Synaptophysin antibody | SY38

Mouse anti Synaptophysin

Product Type
Monoclonal Antibody
Clone
SY38
Isotype
IgG1
Specificity
Synaptophysin

Product Code Applications Pack Size List Price Your Price Qty
8479-0004
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SDS Safety Datasheet SDS
C P WB 25 µg loader
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loader
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Mouse anti Human synaptophysin antibody, clone SY38 recognizes synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid ~38kDa multi pass transmembrane glycoprotein containing a single MARVEL domain. Synaptophysin is characteristic of small neurosecretory vesicles and is also present in neuroendocrine cells of neuronal and epithelial phenotype (UniProt: P08247). Synaptophysin is widely used as a marker for nerve terminals and for differentiating neuroendocrine tumours.

Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Bovine
Western grey kangaroo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synaptophysin presynaptic vesicles.
Approx. Protein Concentrations
0.05 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Synaptophysin antibody

  1. Zhang, S. et al. (2009) Bronchial Carcinoid Tumor in a Young Man with Neurofibromatosis. Case Report and Review of the Literature
    N A J Med Sci. 2: 17-20.
  2. Thiele, C. et al. (2000) Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
    Nat Cell Biol. 2: 42-9.
  3. Cavalla, P. and Schiffer, D. (2001) Neuroendocrine tumors in the brain.
    Ann Oncol. 12 Suppl 2:S131-4.
  4. Leube, R.E. (1995) The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
    J Cell Sci. 108: 883-94.
  5. Kahle, P.J. et al. (2000) Subcellular localization of wild-type and Parkinson's disease-associated mutant alpha -synuclein in human and transgenic mouse brain.
    J Neurosci. 20: 6365-73.
  6. González-Jamett, A.M. et al. (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.
    J Neurosci. 30: 10683-91.
  7. Kasprzak, A. et al. (2007) Selected markers (chromogranin A, neuron-specific enolase, synaptophysin, protein gene product 9.5) in diagnosis and prognosis of neuroendocrine pulmonary tumours.
    Pol J Pathol. 58: 23-33.
  8. Spiwoks-Becker, I. et al. (2001) Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
    Neuroscience. 107: 127-42.
  9. View The Latest Product References
  10. Etherington, S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
    J Zool. Jun 26 [Epub ahead of print]
  11. Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
    Brain. 136 (Pt 1): 147-67.
  12. Grossi, A.B. et al. (2013) Histologic and immunohistochemical classification of 41 bovine adrenal gland neoplasms.
    Vet Pathol. 50 (3): 534-42.
  13. Hassan, R. et al. (2019) The prospective role of mesenchymal stem cells exosomes on circumvallate taste buds in induced Alzheimer's disease of ovariectomized albino rats: (Light and transmission electron microscopic study).
    Arch Oral Biol. 110: 104596.
  14. Paasila, P.J. et al. (2021) Ground state depletion microscopy as a tool for studying microglia-synapse interactions.
    J Neurosci Res. 99 (6): 1515-32.
  15. Gudi, V. et al. (2017) Synaptophysin Is a Reliable Marker for Axonal Damage.
    J Neuropathol Exp Neurol. 76 (2): 109-25.
  16. Hassan, R. et al. (2020) The prospective role of mesenchymal stem cells exosomes on circumvallate taste buds in induced Alzheimer's disease of ovariectomized albino rats: (Light and transmission electron microscopic study).
    Arch Oral Biol. 110: 104596.
  17. Gingele, S. et al. (2020) Delayed Demyelination and Impaired Remyelination in Aged Mice in the Cuprizone Model.
    Cells. 9 (4): 945

Further Reading

  1. Wiedenmann, B. et sal. (1991) Synaptophysin. A widespread constituent of small neuroendocrine vesicles and a new tool in tumor diagnosis.
    Acta Oncol. 30: 435-40.

Immunohistology - Paraffin

Western Blotting

RRID
AB_2286948
UniProt
P08247
Entrez Gene
SYP
GO Terms
GO:0005509 calcium ion binding
GO:0005215 transporter activity
GO:0015485 cholesterol binding
GO:0016188 synaptic vesicle maturation
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0030285 integral to synaptic vesicle membrane
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0048499 synaptic vesicle membrane organization
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