Transferrin antibody | HTF-14

100% Secure

No associated image currently available

Mouse anti Human Transferrin (N-Terminal)

Product Type: Monoclonal Antibody
Clone: HTF-14
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
9100-1055	E FN IF WB			0.2 mg

Mouse anti transferrin antibody, clone HTF-14 recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009)
Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep
Pig
Bovine
Dog
Horse
Rabbit
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Purified porcine transferrin.

Approx. Protein Concentrations

IgG concentration 1mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P02787 Related reagents
Entrez Gene: TF Related reagents
GO Terms: GO:0002576 platelet degranulation; GO:0005576 extracellular region; GO:0005769 early endosome; GO:0005770 late endosome; GO:0030141 stored secretory granule; GO:0030139 endocytic vesicle; GO:0005905 coated pit; GO:0006879 cellular iron ion homeostasis; GO:0008199 ferric iron binding; GO:0009925 basal plasma membrane; GO:0010008 endosome membrane; GO:0016324 apical plasma membrane; GO:0030168 platelet activation; GO:0033572 transferrin transport; GO:0048471 perinuclear region of cytoplasm; GO:0055037 recycling endosome; GO:0055085 transmembrane transport
Regulatory: For research purposes only

Applications of Transferrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Functional Assays
Immunofluorescence
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Western Blotting: HTF-14 recognises a band of approximately 77kDa under reducing conditions in human plasma.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Transferrin antibody

Rubikaite, B.I. et al. (1989) [Identification of the segment for binding of transferrin using a cellular receptor].
Mol Biol (Mosk). 23: 765-71.
Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.
Br J Haematol. 62: 21-30.
Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.
Folia Microbiol (Praha). 32: 448-52.
Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.
Biochim Biophys Acta. 969: 158-65.
Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.
Br J Haematol. 59: 435-41.
Penhallow, R.C. et al. (1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.
J Cell Physiol. 128: 251-60.
Funk, W.D. et al. (1990) Expression of the Amino-Terminal Half-Molecule of Human Serum Transferrin in Cultured Cells and Characterization of the Recombinant Protei
Biochemistry. 29: 1654-60.

View more products with TRANSFERRIN specificity