Talin-2 antibody | 53.8

Mouse anti Human Talin-2

Product Type
Monoclonal Antibody
Clone
53.8
Isotype
IgG2b
Specificity
Talin-2

Product Code Applications Pack Size List Price Your Price Qty
MCA4771
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF * IP P WB 0.2 mg loader
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loader
MCA4771GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF * IP P WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity.

Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant Mouse talin-2 (aa2300-2542).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1
Immunohistology - Paraffin 1/25 1/100
Immunoprecipitation
Western Blotting
  1. 1Methanol treatment is required prior to antibody staining
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

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Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Source Reference

  1. Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
    Eur J Cell Biol. 91 (3): 180-91.

References for Talin-2 antibody

  1. Liu, J. et al. (2015) Talin determines the nanoscale architecture of focal adhesions.
    Proc Natl Acad Sci U S A. 112 (35): E4864-73.
  2. Prince, H.E. & Yeh, C. (2013) Reactivity of human IgM binding murine monoclonal 6B6C1 (IgG2a) with other murine monoclonal IgG antibodies.
    J Clin Lab Anal. 27 (1): 27-30.
  3. Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
    J Cell Sci. 129 (19): 3661-74.
  4. Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
    PLoS One. 12 (5): e0177660.

Further Reading

  1. Critchley, D.R. & Gingras, A.R. (2008) Talin at a glance.
    J Cell Sci. 121 (Pt 9): 1345-7.
  2. Monkley SJ et al. (2001) Analysis of the mammalian talin2 gene TLN2.
    Biochem Biophys Res Commun. 286 (5): 880-5.

RRID
AB_10672561
UniProt
Q9Y4G6
Entrez Gene
TLN2
GO Terms
GO:0001726 ruffle
GO:0003779 actin binding
GO:0007155 cell adhesion
GO:0005158 insulin receptor binding
GO:0005200 structural constituent of cytoskeleton
GO:0005737 cytoplasm
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007043 cell-cell junction assembly
GO:0015629 actin cytoskeleton
GO:0045202 synapse
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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