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Talin-2 antibody | 53.8

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Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 1 Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 2 Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 3 Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 4 Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 5 Anti Human Talin-2 Antibody, clone 53.8 thumbnail image 6
Anti Human Talin-2 Antibody, clone 53.8 gallery image 1
Western blotting analysis of anti talin antibody binding to recombinant talin proteins showing specificity of Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771) for talin-2, not recognizing recombinant human talin-1. In contrast Mouse anti talin-1 antibody, clone 97H6 (MCA4770) does not recognize recombinant human talin-2. Clone 8D4, recognizing both talin isoforms is included.
Originator image
Anti Human Talin-2 Antibody, clone 53.8 gallery image 2
Western blotting analysis of talin-1 and talin-2 expression in murine tissue lysates using Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771) and Mouse anti Human talin-1 antibody, clone 97H6 (MCA4770). (B) brain, (H) heart, (S) spleen, (K) kidney, (Lu) lung, Li (liver), (M) muscle. Originator image.
Anti Human Talin-2 Antibody, clone 53.8 gallery image 3
Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771) used for immunofluorescence staining of frozen murine heart sections highlighting both costameres and intercalated discs. Originator image.
Anti Human Talin-2 Antibody, clone 53.8 gallery image 4
Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771) used for immunofluorescent staining of isolated talin-1 null cells fixed in paraformaldehyde and permeablized with triton X-100. Originator image.
Anti Human Talin-2 Antibody, clone 53.8 gallery image 5
Published customer image:
Mouse anti talin-2 antibody, clone 53.8 (MCA4771) used for the detection of talin-2 in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of talin1 and talin2 in various mouse tissues and their immuno-localisation in muscle and kidney. (A) Western blot of whole tissue lysates (40 μg protein) probed with the talin1- or talin2-specific antibodies shown. Tissues: B, brain; H, heart; S, spleen; K, kidney; Lu, lung; Li, liver; M, skeletal muscle. (B) Cryo-sections of gastrocnemius muscle were immunostained with Mab 97H6 directed against talin1 (a and b) and Mab 68E7 against talin2 (c–f). Talin1 was mostly localised to the myotendinous junction (MTJ) (arrows in a), with weaker staining of costameres (b), while talin2 staining was strong at both MTJ (arrows in c) and costameres (d). In talin2-null muscle, no staining was detected at the MTJ (arrows in e) or at costameres (f). Scale bar = 50 μm. (C) Immuno-staining of paraffin-embedded sections of wild type and talin2 null mouse kidney with antibodies to talin1 (93E12 and 97H6) and talin2 (68E7 and 121A). Staining is present in the glomerulus, tubules and interstitium in the cortex, and the tubules and interstitium in the medulla. There was no staining for talin2 in kidneys from Tln2-null mice.

From: Praekelt U, Kopp PM, Rehm K, Linder S, Bate N, Patel B, Debrand E, Manso AM, Ross RS, Conti F, Zhang MZ, Harris RC, Zent R, Critchley DR, Monkley SJ.
New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Eur J Cell Biol. 2012 Mar;91(3):180-91.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Talin-2 Antibody, clone 53.8 gallery image 6
Published customer image:
Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771GA) used alongside Mouse anti Human talin-1 antibody, clone 97H6 (MCA4770GA) to evaluate talin expression in rabbit heart muscle lysates by western blotting.
Image caption:
Integrins and related proteins in the Dau-treated heart.
(A) Western blot of active β1 integrin, β1D, β1A, talin-1, talin-2, and vinculin in LV from Dau-treated and control hearts. (B) Quantification of (A), n = 4–6, respectively. *p <0.05, **p<0.01. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.

From: Ichikawa Y, Zemljic-Harpf AE, Zhang Z, McKirnan MD, Manso AM, Ross RS, et al. (2017)
Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS ONE 12(5): e0177660.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Talin-2

Product Type
Monoclonal Antibody
Clone
53.8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA4771GA
Datasheet
IF* IP P WB
SDS
0.1 mg loader

MCA4771
Datasheet
IF* IP P WB
SDS
0.2 mg loader

Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity.

Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant Mouse talin-2 (aa2300-2542).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y4G6
Entrez Gene
TLN2
GO Terms
GO:0001726 ruffle
GO:0003779 actin binding
GO:0007155 cell adhesion
GO:0005158 insulin receptor binding
GO:0005200 structural constituent of cytoskeleton
GO:0005737 cytoplasm
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007043 cell-cell junction assembly
GO:0015629 actin cytoskeleton
GO:0045202 synapse
Regulatory
For research purposes only

Applications of Talin-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1
Immunohistology - Paraffin 1/25 1/100
Immunoprecipitation
Western Blotting
  1. 1Methanol treatment is required prior to antibody staining
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Source Reference

  1. Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
    Eur J Cell Biol. 91 (3): 180-91.

References for Talin-2 antibody

  1. Liu, J. et al. (2015) Talin determines the nanoscale architecture of focal adhesions.
    Proc Natl Acad Sci U S A. 112 (35): E4864-73.
  2. Prince, H.E. & Yeh, C. (2013) Reactivity of human IgM binding murine monoclonal 6B6C1 (IgG2a) with other murine monoclonal IgG antibodies.
    J Clin Lab Anal. 27 (1): 27-30.
  3. Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
    J Cell Sci. 129 (19): 3661-74.
  4. Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
    PLoS One. 12 (5): e0177660.

Further Reading

  1. Critchley, D.R. & Gingras, A.R. (2008) Talin at a glance.
    J Cell Sci. 121 (Pt 9): 1345-7.
  2. Monkley SJ et al. (2001) Analysis of the mammalian talin2 gene TLN2.
    Biochem Biophys Res Commun. 286 (5): 880-5.

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