Syk antibody | SYK-01

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Mouse anti Syk

Product Type
Monoclonal Antibody
Clone
SYK-01
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2383 IP WB 0.1 mg
Mouse anti Human Syk antibody, clone SYK-01 recognizes protein tyrosine kinase p72syk (syk), a ~72kDa non-receptor tyrosine kinase, critical for immunoreceptor signaling in haematopoietic cells, but also expressed by nonhaematopoietic cells, including fibroblasts, endothelial cells and hepatocytes.

Syk plays an important role in mast cell response, initiated by the aggregation of the IgE high affinity receptor Fc epsilon R1. Phosphorylation of ITAMs within Fc receptor R1 by another tyrosine kinase lyn, results in the recruitment of syk, which is then responsible for the further phosphorylation of numerous target proteins within many different signal transduction pathways.

Syk also plays a major role in B cell receptor-mediated apoptosis, proliferation and haematopoiesis. Syk associates with protein kinase C alpha during platelet activation.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant fragment corresponding to amino acids 5 - 360 of human syk.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P43405 Related reagents
Entrez Gene
SYK Related reagents
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0006461 protein complex assembly
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0009887 organ morphogenesis
GO:0008283 cell proliferation
GO:0030168 platelet activation
GO:0030593 neutrophil chemotaxis
GO:0042101 T cell receptor complex
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of Syk antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2383 detects a band of approximately 72kDa in Ramos & RBL cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunoprecipitation

Western Blotting

Product Specific References

References for Syk antibody

  1. Amoui, M. et al. (1997) Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors.
    Eur. J. Immunol. 27(1): 321-328.
  2. Tolar, P. et al. (1997) Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells.
    Eur. J. Immunol. 27(12): 3389-3397.
  3. Manetz, T.S. et al. (2001) Vav1 regulates phospholipase C gamma activation and calcium responses in mast cells.
    Mol. Cell. Biol. 21(11): 3763-3774.
  4. Swann, P.G.et al. (1999) Requirement for a negative charge at Threonine 60 of the FcR gamma for complete activation of Syk.
    J. Biol. Chem. 274(33): 23068-23077.
  5. Arudchandran, R.et al. (2000) The src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH2-terminal kinase 1.
    J. Exp. Med. 191(1): 47-59.