 61,000 (arrowhead), recognized by mono- clonal antibody 8E6. Further proteolytic processing of vitronectin by plasmin also destroys the 8E6 epitope.
From: Kost C, Stüber W, Ehrlich HJ, Pannekoek H, Preissner KT.
Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation.
J Biol Chem. 1992 Jun 15;267(17):12098-105.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Vitronectin
- Product Type
- Monoclonal Antibody
- Clone
- 8E6 (1.110)
- Isotype
- IgG1
- Specificity
- Vitronectin
Antibody Quality and Performance Guarantee
| Mouse anti Human vitronectin antibody, clone 8E6 recognizes human vitronectin and will react with the native and denatured forms. Mouse anti Human vitronectin antibody, clone 8E6 does not cross react with fibronectin. Vitronectin is an important cell adhesive protein, found in the blood and extracellular matrix. It exists as a mixture of two monomeric glycoproteins that mediate cell adhesion through an interaction between the R-G-D motif, present in the cell binding domains, and several different integrin receptors. Vitronectin is also known as a complement lysis inhibitor (Milis et al. 1993) and as a scavenger protein for macromolecular products of the complement and homeostasis cascades (Stanley et al. 1986). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified vitronectin from human plasma.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5 µg/ml | 20 µg/ml |
| Immunohistology - Frozen | |
5 µg/ml | 20 µg/ml |
| Western Blotting | |
5 µg/ml | 20 µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Source Reference
-
Hayman, E.G. et al. (1983) Serum spreading factor (vitronectin) is present at the cell surface and in tissues.
Proc Natl Acad Sci U S A. 80 (13): 4003-7.
References for Vitronectin antibody
-
Gladson, C.L. & Cheresh, D.A. (1991) Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells.
J Clin Invest. 88 (6): 1924-32. -
Kost, C. et al. (1992) Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation.
J Biol Chem. 267 (17): 12098-105. -
Kost, C. et al. (1996) Limited plasmin proteolysis of vitronectin. Characterization of the adhesion protein as morpho-regulatory and angiostatin-binding factor.
Eur J Biochem. 236 (2): 682-8. -
Dahm, L.M. & Bowers, C.W. (1998) Vitronectin regulates smooth muscle contractility via alphav and beta1 integrin.
J Cell Sci. 111 ( Pt 9): 1175-83. -
Aaboe, M. et al. (2003) Vitronectin in human breast carcinomas.
Biochim Biophys Acta. 1638 (1): 72-82.
- RRID
- AB_2216300
- UniProt
- P04004
- Entrez Gene
- VTN
- GO Terms
- GO:0005044 scavenger receptor activity
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008201 heparin binding
- GO:0010951 negative regulation of endopeptidase activity
- GO:0014911 positive regulation of smooth muscle cell migration
- GO:0030195 negative regulation of blood coagulation
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- View More GO Terms
- GO:0032092 positive regulation of protein binding
- GO:0033627 cell adhesion mediated by integrin
- GO:0048260 positive regulation of receptor-mediated endocytosis
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0061302 smooth muscle cell-matrix adhesion
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- GO:0090303 positive regulation of wound healing
9590-1222
164751
300615
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up