Thioredoxin antibody | 2B1
Thioredoxin is involved in a number of cellular processes, including activation of the transcription factor NF-KB (Kelleher et al. 2014). Thioredoxin is highly expressed in the glandular cells of the GI tract and uterine tissues, also activated B and T-cells (Rubartelli et al. 1992).
Mouse anti Human thioredoxin antibody, clone 2B1 has been used successfully in a biotinylated format as a detection reagent in a thioredoxin sandwich ELISA as part of a study looking at thioredoxin levels in placental trophoblast cells (Di Trapani et al. 1998), a study which also used the antibody for immunoprecipitation of thioredoxin as well as detection of thioredoxin by western blotting.
Mouse anti Human thioredoxin antibody, clone 2B1 is also useful for the demonstration of thioredoxin expression on formalin fixed, paraffin embedded material by immunohistochemistry (Lincoln et al. 2003). Clone 2B1 has also been used as a capture reagent in a sandwich ELISA for thioredoxin (Perking et al. 1995).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Marmoset
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Recombinant human thioredoxin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0005654 nucleoplasm
- GO:0022900 electron transport chain
- GO:0006350 transcription
- GO:0006810 transport
- GO:0006928 cellular component movement
- GO:0007267 cell-cell signaling
- GO:0008283 cell proliferation
- GO:0009314 response to radiation
- GO:0015949 nucleobase, nucleoside and nucleotide interconversion
- GO:0033158 regulation of protein import into nucleus, translocation
- GO:0043388 positive regulation of DNA binding
- For research purposes only
Applications of Thioredoxin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||F||100 Tests|
Product Specific References
References for Thioredoxin antibody
Perkins, A.V. et al. (1995) Immunocytochemical localization of thioredoxin in human trophoblast and decidua.
Placenta. 16 (7): 635-42.
Lincoln, D.T. et al. (2003) The thioredoxin-thioredoxin reductase system: over-expression in human cancer.
Anticancer Res. 23 (3B): 2425-33.
Stantchev, T.S. et al. (2012) Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection.
Retrovirology. 9: 97.
Ma, X. et al. (2001) Regulation of interferon and retinoic acid-induced cell death activation through thioredoxin reductase.
J Biol Chem. 276: 24843-54.
Ramanathan, R.K. et al. (2007) A Phase I pharmacokinetic and pharmacodynamic study of PX-12, a novel inhibitor of thioredoxin-1, in patients with advanced solid tumors.
Clin Cancer Res. 13: 2109-14.
Arnold, N.B. et al. (2004) Thioredoxin is downstream of Smad7 in a pathway that promotes growth and suppresses cisplatin-induced apoptosis in pancreatic cancer.
Cancer Res. 64: 3599-606.
Kinoshita, T. et al. (2007) Thioredoxin prevents the development and progression of elastase-induced emphysema.
Biochem Biophys Res Commun. 354: 712-9.
Dorion, S. et al. (2002) Activation of the p38 signaling pathway by heat shock involves the dissociation of glutathione S-transferase Mu from Ask1.
J Biol Chem. 277: 30792-7.
Sensi, M. et al. (2005) Immunogenicity without immunoselection: a mutant but functional antioxidant enzyme retained in a human metastatic melanoma and targeted by CD8(+) T cells with a memory phenotype.
Cancer Res. 65: 632-40.
Mamoon, N.M. et al. (2007) Multiple cysteine residues are implicated in Janus kinase 2-mediated catalysis.
Biochemistry. 46: 14810-8.
Agathanggelou, A. et al. (2003) Identification of Novel Gene Expression Targets for the Ras Association Domain Family 1 (RASSF1A) Tumor Suppressor Gene in Non-Small Cell Lung Cancer and Neuroblastoma.
Cancer Res. 63: 5344-51.
Di Trapani, G. et al. (1998) Production and secretion of thioredoxin from transformed human trophoblast cells.
Mol Hum Reprod. 4: 369-75.
Lopata, A. et al. (2001) Expression and localization of thioredoxin during early implantation in the marmoset monkey.
Mol Hum Reprod. 7: 1159-65.
Wang, M.Y. et al. (2011) A redox switch in C-reactive protein modulates activation of endothelial cells.
FASEB J. 25 (9): 3186-96.
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