Troponin I antibody | 10F4 (110)

Mouse anti Human Troponin I (Cardiac)

Product Type
Monoclonal Antibody
Clone
10F4 (110)
Isotype
IgG2a
Specificity
Troponin I
Region
(CARDIAC)

Product Code Applications Pack Size List Price Your Price Qty
MCA1208
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.25 mg loader
List Price Your Price
loader
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Mouse anti Human Troponin I (Cardiac) antibody, clone 10F4 (110) recognizes aa 34-37 of Troponin I from human heart. There is no cross-reactivity with skeletal muscle troponin I.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Goat
Cat
Rabbit
Bovine
Dog
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Human Cardiac troponin I.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
  1. 1 Zhang et al. 2008.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Troponin I antibody

  1. Marian, A.J. et al. (1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.
    Circ Res. 81 (1): 76-85.
  2. Zhang, J. et al. (2008) Isoproterenol-induced cardiotoxicity in sprague-dawley rats: correlation of reversible and irreversible myocardial injury with release of cardiac troponin T and roles of iNOS in myocardial injury.
    Toxicol Pathol. 36: 277-8.
  3. Filatov, V.L. et al. (1998) Epitope mapping of anti-troponin I monoclonal antibodies.
    Biochem Mol Biol Int. 45 (6): 1179-87.

RRID
AB_2303604
UniProt
P19429
Entrez Gene
TNNI3
GO Terms
GO:0001570 vasculogenesis
GO:0001980 regulation of systemic arterial blood pressure by ischemic conditions
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005861 troponin complex
GO:0006874 cellular calcium ion homeostasis
GO:0019855 calcium channel inhibitor activity
GO:0019901 protein kinase binding
GO:0019904 protein domain specific binding
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031014 troponin T binding
GO:0032780 negative regulation of ATPase activity
GO:0048306 calcium-dependent protein binding
GO:0055010 ventricular cardiac muscle tissue morphogenesis
GO:0060048 cardiac muscle contraction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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