UBE3A antibody | 2F6



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P IF WB ResetMouse anti Human UBE3A:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2F6
- Isotype
- IgG2a
- Specificity
- UBE3A
Mouse anti Human UBE3A antibody, clone 2F6 recognizes human Ubiquitin-protein ligase E3A, also known as UBE3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP or Renal carcinoma antigen NY-REN-54. UBE3A is an 875 amino acid ~95 kDa E3 ubiquitin-protein ligase. Mutations of the UBE3A gene can lead to the development of Angelman syndrome (AS) a neurodevelopmental disorder characterized by by severe motor and intellectual retardation, hypotonia, ataxia and jerky limb movements (Sadikovic et al. 2014). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 51-151 of human UBE3A
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | ![]() |
0.1 | 10 ug/ml |
Immunohistology - Paraffin 1 | ![]() |
0.1 | 10 ug/ml |
Western Blotting | ![]() |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
References for UBE3A antibody
-
Wolyniec, K. et al. (2012) E6AP ubiquitin ligase regulates PML-induced senescence in Myc-driven lymphomagenesis.
Blood. 120: 822-32. -
Paul, P.J. et al. (2016) Restoration of tumor suppression in prostate cancer by targeting the E3 ligase E6AP.
Oncogene. 35 (48): 6235-45.
- Synonyms
- E6AP
- RRID
- AB_2211795
- UniProt
- Q05086
- Entrez Gene
- UBE3A
MCA3532Z



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