Talin-2 antibody | 53.8
Originator image
Mouse anti talin-2 antibody, clone 53.8 (121A) used for the detection of talin-2 in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of talin1 and talin2 in various mouse tissues and their immuno-localisation in muscle and kidney. (A) Western blot of whole tissue lysates (40 μg protein) probed with the talin1- or talin2-specific antibodies shown. Tissues: B, brain; H, heart; S, spleen; K, kidney; Lu, lung; Li, liver; M, skeletal muscle. (B) Cryo-sections of gastrocnemius muscle were immunostained with Mab 97H6 directed against talin1 (a and b) and Mab 68E7 against talin2 (c–f). Talin1 was mostly localised to the myotendinous junction (MTJ) (arrows in a), with weaker staining of costameres (b), while talin2 staining was strong at both MTJ (arrows in c) and costameres (d). In talin2-null muscle, no staining was detected at the MTJ (arrows in e) or at costameres (f). Scale bar = 50 μm. (C) Immuno-staining of paraffin-embedded sections of wild type and talin2 null mouse kidney with antibodies to talin1 (93E12 and 97H6) and talin2 (68E7 and 121A). Staining is present in the glomerulus, tubules and interstitium in the cortex, and the tubules and interstitium in the medulla. There was no staining for talin2 in kidneys from Tln2-null mice.
From: Praekelt U, Kopp PM, Rehm K, Linder S, Bate N, Patel B, Debrand E, Manso AM, Ross RS, Conti F, Zhang MZ, Harris RC, Zent R, Critchley DR, Monkley SJ. New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Eur J Cell Biol. 2012 Mar;91(3):180-91.
Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771GA) used alongside Mouse anti Human talin-1 antibody, clone 97H6 (MCA4770GA) to evaluate talin expression in rabbit heart muscle lysates by western blotting.
Image caption:
Integrins and related proteins in the Dau-treated heart.
(A) Western blot of active β1 integrin, β1D, β1A, talin-1, talin-2, and vinculin in LV from Dau-treated and control hearts. (B) Quantification of (A), n = 4–6, respectively. *p <0.05, **p<0.01. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.
From: Ichikawa Y, Zemljic-Harpf AE, Zhang Z, McKirnan MD, Manso AM, Ross RS, et al. (2017)
Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS ONE 12(5): e0177660.
Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity.
Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.
Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant Mouse talin-2 (aa2300-2542).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q9Y4G6 Related reagents
- Entrez Gene
- TLN2 Related reagents
- GO Terms
- GO:0001726 ruffle
- GO:0003779 actin binding
- GO:0007155 cell adhesion
- GO:0005158 insulin receptor binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0005737 cytoplasm
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007043 cell-cell junction assembly
- GO:0015629 actin cytoskeleton
- GO:0045202 synapse
- Regulatory
- For research purposes only
Applications of Talin-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence 1 | |||
| Immunohistology - Paraffin | 1/25 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting |
- 1Methanol treatment is required prior to antibody staining
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Source Reference
-
Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Eur J Cell Biol. 91 (3): 180-91.
References for Talin-2 antibody
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Liu, J. et al. (2015) Talin determines the nanoscale architecture of focal adhesions.
Proc Natl Acad Sci U S A. 112 (35): E4864-73. -
Prince, H.E. & Yeh, C. (2013) Reactivity of human IgM binding murine monoclonal 6B6C1 (IgG2a) with other murine monoclonal IgG antibodies.
J Clin Lab Anal. 27 (1): 27-30. -
Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
J Cell Sci. 129 (19): 3661-74. -
Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS One. 12 (5): e0177660.
Further Reading
-
Critchley, D.R. & Gingras, A.R. (2008) Talin at a glance.
J Cell Sci. 121 (Pt 9): 1345-7. -
Monkley SJ et al. (2001) Analysis of the mammalian talin2 gene TLN2.
Biochem Biophys Res Commun. 286 (5): 880-5.
