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Transferrin antibody | HTF-14

Mouse anti Human Transferrin (N-Terminal)

Product Type
Monoclonal Antibody
Clone
HTF-14
Isotype
IgG1
Specificity
Transferrin
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
9100-1055
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN IF WB 0.2 mg loader
List Price Your Price
loader

Mouse anti transferrin antibody, clone HTF-14 recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009)
Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Pig
Bovine
Dog
Horse
Rabbit
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified porcine transferrin.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Immunofluorescence
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
HTF-14 recognises a band of approximately 77kDa under reducing conditions in human plasma.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Transferrin antibody

  1. Rubikaite, B.I. et al. (1989) [Identification of the segment for binding of transferrin using a cellular receptor].
    Mol Biol (Mosk). 23: 765-71.
  2. Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.
    Br J Haematol. 62: 21-30.
  3. Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.
    Folia Microbiol (Praha). 32: 448-52.
  4. Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.
    Biochim Biophys Acta. 969: 158-65.
  5. Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.
    Br J Haematol. 59: 435-41.
  6. Penhallow, R.C. et al. (1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.
    J Cell Physiol. 128: 251-60.
  7. Funk, W.D. et al. (1990) Expression of the Amino-Terminal Half-Molecule of Human Serum Transferrin in Cultured Cells and Characterization of the Recombinant Protei
    Biochemistry. 29: 1654-60.

RRID
AB_2202011
UniProt
P02787
Entrez Gene
TF
GO Terms
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005769 early endosome
GO:0005770 late endosome
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0005905 coated pit
GO:0006879 cellular iron ion homeostasis
GO:0008199 ferric iron binding
GO:0009925 basal plasma membrane
GO:0010008 endosome membrane
GO:0016324 apical plasma membrane
GO:0030168 platelet activation
GO:0033572 transferrin transport
GO:0048471 perinuclear region of cytoplasm
GO:0055037 recycling endosome
GO:0055085 transmembrane transport

9100-1055

149617

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