Vitronectin antibody | 8E6 (1.110)

Mouse anti Human Vitronectin

Product Type
Monoclonal Antibody
Clone
8E6 (1.110)
Isotype
IgG1
Specificity
Vitronectin

Product Code Applications Pack Size List Price Your Price Qty
9590-1222
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SDS Safety Datasheet SDS
C E WB 25 µl loader
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loader
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Mouse anti Human vitronectin antibody, clone 8E6 recognizes human vitronectin and will react with the native and denatured forms. Mouse anti Human vitronectin antibody, clone 8E6 does not cross react with fibronectin.

Vitronectin is an important cell adhesive protein, found in the blood and extracellular matrix. It exists as a mixture of two monomeric glycoproteins that mediate cell adhesion through an interaction between the R-G-D motif, present in the cell binding domains, and several different integrin receptors. Vitronectin is also known as a complement lysis inhibitor (Milis et al. 1993) and as a scavenger protein for macromolecular products of the complement and homeostasis cascades (Stanley et al. 1986).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified vitronectin from human plasma.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5 µg/ml 20 µg/ml
Immunohistology - Frozen 5 µg/ml 20 µg/ml
Western Blotting 5 µg/ml 20 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Source Reference

  1. Hayman, E.G. et al. (1983) Serum spreading factor (vitronectin) is present at the cell surface and in tissues.
    Proc Natl Acad Sci U S A. 80 (13): 4003-7.

References for Vitronectin antibody

  1. Gladson, C.L. & Cheresh, D.A. (1991) Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells.
    J Clin Invest. 88 (6): 1924-32.
  2. Kost, C. et al. (1996) Limited plasmin proteolysis of vitronectin. Characterization of the adhesion protein as morpho-regulatory and angiostatin-binding factor.
    Eur J Biochem. 236 (2): 682-8.
  3. Kost, C. et al. (1992) Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation.
    J Biol Chem. 267 (17): 12098-105.
  4. Dahm, L.M. & Bowers, C.W. (1998) Vitronectin regulates smooth muscle contractility via alphav and beta1 integrin.
    J Cell Sci. 111 ( Pt 9): 1175-83.
  5. Aaboe, M. et al. (2003) Vitronectin in human breast carcinomas.
    Biochim Biophys Acta. 1638 (1): 72-82.

Immunohistology - Frozen

RRID
AB_2216300
UniProt
P04004
Entrez Gene
VTN
GO Terms
GO:0005044 scavenger receptor activity
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008201 heparin binding
GO:0010951 negative regulation of endopeptidase activity
GO:0014911 positive regulation of smooth muscle cell migration
GO:0030195 negative regulation of blood coagulation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0032092 positive regulation of protein binding
GO:0033627 cell adhesion mediated by integrin
GO:0048260 positive regulation of receptor-mediated endocytosis
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0061302 smooth muscle cell-matrix adhesion
GO:0071062 alphav-beta3 integrin-vitronectin complex
GO:0090303 positive regulation of wound healing
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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