Synaptophysin antibody | SY38

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Mouse anti Synaptophysin

Product Type
Monoclonal Antibody
Clone
SY38
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8479-0004 C P WB 25 µg
Mouse anti Human synaptophysin antibody, clone SY38 recognizes synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid ~38kDa multi pass transmembrane glycoprotein containing a single MARVEL domain. Synaptophysin is characteristic of small neurosecretory vesicles and is also present in neuroendocrine cells of neuronal and epithelial phenotype (UniProt: P08247). Synaptophysin is widely used as a marker for nerve terminals and for differentiating neuroendocrine tumours.

Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Bovine
Western grey kangaroo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
Immunogen
Synaptophysin presynaptic vesicles.
Approx. Protein Concentrations
0.05 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08247 Related reagents
Entrez Gene
SYP Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005215 transporter activity
GO:0015485 cholesterol binding
GO:0016188 synaptic vesicle maturation
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0030285 integral to synaptic vesicle membrane
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0048172 regulation of short-term neuronal synaptic plasticity
GO:0048499 synaptic vesicle membrane organization
Regulatory
For research purposes only

Applications of Synaptophysin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Synaptophysin antibody

  1. Thiele, C. et al. (2000) Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
    Nat Cell Biol. 2: 42-9.
  2. Cavalla, P. and Schiffer, D. (2001) Neuroendocrine tumors in the brain.
    Ann Oncol. 12 Suppl 2:S131-4.
  3. Leube, R.E. (1995) The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
    J Cell Sci. 108: 883-94.
  4. Kahle, P.J. et al. (2000) Subcellular localization of wild-type and Parkinson's disease-associated mutant alpha -synuclein in human and transgenic mouse brain.
    J Neurosci. 20: 6365-73.
  5. González-Jamett, A.M. et al. (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.
    J Neurosci. 30: 10683-91.
  6. Kasprzak, A. et al. (2007) Selected markers (chromogranin A, neuron-specific enolase, synaptophysin, protein gene product 9.5) in diagnosis and prognosis of neuroendocrine pulmonary tumours.
    Pol J Pathol. 58: 23-33.
  7. Spiwoks-Becker, I. et al. (2001) Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
    Neuroscience. 107: 127-42.
  8. Etherington, S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
    J Zool. Jun 26 [Epub ahead of print]
  9. Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
    Brain. 136 (Pt 1): 147-67.
  10. Grossi, A.B. et al. (2013) Histologic and immunohistochemical classification of 41 bovine adrenal gland neoplasms.
    Vet Pathol. 50 (3): 534-42.

Further Reading

  1. Wiedenmann, B. et sal. (1991) Synaptophysin. A widespread constituent of small neuroendocrine vesicles and a new tool in tumor diagnosis.
    Acta Oncol. 30: 435-40.