Troponin I antibody | 10F4 (110)
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Mouse anti Human Troponin I (Cardiac) antibody, clone 10F4 (110) recognizes aa 34-37 of Troponin I from human heart. There is no cross-reactivity with skeletal muscle troponin I.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Goat Cat Rabbit Bovine Dog Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human Cardiac troponin I.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19429
- Entrez Gene
- TNNI3
- GO Terms
- GO:0001570 vasculogenesis
- GO:0001980 regulation of systemic arterial blood pressure by ischemic conditions
- GO:0003779 actin binding
- GO:0005829 cytosol
- GO:0005861 troponin complex
- GO:0006874 cellular calcium ion homeostasis
- GO:0019855 calcium channel inhibitor activity
- GO:0019901 protein kinase binding
- GO:0019904 protein domain specific binding
- GO:0030049 muscle filament sliding
- GO:0030172 troponin C binding
- GO:0031014 troponin T binding
- GO:0032780 negative regulation of ATPase activity
- GO:0048306 calcium-dependent protein binding
- GO:0055010 ventricular cardiac muscle tissue morphogenesis
- GO:0060048 cardiac muscle contraction
- Regulatory
- For research purposes only
Applications of Troponin I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Troponin I antibody
-
Marian, A.J. et al. (1997) Expression of a mutant (Arg92Gln) human cardiac troponin T, known to cause hypertrophic cardiomyopathy, impairs adult cardiac myocyte contractility.
Circ Res. 81 (1): 76-85. -
Zhang, J. et al. (2008) Isoproterenol-induced cardiotoxicity in sprague-dawley rats: correlation of reversible and irreversible myocardial injury with release of cardiac troponin T and roles of iNOS in myocardial injury.
Toxicol Pathol. 36: 277-8. -
Filatov, V.L. et al. (1998) Epitope mapping of anti-troponin I monoclonal antibodies.
Biochem Mol Biol Int. 45 (6): 1179-87.
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