Tubulin Beta 3 antibody | TU-20

Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin (Jirásek 2002) including neuroblastoma (Prasannan 2000) and ganglioneuroma (Dráberová 1998). Class III beta tubulin is highly expressed in tumors of neuronal origin rather than in non-neuronal tumors (Person 2017).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Baboon Rat Hamster Pig Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by DEAE chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH).
This sequence is widely conserved across species. - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13509
- Entrez Gene
- TUBB3
- GO Terms
- GO:0003924 GTPase activity
- GO:0005198 structural molecule activity
- GO:0005525 GTP binding
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0007018 microtubule-based movement
- GO:0007411 axon guidance
- GO:0051084 'de novo' posttranslational protein folding
- GO:0051258 protein polymerization
- Regulatory
- For research purposes only
Applications of Tubulin Beta 3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | 1/10 | 1/50 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | 5 | 20 ug/ml | |
Western Blotting 2 | 1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2TU-20 recognizes tubulin beta 3 under reducing conditions
- Histology Positive Control Tissue
- Neuronal tissue
Secondary Antibodies Available
Product Specific References
References for Tubulin Beta 3 antibody
-
Dráberová, E. et al. (1998) Expression of class III beta-tubulin in normal and neoplastic human tissues.
Histochem Cell Biol. 109 (3): 231-9. -
Hattermann, K. et al. (2010) The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.
Cancer Res. 70: 3299-308. -
Rosito, M. et al. (2012) CXCL16 Orchestrates Adenosine A3 Receptor and MCP-1/CCL2 Activity to Protect Neurons from Excitotoxic Cell Death in the CNS.
J Neurosci. 32: 3154-63. -
Nicot, A. and DiCicco-Bloom, E. (2001) Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
Proc Natl Acad Sci U S A. 98: 4758-63. -
Pěknicová, J. et al. (2001) Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.
Biol Reprod. 65: 672-9. -
Huang, C.L. et al. (2010) Expression of ERCC1 and class III β-tubulin is associated with the survival of resected stage III non-small cell lung cancer patients treated with induction chemoradiotherapy using carboplatin-taxane.
Exp Ther Med. 1: 445-51. -
Yentur, S.P. et al. (2014) A decrease of regulatory T cells and altered expression of NK receptors are observed in subacute sclerosing panencephalitis.
Viral Immunol. 27 (10): 506-11. -
Alexiou, G.A. et al. (2013) Supratentorial ependymomas in children: Analysis of nine cases.
J Pediatr Neurosci. 8 (1): 15-8. -
Volkov, V.A. et al. (2013) Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules.
Proc Natl Acad Sci U S A. 110 (19): 7708-13. -
Knerlich-Lukoschus, F. et al. (2010) Chemokine expression in the white matter spinal cord precursor niche after force-defined spinal cord contusion injuries in adult rats.
Glia. 58 (8): 916-31. -
Carey, R.G. et al. (2002) Pituitary adenylate cyclase activating polypeptide anti-mitogenic signaling in cerebral cortical progenitors is regulated by p57Kip2-dependent CDK2 activity.
J Neurosci. 22 (5): 1583-91.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra