UBE3A antibody | 2F6
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P IF WB ResetMouse anti Human UBE3A:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2F6
- Isotype
- IgG2a
- Specificity
- UBE3A
Antibody Quality and Performance Guarantee
| Mouse anti Human UBE3A antibody, clone 2F6 recognizes human Ubiquitin-protein ligase E3A, also known as UBE3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP or Renal carcinoma antigen NY-REN-54. UBE3A is an 875 amino acid ~95 kDa E3 ubiquitin-protein ligase. Mutations of the UBE3A gene can lead to the development of Angelman syndrome (AS) a neurodevelopmental disorder characterized by by severe motor and intellectual retardation, hypotonia, ataxia and jerky limb movements (Sadikovic et al. 2014). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 51-151 of human UBE3A
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Immunohistology - Paraffin 1 | |
0.1 | 10 ug/ml |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for UBE3A antibody
-
Wolyniec, K. et al. (2012) E6AP ubiquitin ligase regulates PML-induced senescence in Myc-driven lymphomagenesis.
Blood. 120: 822-32. -
Paul, P.J. et al. (2016) Restoration of tumor suppression in prostate cancer by targeting the E3 ligase E6AP.
Oncogene. 35 (48): 6235-45.
- Synonyms
- E6AP
- RRID
- AB_2211795
- UniProt
- Q05086
- Entrez Gene
- UBE3A
MCA3532Z
144539
150755
161654
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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