Synaptophysin antibody | SY38
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Mouse anti Human synaptophysin antibody, clone SY38 recognizes synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid ~38kDa multi pass transmembrane glycoprotein containing a single MARVEL domain. Synaptophysin is characteristic of small neurosecretory vesicles and is also present in neuroendocrine cells of neuronal and epithelial phenotype (UniProt: P08247). Synaptophysin is widely used as a marker for nerve terminals and for differentiating neuroendocrine tumours.
Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).
Mouse anti Human synaptophysin antibody, clone SY38 has been used to detect synaptophysin in a number of neuronal and adrenal tumours including, pheochromocytomas, ganglioneuromas (Pace et al. 2002), neuroendocrine tumours of epithelial origin; pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids and medullary carcinoma of the thyroid (Wiedenmann et al. 1986).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse Bovine Western grey kangaroo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
- Immunogen
- Synaptophysin presynaptic vesicles.
- Approx. Protein Concentrations
- 0.05 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P08247 Related reagents
- Entrez Gene
- SYP Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005215 transporter activity
- GO:0015485 cholesterol binding
- GO:0016188 synaptic vesicle maturation
- GO:0019717 synaptosome
- GO:0030054 cell junction
- GO:0030285 integral to synaptic vesicle membrane
- GO:0048169 regulation of long-term neuronal synaptic plasticity
- GO:0048172 regulation of short-term neuronal synaptic plasticity
- GO:0048499 synaptic vesicle membrane organization
- Regulatory
- For research purposes only
Applications of Synaptophysin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Synaptophysin antibody
-
Thiele, C. et al. (2000) Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles.
Nat Cell Biol. 2: 42-9. -
Cavalla, P. and Schiffer, D. (2001) Neuroendocrine tumors in the brain.
Ann Oncol. 12 Suppl 2:S131-4. -
Leube, R.E. (1995) The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
J Cell Sci. 108: 883-94. -
Kahle, P.J. et al. (2000) Subcellular localization of wild-type and Parkinson's disease-associated mutant alpha -synuclein in human and transgenic mouse brain.
J Neurosci. 20: 6365-73. -
González-Jamett, A.M. et al. (2010) The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.
J Neurosci. 30: 10683-91. -
Kasprzak, A. et al. (2007) Selected markers (chromogranin A, neuron-specific enolase, synaptophysin, protein gene product 9.5) in diagnosis and prognosis of neuroendocrine pulmonary tumours.
Pol J Pathol. 58: 23-33. -
Spiwoks-Becker, I. et al. (2001) Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
Neuroscience. 107: 127-42. -
Etherington, S. J. et al. (2016) Heterochronic neuromuscular junction development in an Australian marsupial (Macropus fuliginosus)
J Zool. Jun 26 [Epub ahead of print] -
Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
Brain. 136 (Pt 1): 147-67. -
Grossi, A.B. et al. (2013) Histologic and immunohistochemical classification of 41 bovine adrenal gland neoplasms.
Vet Pathol. 50 (3): 534-42.
Further Reading
-
Wiedenmann, B. et sal. (1991) Synaptophysin. A widespread constituent of small neuroendocrine vesicles and a new tool in tumor diagnosis.
Acta Oncol. 30: 435-40.
