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Tubulin Beta 3 antibody | TU-20

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Mouse anti Tubulin Beta 3:FITC

Mouse anti Tubulin Beta 3

Product Type
Monoclonal Antibody
Clone
TU-20
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2047F
Datasheet
IF
SDS
0.1 mg loader

MCA2047
Datasheet
C IF P* WB*
SDS
0.1 mg loader

Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin, restricted to neuronal tissue (Leandro-García 2010; Katsetos 2003).

Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin (Jirásek 2002) including neuroblastoma (Prasannan 2000) and ganglioneuroma (Dráberová 1998). Class III beta tubulin is highly expressed in tumors of neuronal origin rather than in non-neuronal tumors (Person 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Baboon
Rat
Hamster
Pig
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by DEAE chromatography
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH).

This sequence is widely conserved across species.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q13509
Entrez Gene
TUBB3
GO Terms
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0007411 axon guidance
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization
Regulatory
For research purposes only

Applications of Tubulin Beta 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 5 20 ug/ml
Western Blotting 2 1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2TU-20 recognizes tubulin beta 3 under reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Neuronal tissue
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunocytochemistry

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Tubulin Beta 3 antibody

  1. Dráberová, E. et al. (1998) Expression of class III beta-tubulin in normal and neoplastic human tissues.
    Histochem Cell Biol. 109 (3): 231-9.
  2. Hattermann, K. et al. (2010) The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.
    Cancer Res. 70: 3299-308.
  3. Rosito, M. et al. (2012) CXCL16 Orchestrates Adenosine A3 Receptor and MCP-1/CCL2 Activity to Protect Neurons from Excitotoxic Cell Death in the CNS.
    J Neurosci. 32: 3154-63.
  4. Nicot, A. and DiCicco-Bloom, E. (2001) Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
    Proc Natl Acad Sci U S A. 98: 4758-63.
  5. Pěknicová, J. et al. (2001) Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.
    Biol Reprod. 65: 672-9.
  6. Huang, C.L. et al. (2010) Expression of ERCC1 and class III β-tubulin is associated with the survival of resected stage III non-small cell lung cancer patients treated with induction chemoradiotherapy using carboplatin-taxane.
    Exp Ther Med. 1: 445-51.
  7. Yentur, S.P. et al. (2014) A decrease of regulatory T cells and altered expression of NK receptors are observed in subacute sclerosing panencephalitis.
    Viral Immunol. 27 (10): 506-11.
  8. Alexiou, G.A. et al. (2013) Supratentorial ependymomas in children: Analysis of nine cases.
    J Pediatr Neurosci. 8 (1): 15-8.
  9. Volkov, V.A. et al. (2013) Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules.
    Proc Natl Acad Sci U S A. 110 (19): 7708-13.
  10. Knerlich-Lukoschus, F. et al. (2010) Chemokine expression in the white matter spinal cord precursor niche after force-defined spinal cord contusion injuries in adult rats.
    Glia. 58 (8): 916-31.
  11. Carey, R.G. et al. (2002) Pituitary adenylate cyclase activating polypeptide anti-mitogenic signaling in cerebral cortical progenitors is regulated by p57Kip2-dependent CDK2 activity.
    J Neurosci. 22 (5): 1583-91.
  12. Zhu, G. et al. (2012) Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.
    Neural Regen Res. 7 (19): 1483-7.
  13. Tarasovetc, E.V. et al. (2021) Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.
    Mol Biol Cell. : mbcE20070461.

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