Synaptophysin antibody | SY38

Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse Bovine Western grey kangaroo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
- Immunogen
- Synaptophysin presynaptic vesicles.
- Approx. Protein Concentrations
- 0.05 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08247
- Entrez Gene
- SYP
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005215 transporter activity
- GO:0015485 cholesterol binding
- GO:0016188 synaptic vesicle maturation
- GO:0019717 synaptosome
- GO:0030054 cell junction
- GO:0030285 integral to synaptic vesicle membrane
- GO:0048169 regulation of long-term neuronal synaptic plasticity
- GO:0048172 regulation of short-term neuronal synaptic plasticity
- GO:0048499 synaptic vesicle membrane organization
- Regulatory
- For research purposes only
Applications of Synaptophysin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting |
Secondary Antibodies Available
Product Specific References
References for Synaptophysin antibody
-
Thiele, C. et al. (2000) Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
Nat Cell Biol. 2: 42-9. -
Cavalla, P. and Schiffer, D. (2001) Neuroendocrine tumors in the brain.
Ann Oncol. 12 Suppl 2:S131-4. -
Leube, R.E. (1995) The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
J Cell Sci. 108: 883-94. -
Kahle, P.J. et al. (2000) Subcellular localization of wild-type and Parkinson's disease-associated mutant alpha -synuclein in human and transgenic mouse brain.
J Neurosci. 20: 6365-73. -
González-Jamett, A.M. et al. (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.
J Neurosci. 30: 10683-91. -
Kasprzak, A. et al. (2007) Selected markers (chromogranin A, neuron-specific enolase, synaptophysin, protein gene product 9.5) in diagnosis and prognosis of neuroendocrine pulmonary tumours.
Pol J Pathol. 58: 23-33. -
Spiwoks-Becker, I. et al. (2001) Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
Neuroscience. 107: 127-42. -
Etherington, S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
J Zool. Jun 26 [Epub ahead of print] -
Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
Brain. 136 (Pt 1): 147-67. -
Grossi, A.B. et al. (2013) Histologic and immunohistochemical classification of 41 bovine adrenal gland neoplasms.
Vet Pathol. 50 (3): 534-42.
Further Reading
-
Wiedenmann, B. et sal. (1991) Synaptophysin. A widespread constituent of small neuroendocrine vesicles and a new tool in tumor diagnosis.
Acta Oncol. 30: 435-40.
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