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Talin-1 antibody | 97H6

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Anti Human Talin-1 Antibody, clone 97H6 thumbnail image 1 Anti Human Talin-1 Antibody, clone 97H6 thumbnail image 2 Anti Human Talin-1 Antibody, clone 97H6 thumbnail image 3
Anti Human Talin-1 Antibody, clone 97H6 gallery image 1
Murine embryonic fibroblast stained with Mouse anti talin 1 antibody, clone 97H6 (MCA4770) and Mouse anti talin-2 antibody, clone 68E7 by immunofluorescence demonstrating partial overlapping expression. Originator image.
Anti Human Talin-1 Antibody, clone 97H6 gallery image 2
Published customer image:
Mouse anti Human talin-1 antibody, clone 97H6 (MCA4770GA) used alongside Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771GA) to evaluate talin expression in rabbit heart muscle lysates by western blotting.
Image caption:
Integrins and related proteins in the Dau-treated heart.
(A) Western blot of active β1 integrin, β1D, β1A, talin-1, talin-2, and vinculin in LV from Dau-treated and control hearts. (B) Quantification of (A), n = 4–6, respectively. *p <0.05, **p<0.01. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.

From: Ichikawa Y, Zemljic-Harpf AE, Zhang Z, McKirnan MD, Manso AM, Ross RS, et al. (2017)
Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS ONE 12(5): e0177660.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Talin-1 Antibody, clone 97H6 gallery image 3
Subcellular localization o f talin1 and talin2 in cultured cells. Three cells types mouse NIH3T3, undifferentiated C2C12 cells and mouse embryo fibroblasts were fixed and stained for either talin1 using Mouse anti talin-1 antibody, clone 97H6 (MCA4770), top row or talin2 using Mouse anti Talin-2 antibody, clone 68E7 bottom row, both in green and actin in red. Cells were fixed and permeabilized using methanol. Originator image.

Mouse anti Human Talin-1

Product Type
Monoclonal Antibody
Clone
97H6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4770
Datasheet
IF* IP P* WB
SDS
0.2 mg loader

MCA4770GA
Datasheet
IF* IP P* WB
SDS
0.1 mg loader

Mouse anti Human Talin-1 antibody, clone 97H6 recognizes an epitope within the region of amino acids 482-655 of humanTalin-1, a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
his-tagged polypeptide containing residues 489–911 of mouse talin1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the NS0 murine myeloma.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y490
Entrez Gene
TLN1
GO Terms
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005158 insulin receptor binding
GO:0005200 structural constituent of cytoskeleton
GO:0005813 centrosome
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007043 cell-cell junction assembly
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017166 vinculin binding
GO:0030168 platelet activation
GO:0030274 LIM domain binding
GO:0032587 ruffle membrane
GO:0043231 intracellular membrane-bounded organelle
Regulatory
For research purposes only

Applications of Talin-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1
Immunohistology - Paraffin 2 1/25 1/100 Pack Size: 0.2 mg
1/200 Pack Size: 0.1 mg
Immunoprecipitation
Western Blotting
  1. 1Methanol treatment is required prior to antibody staining
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

Source Reference

  1. Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
    Eur J Cell Biol. 91: 180-91.

References for Talin-1 antibody

  1. Kopp, P.M. et al. (2010) Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.
    Eur J Cell Biol. 89(9):661-73.
  2. Debrand, E. et al. (2012) Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.
    Biochem Biophys Res Commun. 426: 190-5.
  3. Manso, A.M. et al. (2013) Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
    J Biol Chem. 288: 4252-64
  4. Liu, J. et al. (2011) Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.
    Mol Cell Biol. 31: 3366-77.
  5. Monkley, S.J. et al. (2011) Endothelial cell talin1 is essential for embryonic angiogenesis.
    Dev Biol. 349: 494-502.
  6. Ketscher, A. et al. (2014) LSD1 controls metastasis of androgen-independent prostate cancer cells through PXN and LPAR6.
    Oncogenesis. 3: e120.
  7. Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
    J Cell Sci. 129 (19): 3661-74.
  8. Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
    PLoS One. 12 (5): e0177660.

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