Vitronectin antibody | 8E6 (1.110)

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Mouse anti Human Vitronectin

Product Type
Monoclonal Antibody
Clone
8E6 (1.110)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9590-1222 C E WB 25 µl
Mouse anti Human vitronectin antibody recognizes human vitronectin and will react with the native and denatured forms. It does not cross react with fibronectin.

Vitronectin is one of the 2 most important cell adhesive proteins found in the blood and extracellular matrix. It exists as a mixture of 2 monomeric glycoproteins that mediate cell adhesion through an interaction between the R-G-D sequence, present in the cell binding domains, and several different integrin receptors. It is also known as a complement lysis inhibitor and as a scavenger protein for macromolecular products of the complement and homeostasis cascades.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified vitronectin from human plasma.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04004 Related reagents
Entrez Gene
VTN Related reagents
GO Terms
GO:0005044 scavenger receptor activity
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008201 heparin binding
GO:0010951 negative regulation of endopeptidase activity
GO:0014911 positive regulation of smooth muscle cell migration
GO:0030195 negative regulation of blood coagulation
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0032092 positive regulation of protein binding
GO:0033627 cell adhesion mediated by integrin
GO:0048260 positive regulation of receptor-mediated endocytosis
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0061302 smooth muscle cell-matrix adhesion
GO:0071062 alphav-beta3 integrin-vitronectin complex
GO:0090303 positive regulation of wound healing
Regulatory
For research purposes only

Applications of Vitronectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5 µg/ml 20 µg/ml
Immunohistology - Frozen 5 µg/ml 20 µg/ml
Western Blotting 5 µg/ml 20 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Frozen

Product Specific References

Source Reference

  1. Hayman, E.G. et al. (1983) Serum spreading factor (vitronectin) is present at the cell surface and in tissues.
    Proc Natl Acad Sci U S A. 80 (13): 4003-7.

References for Vitronectin antibody

  1. Gladson, C.L. & Cheresh, D.A. (1991) Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells.
    J Clin Invest. 88 (6): 1924-32.
  2. Kost, C. et al. (1996) Limited plasmin proteolysis of vitronectin. Characterization of the adhesion protein as morpho-regulatory and angiostatin-binding factor.
    Eur J Biochem. 236 (2): 682-8.
  3. Kost, C. et al. (1992) Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation.
    J Biol Chem. 267 (17): 12098-105.
  4. Dahm, L.M. & Bowers, C.W. (1998) Vitronectin regulates smooth muscle contractility via alphav and beta1 integrin.
    J Cell Sci. 111 ( Pt 9): 1175-83.
  5. Aaboe, M. et al. (2003) Vitronectin in human breast carcinomas.
    Biochim Biophys Acta. 1638 (1): 72-82.