Sulfatase 2 antibody | 2B4

Mouse anti Human Sulfatase 2 (C-Terminal)

Product Type
Monoclonal Antibody
Clone
2B4
Isotype
IgG1
Specificity
Sulfatase 2
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
MCA5692GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
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loader
MCA5692T
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SDS Safety Datasheet SDS
E P * WB 25 µg loader
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loader
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Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Sulf-2 is linked with several cancers, including breast, hepatocellular, pancreatic and non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010).

Mature human Sulf-2 is a heterodimer consisting of a 50 kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75 kDa N-terminal subunit which contains the catalytic centre.

Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide.
Carrier Free
Yes
Immunogen
Recombinant human Sulf-2.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
Western Blotting
Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.

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References for Sulfatase 2 antibody

  1. Lemjabbar-Alaoui, H. et al. (2010) Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis.
    Oncogene. 29 (5): 635-46.
  2. Lui NS et al. (2012) SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study.
    BMJ Open. 2 (6): pii: e001624.
  3. Alhasan, S.F. et al. (2016) Sulfatase-2: a prognostic biomarker and candidate therapeutic target in patients with pancreatic ductal adenocarcinoma.
    Br J Cancer. 115 (7): 797-804.
  4. Phillips, J.J. et al. (2012) Heparan sulfate sulfatase SULF2 regulates PDGFRα signaling and growth in human and mouse malignant glioma.
    J Clin Invest. 122 (3): 911-22.

Immunohistology - Paraffin

Synonyms
SULF-2
RRID
AB_10851205
UniProt
Q8IWU5
Q8CFG0
Entrez Gene
Sulf2
SULF2
GO Terms
GO:0005509 calcium ion binding
GO:0004065 arylsulfatase activity
GO:0005783 endoplasmic reticulum
GO:0005795 Golgi stack
GO:0009986 cell surface
GO:0005615 extracellular space
GO:0030201 heparan sulfate proteoglycan metabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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