Tubulin Beta 3 antibody | TU-20
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Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin which is reported to be restricted to neuronal tissue.
Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin including neuroblastoma and ganglioneuroma. Class III beta tubulin has not been detected in tumors of non neuronal origin.
Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin including neuroblastoma and ganglioneuroma. Class III beta tubulin has not been detected in tumors of non neuronal origin.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Baboon Rat Hamster Pig Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by DEAE chromatography
- Preparation
- Purified IgG prepared from ascites by DEAE chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Synthetic peptide, ESESQGPK, corresponding to amino acids 441-448 of human class III beta tubulin coupled to Keyhole Limpet Hemocyanin (KLH).
This sequence is conserved across all species. - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q13509 Related reagents
- Entrez Gene
- TUBB3 Related reagents
- GO Terms
- GO:0003924 GTPase activity
- GO:0005198 structural molecule activity
- GO:0005525 GTP binding
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0007018 microtubule-based movement
- GO:0007411 axon guidance
- GO:0051084 'de novo' posttranslational protein folding
- GO:0051258 protein polymerization
- Regulatory
- For research purposes only
Applications of Tubulin Beta 3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 1/10 | 1/50 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | 5 | 20 ug/ml | |
| Western Blotting 2 | 1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2TU-20 recognizes tubulin beta 3 under reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Neuronal tissue
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Tubulin Beta 3 antibody
-
Dráberová, E. et al. (1998) Expression of class III beta-tubulin in normal and neoplastic human tissues.
Histochem Cell Biol. 109 (3): 231-9. -
Hattermann, K. et al. (2010) The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects.
Cancer Res. 70: 3299-308. -
Rosito, M. et al. (2012) CXCL16 Orchestrates Adenosine A3 Receptor and MCP-1/CCL2 Activity to Protect Neurons from Excitotoxic Cell Death in the CNS.
J Neurosci. 32: 3154-63. -
Nicot, A. and DiCicco-Bloom, E. (2001) Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression.
Proc Natl Acad Sci U S A. 98: 4758-63. -
Pěknicová, J. et al. (2001) Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail.
Biol Reprod. 65: 672-9. -
Huang, C.L. et al. (2010) Expression of ERCC1 and class III β-tubulin is associated with the survival of resected stage III non-small cell lung cancer patients treated with induction chemoradiotherapy using carboplatin-taxane.
Exp Ther Med. 1: 445-51. -
Yentur, S.P. et al. (2014) A decrease of regulatory T cells and altered expression of NK receptors are observed in subacute sclerosing panencephalitis.
Viral Immunol. 27 (10): 506-11.
