Tubulin Beta 3 antibody | TU-20

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Mouse anti Tubulin Beta 3:FITC

Mouse anti Tubulin Beta 3

Product Type
Monoclonal Antibody
Clone
TU-20
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2047F IF 0.1 mg
MCA2047 C IF P* WB* 0.1 mg
Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin which is reported to be restricted to neuronal tissue.

Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin including neuroblastoma and ganglioneuroma. Class III beta tubulin has not been detected in tumors of non neuronal origin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Baboon
Rat
Hamster
Pig
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by DEAE chromatography
Preparation
Purified IgG prepared from ascites by DEAE chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH).

This sequence is conserved across all species.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q13509 Related reagents
Entrez Gene
TUBB3 Related reagents
GO Terms
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0007411 axon guidance
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization
Regulatory
For research purposes only

Applications of Tubulin Beta 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 5 20 ug/ml
Western Blotting 2 1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2TU-20 recognizes tubulin beta 3 under reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Neuronal tissue

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Tubulin Beta 3 antibody

  1. Dráberová, E. et al. (1998) Expression of class III beta-tubulin in normal and neoplastic human tissues.
    Histochem Cell Biol. 109 (3): 231-9.
  2. Hattermann, K. et al. (2010) The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.
    Cancer Res. 70: 3299-308.
  3. Rosito, M. et al. (2012) CXCL16 Orchestrates Adenosine A3 Receptor and MCP-1/CCL2 Activity to Protect Neurons from Excitotoxic Cell Death in the CNS.
    J Neurosci. 32: 3154-63.
  4. Nicot, A. and DiCicco-Bloom, E. (2001) Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    Proc Natl Acad Sci U S A. 98: 4758-63.
  5. Pěknicová, J. et al. (2001) Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.
    Biol Reprod. 65: 672-9.
  6. Huang, C.L. et al. (2010) Expression of ERCC1 and class III β-tubulin is associated with the survival of resected stage III non-small cell lung cancer patients treated with induction chemoradiotherapy using carboplatin-taxane.
    Exp Ther Med. 1: 445-51.
  7. Yentur, S.P. et al. (2014) A decrease of regulatory T cells and altered expression of NK receptors are observed in subacute sclerosing panencephalitis.
    Viral Immunol. 27 (10): 506-11.