UBE3A antibody | 2F6
Mouse anti Human UBE3A antibody, clone 2F6 recognizes human Ubiquitin-protein ligase E3A, also known as UBE3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP or Renal carcinoma antigen NY-REN-54. UBE3A is an 875 amino acid ~95 kDa E3 ubiquitin-protein ligase.
Mutations of the UBE3A gene can lead to the development of Angelman syndrome (AS) a neurodevelopmental disorder characterized by by severe motor and intellectual retardation, hypotonia, ataxia and jerky limb movements (Sadikovic et al. 2014).
Mutations of the UBE3A gene can lead to the development of Angelman syndrome (AS) a neurodevelopmental disorder characterized by by severe motor and intellectual retardation, hypotonia, ataxia and jerky limb movements (Sadikovic et al. 2014).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 51-151 of human UBE3A
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of UBE3A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Immunohistology - Paraffin 1 | 0.1 | 10 ug/ml | |
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for UBE3A antibody
-
Wolyniec, K. et al. (2012) E6AP ubiquitin ligase regulates PML-induced senescence in Myc-driven lymphomagenesis.
Blood. 120: 822-32. -
Paul, P.J. et al. (2016) Restoration of tumor suppression in prostate cancer by targeting the E3 ligase E6AP.
Oncogene. 35 (48): 6235-45.
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