Talin-2 antibody | 53.8






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WB IF P ResetMouse anti Human Talin-2
- Product Type
- Monoclonal Antibody
- Clone
- 53.8
- Isotype
- IgG2b
- Specificity
- Talin-2
Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity. Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant Mouse talin-2 (aa2300-2542).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence 1 | ![]() |
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Immunohistology - Paraffin | ![]() |
1/25 | 1/100 |
Immunoprecipitation | ![]() |
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Western Blotting | ![]() |
- 1Methanol treatment is required prior to antibody staining
- Histology Positive Control Tissue
- Tonsil
Source Reference
-
Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Eur J Cell Biol. 91 (3): 180-91.
References for Talin-2 antibody
-
Liu, J. et al. (2015) Talin determines the nanoscale architecture of focal adhesions.
Proc Natl Acad Sci U S A. 112 (35): E4864-73. -
Prince, H.E. & Yeh, C. (2013) Reactivity of human IgM binding murine monoclonal 6B6C1 (IgG2a) with other murine monoclonal IgG antibodies.
J Clin Lab Anal. 27 (1): 27-30. -
Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
J Cell Sci. 129 (19): 3661-74. -
Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS One. 12 (5): e0177660.
Further Reading
-
Critchley, D.R. & Gingras, A.R. (2008) Talin at a glance.
J Cell Sci. 121 (Pt 9): 1345-7. -
Monkley SJ et al. (2001) Analysis of the mammalian talin2 gene TLN2.
Biochem Biophys Res Commun. 286 (5): 880-5.
- RRID
- AB_10672561
- UniProt
- Q9Y4G6
- Entrez Gene
- TLN2
- GO Terms
- GO:0001726 ruffle
- GO:0003779 actin binding
- GO:0007155 cell adhesion
- GO:0005158 insulin receptor binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0005737 cytoplasm
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
- GO:0007016 cytoskeletal anchoring at plasma membrane
- View More GO Terms
- GO:0007043 cell-cell junction assembly
- GO:0015629 actin cytoskeleton
- GO:0045202 synapse
MCA4771


MCA4771GA


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