Transferrin antibody | HTF-14
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Mouse anti Human Transferrin (N-Terminal)
- Product Type
- Monoclonal Antibody
- Clone
- HTF-14
- Isotype
- IgG1
- Specificity
- Transferrin
- Region
- (N-TERMINAL)
Antibody Quality and 
Unrivaled 
| Mouse anti transferrin antibody, clone HTF-14 recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009) Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Pig Bovine Dog Horse Rabbit Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified porcine transferrin.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Functional Assays | |
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| Immunofluorescence | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Western Blotting
- HTF-14 recognises a band of approximately 77kDa under reducing conditions in human plasma.
References for Transferrin antibody
-
Rubikaite, B.I. et al. (1989) [Identification of the segment for binding of transferrin using a cellular receptor].
Mol Biol (Mosk). 23: 765-71. -
Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.
Br J Haematol. 62: 21-30. -
Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.
Folia Microbiol (Praha). 32: 448-52. -
Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.
Biochim Biophys Acta. 969: 158-65. -
Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.
Br J Haematol. 59: 435-41. -
Penhallow, R.C. et al. (1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.
J Cell Physiol. 128: 251-60. -
Funk, W.D. et al. (1990) Expression of the Amino-Terminal Half-Molecule of Human Serum Transferrin in Cultured Cells and Characterization of the Recombinant Protei
Biochemistry. 29: 1654-60.
- RRID
- AB_2202011
- UniProt
- P02787
- Entrez Gene
- TF
- GO Terms
- GO:0002576 platelet degranulation
- GO:0005576 extracellular region
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0005905 coated pit
- GO:0006879 cellular iron ion homeostasis
- GO:0008199 ferric iron binding
- View More GO Terms
- GO:0009925 basal plasma membrane
- GO:0010008 endosome membrane
- GO:0016324 apical plasma membrane
- GO:0030168 platelet activation
- GO:0033572 transferrin transport
- GO:0048471 perinuclear region of cytoplasm
- GO:0055037 recycling endosome
- GO:0055085 transmembrane transport
View more products with TRANSFERRIN specificity
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