Synaptophysin antibody | SY38

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Mouse anti Synaptophysin

Product Type: Monoclonal Antibody
Clone: SY38
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
8479-0004	C P WB			25 µg

Mouse anti Human synaptophysin antibody, clone SY38 recognizes synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid ~38kDa multi pass transmembrane glycoprotein containing a single MARVEL domain. Synaptophysin is characteristic of small neurosecretory vesicles and is also present in neuroendocrine cells of neuronal and epithelial phenotype (UniProt: P08247). Synaptophysin is widely used as a marker for nerve terminals and for differentiating neuroendocrine tumours.

Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Mouse
Bovine
Western grey kangaroo

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A.

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide, 0.5% Bovine Serum Albumin.

Immunogen

Synaptophysin presynaptic vesicles.

Approx. Protein Concentrations

0.05 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P08247 Related reagents
Entrez Gene: SYP Related reagents
GO Terms: GO:0005509 calcium ion binding; GO:0005215 transporter activity; GO:0015485 cholesterol binding; GO:0016188 synaptic vesicle maturation; GO:0019717 synaptosome; GO:0030054 cell junction; GO:0030285 integral to synaptic vesicle membrane; GO:0048169 regulation of long-term neuronal synaptic plasticity; GO:0048172 regulation of short-term neuronal synaptic plasticity; GO:0048499 synaptic vesicle membrane organization
Regulatory: For research purposes only

Applications of Synaptophysin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Synaptophysin antibody

Thiele, C. et al. (2000) Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
Nat Cell Biol. 2: 42-9.
Cavalla, P. and Schiffer, D. (2001) Neuroendocrine tumors in the brain.
Ann Oncol. 12 Suppl 2:S131-4.
Leube, R.E. (1995) The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
J Cell Sci. 108: 883-94.
Kahle, P.J. et al. (2000) Subcellular localization of wild-type and Parkinson's disease-associated mutant alpha -synuclein in human and transgenic mouse brain.
J Neurosci. 20: 6365-73.
González-Jamett, A.M. et al. (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.
J Neurosci. 30: 10683-91.
Kasprzak, A. et al. (2007) Selected markers (chromogranin A, neuron-specific enolase, synaptophysin, protein gene product 9.5) in diagnosis and prognosis of neuroendocrine pulmonary tumours.
Pol J Pathol. 58: 23-33.
Spiwoks-Becker, I. et al. (2001) Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
Neuroscience. 107: 127-42.
Etherington, S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
J Zool. Jun 26 [Epub ahead of print]
Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
Brain. 136 (Pt 1): 147-67.
Grossi, A.B. et al. (2013) Histologic and immunohistochemical classification of 41 bovine adrenal gland neoplasms.
Vet Pathol. 50 (3): 534-42.