Sulfatase 2 antibody | 2B4
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P ResetMouse anti Human Sulfatase 2 (C-Terminal)
- Product Type
- Monoclonal Antibody
- Clone
- 2B4
- Isotype
- IgG1
- Specificity
- Sulfatase 2
- Region
- (C-TERMINAL)
Antibody Quality and 
Unrivaled 
| Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Sulf-2 is linked with several cancers, including breast, hepatocellular, pancreatic and non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50 kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75 kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4 recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide.
- Carrier Free
- Yes
- Immunogen
- Recombinant human Sulf-2.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Sulf-2 transfected HEK29 cells or non-small-cell lung carcinoma.
- Western Blotting
- Mouse anti Human sulfatase 2 antibody, clone 2B4 detects a major band of ~50kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.
References for Sulfatase 2 antibody
-
Lemjabbar-Alaoui, H. et al. (2010) Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis.
Oncogene. 29 (5): 635-46. -
Lui NS et al. (2012) SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study.
BMJ Open. 2 (6): pii: e001624. -
Alhasan, S.F. et al. (2016) Sulfatase-2: a prognostic biomarker and candidate therapeutic target in patients with pancreatic ductal adenocarcinoma.
Br J Cancer. 115 (7): 797-804. -
Phillips, J.J. et al. (2012) Heparan sulfate sulfatase SULF2 regulates PDGFRα signaling and growth in human and mouse malignant glioma.
J Clin Invest. 122 (3): 911-22.
- Synonyms
- SULF-2
- RRID
- AB_10851205
- UniProt
- Q8IWU5
- Q8CFG0
- Entrez Gene
- Sulf2
- SULF2
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004065 arylsulfatase activity
- GO:0005783 endoplasmic reticulum
- GO:0005795 Golgi stack
- GO:0009986 cell surface
- GO:0005615 extracellular space
- GO:0030201 heparan sulfate proteoglycan metabolic process
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