Talin-1 antibody | 97H6
Mouse anti Human talin-1 antibody, clone 97H6 (MCA4770GA) used alongside Mouse anti Human talin-2 antibody, clone 53.8 (MCA4771GA) to evaluate talin expression in rabbit heart muscle lysates by western blotting.
Image caption:
Integrins and related proteins in the Dau-treated heart.
(A) Western blot of active β1 integrin, β1D, β1A, talin-1, talin-2, and vinculin in LV from Dau-treated and control hearts. (B) Quantification of (A), n = 4–6, respectively. *p <0.05, **p<0.01. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.
From: Ichikawa Y, Zemljic-Harpf AE, Zhang Z, McKirnan MD, Manso AM, Ross RS, et al. (2017)
Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS ONE 12(5): e0177660.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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IF WB ResetMouse anti Human Talin-1
- Product Type
- Monoclonal Antibody
- Clone
- 97H6
- Isotype
- IgG1
- Specificity
- Talin-1
Antibody Quality and 
Unrivaled 
| Mouse anti Human Talin-1 antibody, clone 97H6 recognizes an epitope within the region of amino acids 482-655 of humanTalin-1, a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- his-tagged polypeptide containing residues 489–911 of mouse talin1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the NS0 murine myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence 1 |  |
||
| Immunohistology - Paraffin 2 | |
1/25 | 1/100 Pack Size: 0.2 mg 1/200 Pack Size: 0.1 mg |
| Immunoprecipitation | |
||
| Western Blotting | |
- 1Methanol treatment is required prior to antibody staining
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
Source Reference
-
Praekelt, U. et al. (2012) New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Eur J Cell Biol. 91: 180-91.
References for Talin-1 antibody
-
Kopp, P.M. et al. (2010) Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.
Eur J Cell Biol. 89(9):661-73. -
Debrand, E. et al. (2012) Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.
Biochem Biophys Res Commun. 426: 190-5. -
Manso, A.M. et al. (2013) Talin1 has unique expression versus talin 2 in the heart and modifies the hypertrophic response to pressure overload.
J Biol Chem. 288: 4252-64 -
Liu, J. et al. (2011) Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.
Mol Cell Biol. 31: 3366-77. -
Monkley, S.J. et al. (2011) Endothelial cell talin1 is essential for embryonic angiogenesis.
Dev Biol. 349: 494-502. -
Ketscher, A. et al. (2014) LSD1 controls metastasis of androgen-independent prostate cancer cells through PXN and LPAR6.
Oncogenesis. 3: e120. -
Qi, L. et al. (2016) Talin2-mediated traction force drives matrix degradation and cell invasion.
J Cell Sci. 129 (19): 3661-74. -
Ichikawa, Y. et al. (2017) Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits.
PLoS One. 12 (5): e0177660.
View The Latest Product References
- RRID
- AB_10671591
- UniProt
- Q9Y490
- Entrez Gene
- TLN1
- GO Terms
- GO:0002576 platelet degranulation
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0007155 cell adhesion
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005158 insulin receptor binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0005813 centrosome
- View More GO Terms
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
- GO:0006928 cellular component movement
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007043 cell-cell junction assembly
- GO:0007411 axon guidance
- GO:0015629 actin cytoskeleton
- GO:0017166 vinculin binding
- GO:0030168 platelet activation
- GO:0030274 LIM domain binding
- GO:0032587 ruffle membrane
- GO:0043231 intracellular membrane-bounded organelle
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